Erba lyte service manual

Service Manual ERBALYTE Company Contact Details: ERBA MANNHEIM Mallaustrasse 69-73 68219 Mannheim Germany Tel : (49-6-21) 8799770 Fax : (49-6-21) 8799688 Preface In order to operate and service the instrument properly, please read the operation manual and service manual carefully before operating. When receiving the goods, please check the product model and spare parts against the packing list. If missing or mistake, please contact Erba immediately. Non-authorized personals are not allowed to open or repair the instrument. The instrument must be grounded properly. Never connect the plugs of the rear panel with electric metal materials. Always remember to disconnect the power cable from the instrument before open it. Do not remove any symbols from the instrument. The waste may contain materials harmful to human body. It should be discarded according to the safe laboratory procedures and government regulations. -i- Contents Chapter 1. Instrument installation ....................................................................... 1 1.1 System introduction ........................................................................................ 1 1.2 Environment requirements ...............................................................................3 1.3 External Connections ...................................................................................... 3 1.4 Internal Connections ....................................................................................... 3 Chapter 2. Power-on check ................................................................................... 5 2.1 Self-test and adjustment .................................................................................. 5 2.2 Prompts during self-test and calibration........................................................... 7 Chapter 3. Function description and operation ..................................................... 8 3.1 Flow chart of the operation ............................................................................... 8 3.2 System functions .............................................................................................. 8 3.3 Extended programs: ......................................................................................... 8 3.4 Routine operation ............................................................................................ 10 Chapter 4. Routine maintenance .........................................................................13 4.1 Daily maintenance........................................................................................... 13 4.2 Weekly maintenance ........................................................................................ 13 4.3 Monthly maintenance...................................................................................... 13 4.4 Check tubing system ....................................................................................... 13 4.5 Check the sample probe .................................................................................. 17 4.6 Electrode maintenance and replacement ......................................................... 17 4.7 Maintenance of pump, valve and lifting unit ................................................... 19 Chapter 5. Hardware ........................................................................................... 21 5.1 Connector and Cable list ................................................................................ 21 5.2 PCB errors...................................................................................................... 21 5.3 Description of RS-232 interface....................................................................... 22 Chapter 6 Trouble-shooting ................................................................................ 24 6.1 Error prompts ................................................................................................ 24 6.2 Possible causes of errors ................................................................................ 25 6.3 Trouble-shooting of positioner and electrode................................................... 26 6.4. Slope unstable .............................................................................................. 26 6.5 Slope abnormal .............................................................................................. 27 6.6 AB slope and result abnormal ........................................................................ 27 6.7 Auto sampler trouble shooting. ...................................................................... 27 6.8 Aspiration abnormal ....................................................................................... 28 - ii - Chapter 1. Instrument installation 1.1. Rear panel 1. Power switch 2. Power 3. RS-232 serial port 4. Grounding pole 5. Machine label socket (including fuse holder) -2- 1.2 Environment requirements 1. The instrument should be installed on a stable and solid platform that is free of mechanical vibration and away from vibration source. 2. The environment should be as free as possible from dust, corrosive gas, loud noises and electrical interference. 3. Avoid placing the instrument in direct sunlight or in front of a source of heat or vent. 4. Ambient temperature: 10~35 , relative humidity:20%-85%. 5. The power supply should be AC220/110V±10%, 50/60 Hz 6. Power supply and grounding should be connected correctly. 7. No strong electric field and magnetic field interference. 1.3 External Connections 1. Power cable: should be connected to the power socket firmly and reliably without loose or poor connection. 