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Erba lyte service manual

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Check the liquid positioner: replug the positioner and make sure it is well connected.. -External power supply, power switch, internal power supply works properly - LCD and cables works

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ERBALYTE

Service Manual

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Company Contact Details: ERBA MANNHEIM Mallaustrasse 69-73

68219 Mannheim Germany Tel : (49-6-21) 8799770 Fax : (49-6-21) 8799688

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Preface

In order to operate and service the instrument properly, please read the

operation manual and service manual carefully before operating

When receiving the goods, please check the product model and spare parts

against the packing list If missing or mistake, please contact Erba immediately

Non-authorized personals are not allowed to open or repair the

instrument The instrument must be grounded properly

Never connect the plugs of the rear panel with electric metal materials

Always remember to disconnect the power cable from the instrument

before open it Do not remove any symbols from the instrument

The waste may contain materials harmful to human body It should be

discarded according to the safe laboratory procedures and government regulations

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Contents

Chapter 1 Instrument installation 1

1.1 System introduction 1

1.2 Environment requirements 3

1.3 External Connections 3

1.4 Internal Connections 3

Chapter 2 Power-on check 5

2.1 Self-test and adjustment 5

2.2 Prompts during self-test and calibration 7

Chapter 3 Function description and operation 8

3.1 Flow chart of the operation 8

3.2 System functions 8

3.3 Extended programs: 8

3.4 Routine operation 10

Chapter 4 Routine maintenance 13

4.1 Daily maintenance 13

4.2 Weekly maintenance 13

4.3 Monthly maintenance 13

4.4 Check tubing system 13

4.5 Check the sample probe 17

4.6 Electrode maintenance and replacement 17

4.7 Maintenance of pump, valve and lifting unit 19

Chapter 5 Hardware 21

5.1 Connector and Cable list 21

5.2 PCB errors 21

5.3 Description of RS-232 interface 22

Chapter 6 Trouble-shooting 24

6.1 Error prompts 24

6.2 Possible causes of errors 25

6.3 Trouble-shooting of positioner and electrode 26

6.4 Slope unstable 26

6.5 Slope abnormal 27

6.6 AB slope and result abnormal 27

6.7 Auto sampler trouble shooting 27

6.8 Aspiration abnormal 28

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Chapter 1 Instrument installation

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1.2 Environment requirements

1 The instrument should be installed on a stable and solid platform that is free of

mechanical vibration and away from vibration source

2 The environment should be as free as possible from dust, corrosive gas, loud noises and electrical interference

3 Avoid placing the instrument in direct sunlight or in front of a source of heat or vent

4 Ambient temperature: 10~35 , relative humidity:20%-85%

5 The power supply should be AC220/110V±10%, 50/60 Hz

6 Power supply and grounding should be connected correctly

7 No strong electric field and magnetic field interference

3 If the power supply to the instrument is not stable, or there are high-power equipments

on the same line, then a power stabilizer (output ≥100W) is strongly suggested

4 Avoid operating the instrument under high humidity environment for long time, otherwise the instrument performance may be affected Use desiccant or air conditioner in case of necessary Do not place the instrument near a water pool

1.4 Internal Connections

Press the front door to open it

1 Pump tube installation: put the aspirating pump tube and the lactic pump tube

on the pump head, fix the tube connectors to the bracket, adjust the tube to the

middle of the pump head

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2 Check the electrode assembly: take out the electrode assembly from the accessory kit Check if the setting screws are tightened, inlet and outlet port are clean, the filling solution of each electrode is sufficient If there are bubbles on the electrode probe, tap the electrode slightly to let the bubbles disappear

3 Installation of the electrode assembly: insert the whole assembly to the mounting base, connect the lead wire of each electrode (K、Na、Ca、PH、CL、Ref) to the plug of amplifier board correctly Connect the tube to the inlet and outlet port of the assembly

