determination of the most suitable live feed for stripped catfish (pangasianodon hypophthalmus) fries during the first 20 days posthatching

CAN THO UNIVERSITY COLLEGE OF AQUACULTURE AND FISHERIES DETERMINATION OF THE MOST SUITABLE LIVE FEED FOR STRIPPED CATFISH (PANGASIANODON HYPOPHTHALMUS) FRIES DURING THE FIRST 20 DAYS POST-HATCHING By HUYNH LE NGOC THU TRAM A thesis submitted in partial fulfillment of the requirements for the degree of Bachelor of Aquaculture Can Tho City, December [DATE] 2012 CAN THO UNIVERSITY COLLEGE OF AQUACULTURE AND FISHERIES DETERMINATION OF THE MOST SUITABLE LIVE FEED FOR STRIPPED CATFISH (PANGASIANODON HYPOPHTHALMUS) FRIES DURING THE FIRST 20 DAYS POST-HATCHING By HUYNH LE NGOC THU TRAM A thesis submitted in partial fulfillment of the requirements for the degree of Bachelor of science in Aquaculture Supervisor Prof Dr.VU NGOC UT Dr TRAN SUONG NGOC Can Tho, January 17th 2013 Acknowledgements I would like to thank the Directory of Can Tho University, the Management Board of the Department of Applied Hydrobiology - College of Aquaculture and Fisheries - Can Tho University for enabling me to study, laboratory assistance, technical support and research topics to complete the program I wish to express my deep appreciation and sincere gratitude to my advisor Prof Dr.Vu Ngoc Ut and Tran Suong Ngoc (Department of Applied Hydrobiology, College of Aquaculture and Fisheries, Can Tho University) for advantage of the guidance, encouragement and valuable advice during the execution of graduation I would like to thank to Ms Hien (rotifer room) was my guide in the rotifer culture to complete the experiment Huynh Le Ngoc Thu Tram i Abstract This research was implemented in the fish hatchery of the College of Aquaculture and Fisheries, Can Tho University from June 2012 to December 2012 Two indoor experiments were conducted for duration of 20 days Tra catfish fries were nursed in 18 L plastic tanks at a density of 10 ind./L The first experiment was designed to determine the most suitable feeding density of Rotifers (Brachionus angularis) The experiment was randomly set up with treatments, replications each Tra catfish fries were fed with different densities (3, 5, and 11 ind./mL) of rotifers only at the first days then Moina was applied toward the end of experiment during 20 days In the second experiment, Tra catfish fries were fed with freshwater rotifers at different times after hatching (24h, 32h, 42h and 48h) corresponding to treatments with replicates each At the end of experiments, all fries were weighed individually to calculate growth and survival rate The result showed that in the first experiment, the most suitable density of rotifers was ind./mL that can improve the survival rate of fish up to 40.19% with the highest growth rate and were statistically different from other treatments (p [...]... replications each The experiments were conducted for duration of 20 days Tra catfish fries were stocked in a 20 L bucket system with a volume of 18L at a density of 10 ind./L 3.3.1 Experiment 1: Determination of the most suitable density of rotifers fed to fish fries In this experiment fish were fed with different densities of rotifers in the first 3 days and then Moina was applied toward the end of experiment... in most trials (S Nandini, 200 4) The experiments were conducted at duration of 30 days with density of 2.5 fry/L Trieu (200 8) evaluated the the effects of different live feed diets on acceptability, growth and survival rate of the fries Whisker sheatfish fries were fed with 4 types of feed including oligochaetes (Tubifex), waterflea (Moina), Artermia nauplii and a combination of Moina and Tubifex The. .. Biotechnol (200 4), feeding rotifers in enriched algae will increase the free Amino Acid (FAA) content of the rotifers They enriched the rotifers for 24 hrs in a microalga medium Enrichment of rotifer with HUFA before using them as live food appears to increase growth and survival of a variety of fish larvae (Lubzens et al., 200 1) According to Ludwig (1994), the optimum feeding rates for most fish larvae... Le Van Cat (200 6), the appropriate temperature for the fish species was from 20 - 30°C Therefore, fish have the ability to grow well in the temperature experiments pH suitable for all fish species from 6.5 to 9.0 (Truong Quoc Phu, 200 6) In this experiment, the pH range from 7.77 to 7.79, suitable for normal physiological activity, growth and development of fish The concentration of NO2- in the fish pond... Quoc Phu, 200 6) In this treatment, NO2- was relatively stable and did not make much difference among treatments because of identical feed Therefore, during the experiment the concentration of NO2- did not affect the growth and development of fish TAN was relatively stable and there was no much difference between the treatments with a range of 0.32 - 0.37 mg/L, entirely suitable for the growth of fish... rate of fish Figure 4.6 Survival rate of fish in all treatments after 20 days in the second experiment 18 CHAPTER V CONCLUSIONS AND RECOMMENDATIONS 5.1 Conclusions: Survival rate of stripped catfish pre-fingerlings can be improved if fed with rotifers (Brachionus angularis) during the first 3 days of rearing and then with water flea (Moina sp.) was applied onward the rearing period The most suitable. .. 0.81 ± 0.29 N-NO2- (mg/L) 0.21 ± 0.04 0 .20 ± 0.04 0.19± 0.04 0.25 ± 0.08 Temperature pH 10 4.2 Mouth gape size: The mouth size of the fish fries at 20 hours after hatching and 3 days in this experiment was 236 ± 23 and 506 ± 19 μm, respectively Within this mouth size rotifers seem to be suitable during the first 3 days After 20 days, the mouth size increased during the sampling intervals and no significant... results showed that the most preferable live feeds for Whisker sheatfish fries were Tubifex or the combination of Moina and Tubifex After 30 days of culture, growth and survival rates of fish in which treatments were significantly higher than that of other treatments (Trieu, 200 8) 6 CHAPTER III MATERIALS AND METHODS 3.1 Location and time: The study was conducted at the College of Aquaculture and Fisheries... Morales-Venture and S S S Sarma (200 4) Functional responses during the early larval stages of the charal fish Chirostoma riojai (Pisces: Atherinidae) fed rotifers and cladocerans 20 Sena S De Silva, (200 7) Status of farming practices of stripped catfish (Pangasianodon Hypophthalmus) in the Mekong Delta, Vietnam Retrieved from 21 Shirota, A (1977) Studies on the mouth size of fish larvae Method and conclusions... successive 17 days with 5 moina/mL Treatment II: 5 rotifers/ml + successive 17 days with 5 moina/mL (control) Treatment III: 8 rotifers/ml + successive 17 days with 5 moina/mL Treatment IV: 11 rotifers/ml+ successive 17 days with 5 moina/mL 7 3.3.2 Experiment 2: Determination of the most suitable start feeding rotifers to fish fries after hatching Tra catfish fries were fed rotifers with the most suitable