2. Grounding: the grounding pole should be connected to ground properly. The grounding wire should be as short as possible and the grounding resistance must be less than 10Ω 3. If the power supply to the instrument is not stable, or there are high-power equipments on the same line, then a power stabilizer (output ≥100W) is strongly suggested. 4. Avoid operating the instrument under high humidity environment for long time, otherwise the instrument performance may be affected. Use desiccant or air conditioner in case of necessary. Do not place the instrument near a water pool. 1.4 Internal Connections Press the front door to open it. 1. Pump tube installation: put the aspirating pump tube and the lactic pump tube on the pump head, fix the tube connectors to the bracket, adjust the tube to the middle of the pump head. -3- 2. Check the electrode assembly: take out the electrode assembly from the accessory kit. Check if the setting screws are tightened, inlet and outlet port are clean, the filling solution of each electrode is sufficient. If there are bubbles on the electrode probe, tap the electrode slightly to let the bubbles disappear. 3. Installation of the electrode assembly: insert the whole assembly to the mounting base, connect the lead wire of each electrode (K、Na、Ca、PH、CL、Ref) to the plug of amplifier board correctly. Connect the tube to the inlet and outlet port of the assembly. 4. Check the liquid positioner: replug the positioner and make sure it is well connected. 5. Check the tubing: make sure all the tubings are connected tightly and there is no leaking. 6. Connect to the reagent pack: connect the Std A tube to port A of the reagent pack. Connect the Std B tube to port B. Connect Std R tube to port R Connect the waste tube to port W. 7. Installation of printing paper: open the printer cover, insert the printing paper into the guide slot (the thermal side face down), feed the paper out from the slot on the printer cover. Note: a Make sure all tubings are connected firmly and there is no leakage. a Make sure the positioner is connected firmly. a Make sure all the lead wires of the electrodes are connected firmly. a Make sure there is no bubble on the electrode probes. a The printing paper is using out when you see two red lines on it. Change the printing paper in time. -4- Chapter 2. Power-on check 2.1 Self-test and adjustment When switched on the power to the instrument, a system self-test automatically. will be performed The operator may be required to do some adjustments to ensure the instrument operates satisfactorily under given conditions. Operation Function properly Switch on the power, the LCD light on. Possible problems -External power supply, power -External switch, internal supply works properly - LCD and cables works properly power supply, power power switch connections or fuse is not good. - Internal power supply doesn’t work properly. Detect the positioner, printer and auto sampler(if connected) - The positioner works properly - The positioner loose. - The printer works properly - The printer cables or internal power supply is - Auto sampler works properly not good - The system will not give alarm or stop even if errors found on the positioner or printer. The operator should turnoff the instrument manually and solve the problem. Feed paper and print- - The power supply and paper out the system version - Internal power supply feeding of the printer is good - Data transmission of the printer is good is not good - The is printing paper not installed correctly. - The printer cables are loose or not good. Sample probe moves - The lifting motor, limit switch up until it touches the position switch, then down to and cables work properly. limit Check moves probe if the wash position during the movement. -5- not good sample - Adjust or replace the is bended or out of block ¶ the - The lifting mechanism is sample probe if necessary Calibrating … -The liquid distribution valve -The system gives alarm and optical coupler, lifting and mechanism, switches and found software works properly. stops if errors on liquid distribution valve, lifting mechanism or switches. The instrument - Pump motor works properly -Pump motor is malfunction aspirates Std B and - The tubing system is good if - The Std A in turn for test and display the voltages the of voltage display - The inlet tubes for Std A and operation manual connected function turn of off system self-test If there is are to the wrong ports valve, aspirated A, - If slope is not stable, then it shows “shift” signal - If slope is abnormal, detection and data then it shows “X” processing work normally enters into main menu after Std B, tubing connections, pump, Std.B voltage - Std A or Std B is not program. after - All electrodes, Std tubing system is leaking display or print by extended The the and print please refer to the the OK OK OK OK OK position, showing into - The operator can 54.5 52.3 51.6 25.5 55.6 correct reach aspirated -Details Slope K： Na： Cl： Ca： pH： cannot Std B and Std A can be distribution valve correctly Screen display calibration: liquid -Refer to trouble-shooting for the reason unstable or abnormal of slope MainMenu Sample Cal. W. List STD Service Results no - the internal clock works more normally - The internal clock is malfunction if the than 2 minutes, the system can not enter system into operation for will enter into standby mode standby mode Note: Try the other side of the printing paper or change the whole paper roll if there is no printing on the paper. Adjust the position of the paper if it is misalignment. If the sample probe is found distortion, misaligned or bended, the operator should adjust or change the probe and make sure it can move into the wash block correctly. If the aspiration connections, is abnormal, the