4 Check the liquid positioner: replug the positioner and make sure it is well connected

5 Check the tubing: make sure all the tubings are connected tightly and there is no leaking

6 Connect to the reagent pack: connect the Std A tube to port A of the reagent pack Connect the Std B tube to port B Connect Std R tube to port R Connect the waste tube to port W

7 Installation of printing paper: open the printer cover, insert the printing paper into the guide slot (the thermal side face down), feed the paper out from the slot on the printer cover

Note:

a Make sure all tubings are connected firmly and there is no leakage

a Make sure the positioner is connected firmly

a Make sure all the lead wires of the electrodes are connected firmly

a Make sure there is no bubble on the electrode probes

a The printing paper is using out when you see two red lines on it Change

the printing paper in time

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Chapter 2 Power-on check

2.1 Self-test and adjustment

When switched on the power to the instrument, a system self-test will be performed automatically The operator may be required to do some adjustments to ensure the instrument operates satisfactorily under given conditions

Switch on the power,

the LCD light on -External power supply, power switch, internal power

supply works properly

- LCD and cables works properly

-External power supply, power switch connections or fuse is not good

- Internal power supply doesn’t work properly

Detect the positioner,

printer and auto

sampler(if connected)

- The positioner works properly

- The printer works properly

- Auto sampler works properly

- The positioner loose

- The printer cables or internal power supply is not good

- The system will not give alarm or stop even

if errors found on the positioner or printer The operator should turnoff the instrument manually and solve the problem

Feed paper and

print-out the system version

- The power supply and paper feeding of the printer is good

- Data transmission of the printer is good

- Internal power supply

is not good

- The printing paper

is not installed correctly

- The printer cables are loose or not good

Sample probe moves

up until it touches

the position limit

switch, then moves

down to the wash

position during the movement

- The lifting mechanism is not good

- Adjust or replace the sample probe if

necessary

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Calibrating … -The liquid distribution valve

and optical coupler, lifting mechanism, switches and software works properly

-The system gives alarm and stops if errors

- Pump motor works properly

- The tubing system is good if Std B and Std A can be aspirated into the distribution valve correctly -Details of voltage display and print please refer to the operation manual

- The operator can turn off the function of voltage display or print by extended program

-Pump motor is malfunction

- The liquid cannot reach correct position, showing the tubing system is leaking

- The inlet tubes for Std A and Std.B are connected to the wrong ports

- Std A or Std B is not aspirated

Screen display after

detection and data processing work normally

- If slope is not stable, then it shows “shift”

- If slope is abnormal, then it shows “X”

-Refer to trouble-shooting for the reason of unstable or abnormal slope

The system enters

into main menu after

self-test

MainMenu Sample Cal W List STD Service Results

If there is no

operation for more

than 2 minutes, the

system will enter

into standby mode

- the internal clock works normally - The malfunction if the internal clock is

system can not enter into standby mode

Note:

Try the other side of the printing paper or change the whole paper roll if there is no

printing on the paper Adjust the position of the paper if it is misalignment

If the sample probe is found distortion, misaligned or bended, the operator should

adjust or change the probe and make sure it can move into the wash block correctly

If the aspiration is abnormal, the operator should check the tubing

connections, reagent connections and electrode connections and make sure there

is no blockage or leakage

Activate the electrode for 10-30 minutes before using if the electrode has

been stored or not used for a long time Run calibration when electrode is stable

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-2.2 Prompts during self-test and calibration

2.2.1 Display Prompts

1 The optical coupler of liquid distribution valve failed

2 Elevator switch failed

2.2.2 Print Prompts

Error 0# Liquid positioning failed

Error 1# Liquid detecting failed

Warn 2# Bubbles detected

Error 3# Too much or less sample aspirated

Error 4# Slope abnormal

Error 5# Slope unstable

Error 6# AB Slope abnormal

Error 7# AB Slope unstable

For the solutions please refer to “Trouble-shooting”

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Chapter3 Functional description and operation

3.1 Flow chart of operation

Please refer to the operator’s manual

extended program menu, the screen displays:

(Attention: the extended programs are not open for the end-users to avoid wrong setting)

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TCa is a calculated parameter

Press Pr.TCa , the button turns white The value of iCa and TCa will be printed out when the sample measurement is completed

Press Pr.TCa again, the button turns white Only the value of iCa will be printed out when the sample measurement is completed This is the default setting

3.3.4 Adjust pH

This function has been fixed at factory, no need to adjust it again

3.3.5 Standby mode setting

The instrument will enter into Standby mode if there is no operation in 2 minutes In standby mode, the operator should press Wake Up to activate the instrument to the working mode If the instrument stays in standby mode for 4 hours, it will maintain the electrodes automatically When wake up the instrument, it will carry out a calibration before returning to the working mode

The operator can change the waiting time for entering into standby mode The suggested waiting time is 2-60 minutes

To change the waiting time, press Standby ,input the value, then press Yes to confirm

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3.3.7 Show voltage

Press Showmv, the button turns white The voltages of the liquid positioner and all

electrodes will be displayed on the screen during calibration or sample measurement

Press Show mv again, the button turns white The voltages of the liquid positioner and all electrodes will not be displayed on the screen during calibration or sample measurement

3.3.8 Setup the reference range

Touch Range, the screen displays:

PressSet, input all the reference values in turn,thenpress Exit to quit

3.3.9 Record of de-protein operation

Press Pr.Clea to print out the last date that the de-protein program been performed

3.4 Routine operation

3.4.1 Safety precautions

1 The electric voltage inside the instrument may be harmful to human body

Do not open the instrument without disconnecting it from the power supply

2 Because the samples may have pathogenesis bacterium or viruses, all replaced tubes, electrodes and waste containers should be discarded according to the safe laboratory procedures and government regulations

3 The reagents are irritating to eyes, skin and diaphragm Wear proper personal protective equipment (e.g.gloves, lab coat, etc.) and follow safe laboratory procedures when

handling them in the laboratory

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4 The reagents are harmful to human body If the reagents accidentally spill on your skin, wash them off with plenty of water and if necessary, go to see a doctor

5 If the reagents accidentally spill into your eyes, wash them off with plenty of water and

immediately go to see a doctor

3.4.2 Sample Collection and handling

Sample collection and handling must be carried out by the professionals Always avoid the hematolysis In addition, the following points should be noted:

1 The serum or plasma can be stored in the refrigerator, but they must be warmed up

to room temperature before test

2 When preparing the blood serum samples, do not add any materials like the surface active agent that may interfere in the measurement or even damage the sensor

3 Hemolysis can affect K result significantly as well as other parameters Special remark should be made for hemolysis sample, and the results are for reference to the lab only

4 If the serum has not been separated completely, the fibrins in the sample may affect the aspiration, and the proteins may deposit inside the tubing thus causing contamination

or blockage In such a case, the results will be affected, so the operator should clean the tubing on time

5 The sample should be tested within 2 hours, otherwise the result may be not accurate

5 When the sample reaches the checking point, make sure there are no bubbles

inside, otherwise the samples should be measured again

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6 Never aspirate the sample directly from the blood separating tube, because

it is very easy to block the tubing system Always move the serum to a small and clean tube or cup with a pipette before testing on the instrument

7 If the ambient temperature fluctuates for more than 10 , the instrument should be calibrated again

8 The pH value of the standard solutions and the samples should be within 6-9, otherwise it will interfere in the measurement of sodium ions

9 Discard the reagent if mild dew or deposition found

10 Perform the routine maintenance according to the manual

11 Every electrode has a serial number Keep it on the electrode The warranty of the electrodes will become invalid if the number is not mentioned

12 When removing the sample tube after aspiration, be careful not to hit the sample probe, otherwise the probe will bend or get misaligned and may not move into the wash block properly

13 Do not open the front door during calibration or sample measurement, otherwise the result will be affected

14 Check and make sure the grounding is good if static interference is found

3.4.4 Daily operation

Please refer to the operator’s manual

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