operator should check the tubing reagent connections and electrode connections and make sure there is no blockage or leakage. Activate the electrode for 10-30 minutes before using if the electrode has been stored or not used for a long time. Run calibration when electrode is stable. -6- 2.2 Prompts during self-test and calibration 2.2.1 Display Prompts Code 2.2.2 Error 1 The optical coupler of liquid distribution valve failed 2 Elevator switch failed Print Prompts Code Error Error 0# Liquid positioning failed Error 1# Liquid detecting failed Warn 2# Bubbles detected Error 3# Too much or less sample aspirated Error 4# Slope abnormal Error 5# Slope unstable Error 6# AB Slope abnormal Error 7# AB Slope unstable For the solutions please refer to “Trouble-shooting” -7- Chapter3. Functional description and operation 3.1 Flow chart of operation Please refer to the operator’s manual. 3.2 System functions Please refer to the operator’s manual. 3.3 Extended programs: Press Service in the main menu, press Time and enter “983”, press Exit, then the system will enter into extended program menu, the screen displays: (Attention: the extended programs are not open for the end-users to avoid wrong setting) 3.3.1 Print voltage Press Pr.Vol , the button turns white. The voltages of the liquid positioner and all electrodes will be printed out during calibration or sample measurement. Press Pr.Vol again, white color disappear from the button. The voltages will not be printed out During calibration or sample measurement. This is the default setting. -8 3.3.2 Print PH value Press Pr.PH , the button turns white. The pH value will be printed out when the sample measurement is completed. Press Pr.PH again, the button turns dark. The pH value will not be printed out when the sample measurement is completed. This is the default setting. 3.3.3 Print TCa TCa is a calculated parameter. Press Pr.TCa , the button turns white. The value of iCa and TCa will be printed out when the sample measurement is completed. Press Pr.TCa again, the button turns white. Only the value of iCa will be printed out when the sample measurement is completed. This is the default setting. 3.3.4 Adjust pH This function has been fixed at factory, no need to adjust it again. 3.3.5 Standby mode setting The instrument will enter into Standby mode if there is no operation in 2 minutes. In standby mode, the operator should press Wake Up to activate the instrument to the working mode. If the instrument stays in standby mode for 4 hours, it will maintain the electrodes automatically. When wake up the instrument, it will carry out a calibration before returning to the working mode. The operator can change the waiting time for entering into standby mode. The suggested waiting time is 2-60 minutes. To change the waiting time, press Standby ,input the value, then press Yes to confirm. 3.3.6 Test Valve The liquid distribution valve may be blocked or leaking after using for a long time. The measuring results could be affected. Therefore it is necessary to check the valve periodically. Touch Valve, the system will aspirate Std A and Std B automatically. Observe the aspiration volume, flow speed, the switch of Std.A/Std.Bin the valve and the bubbles to determine if the valve is working properly or not. -9- 3.3.7 Show voltage Press Showmv, the button turns white. The voltages of the liquid positioner and all electrodes will be displayed on the screen during calibration or sample measurement. Press Show mv again, the button turns white. The voltages of the liquid positioner and all electrodes will not be displayed on the screen during calibration or sample measurement. 3.3.8 Setup the reference range Touch Range, the screen displays: K 1 xxx 2 xxx Na 3 xxx 4 xxx Cl 5 xxx 6 xxx ica Tca 7 xxx 8 xxx 9 xxx 10 xxx PH Set Exit PressSet, input all the reference values in turn,thenpress Exit to quit. 3.3.9 Record of de-protein operation Press Pr.Clea to print out the last date that the de-protein program been performed. 3.4 Routine operation 3.4.1 Safety precautions 1. The electric voltage inside the instrument may be harmful to human body. Do not open the instrument without disconnecting it from the power supply. 2. 3. Because the samples may have pathogenesis bacterium or viruses, all replaced tubes, electrodes and waste the procedures safe laboratory containers should be discarded according to and government regulations. The reagents are irritating to eyes, skin and diaphragm. Wear proper personal protective equipment (e.g.gloves, lab coat, etc.) and follow safe laboratory procedures when handling them in the laboratory. - 10 - 4. The reagents are harmful to human body. If the reagents accidentally spill on your skin, wash them off with plenty of water and if necessary, go to see a doctor. 5. If the reagents accidentally spill into your eyes, wash them off with plenty of water and immediately go to see a doctor 3.4.2 Sample Collection and handling Sample collection Always avoid 1. and handling must be carried out by the professionals. the hematolysis. In addition, the following points should be noted: The serum or plasma can be stored in the refrigerator, but they must be warmed up to room temperature before test. 2. When preparing the blood serum samples, do not add any materials like the surface active agent that may interfere in the measurement or even damage the sensor. 3. Hemolysis can affect K result significantly as well as other parameters. Special remark should be made for hemolysis sample, and the results are for reference to the lab only. 4. If the serum has not been separated completely, the fibrins in the sample may affect the aspiration, and the proteins may deposit inside the tubing thus causing contamination or blockage. In such a case, the results will be affected, so the operator should clean the tubing on time. 5. The sample should be tested within 2 hours, otherwise the result may be not accurate. 3.4.3 Operation precautions 1. The analyzer is designed to work continuously for 24 hours a day. No need to shut down the machine everyday. 2. Do not use the standard solutions of flame photometer. They include strong acid and other supplements that may damage the electrodes. 3. Not all commercial controls are suitable for ISE measurement. Some of them contain too much chemical additives that may interfere in the measurement. 4. The bubbles should not be aspirated during sample aspiration, otherwise the results will be unreliable. 5. When the sample reaches the checking point, make sure there are no bubbles inside, otherwise the samples should be measured again. - 11 - 6. Never aspirate the sample directly from the blood separating tube, because it is very easy to block the tubing system. Always move the serum to a small and clean tube or cup with a pipette before testing on the instrument. 7. If the ambient temperature fluctuates for more than 10 , the instrument should be calibrated again 8. The pH value of the standard solutions and the samples should be within 6-9, otherwise it will interfere in the measurement of sodium ions. 9. Discard the reagent if mild dew or deposition found. 10. Perform the routine maintenance according to the manual. 11. Every electrode has a serial number. Keep it on the electrode. The warranty of the electrodes will become invalid if the number is not mentioned. 12. When removing the sample tube after aspiration, be careful not to hit the sample probe, otherwise the probe will bend or get misaligned and may not move into the wash block properly. 13. Do not open the front door during calibration or sample measurement, otherwise the result will be affected. 14. Check and make sure the grounding is good if static interference is found. 3.4.4 Daily operation Please refer to the operator’s manual. - 12 - Chapter 4. Routine maintenance 4.1 Daily maintenance Keep the instrument clean, especially the sampling unit. 4.2 Weekly maintenance 1. Check the electrodes and make sure the filling solutions are sufficient. Add filling solution if the volume is less than 2/3. 2. Check if there is salty crystal on the internal electrode, if so, clean it with wet tampon. 3. Check and clean the rubber in the wash block. Clean the sample probe. 4. Run deprotein program in service menu. (If less than 5 samples per day, then just run deprotein program every 2 weeks) Check and clean the tubing system. 5. Run “Na adjust” program if the slope of Na electrode is less than 50. 4.3 Monthly maintenance 1. Check the tubing system and make sure there is no blockage or leakage. 2. Check the internal electrode and make sure it doesn’t blanch or its coating doesn’t flake off. Change the internal electrode if necessary. Check the filling solution, change it if it become contaminated or dirty. 3. Observe the voltage of the electrode. If the voltage of K,、Na,、Cl are all less than 20 mV, then change the reference filling solution first. If it doesn’t improve, change the reference membrane. 4. Run calibration if the slopes are low. 4.4 Check tubing system Check the tubing system if the aspiration volume or flow speed is abnormal, or bubbles found. Observe the flow speed, liquid position and bubble status during sample test or calibration. 1. Blockage will cause insufficient aspiration volume for sample, Std A and Std B, or no aspiration at all if the blockage is very bad. - 13 - 2. Leakage will cause bubbles or no aspiration. 3. A segment of air will appear in the tube when the liquid distribution valve switches between Std A and Std B. When aspirating Std A, if small bubbles found at the outlet of distribution valve, then the flow path inside the distribution valve for Std A is leaking. If bubbles always stay at the inlet of Std A and can not be excluded, then the flow path for Std A is blocked slightly. Check flow path for Std B by the same way. 4. The blockage in the distribution valve is usually caused by contamination in Std A or Std B. It is important to keep the reagents under proper temperature. 5. The bubbles will appear near the connector if it is leaking. Tighten the tube or connect it again. Cut a small piece of tube if necessary. 6. If leakage found between the electrodes, then the O ring between the electrodes may not be present or maybe out of position, or the setting screws might not be not tightened. 7. If the pump tube is distorted, leaking or aged, it will cause no aspiration, insufficient aspiration volume or incorrect liquid position. Change the pump tube if necessary. 8. Wash the tubing system regularly to prevent the blockage caused by protein deposition. Clean the sample probe externally. Clean the wash block. - 14 - 4.5 Check the sample probe 1. Adjust the sample probe if it is misaligned or bend. Make sure the sample probe can move into the wash block smoothly and correctly. 2. If the sample probe is blocked, move it up to top position, take the probe out, and use a thin needle to remove the blockage from the probe. 3. If the wash block is blocked, lift up the sample probe, remove the cover of the wash block, take out the rubber and clean it. 4.6 Electrode maintenance and replacement 4.6.1 Structure of the electrode (For Erba lyte plus there will not be Ca & PH electrode) 4.6.2 Replacement of internal electrode and filling solution Follow the steps below to replace the internal electrode or filling solution: 1. Pull out all electrode wires from the plugs. Remove the tubes from the inlet and outlet of the electrode assembly. 2. Loose the fixing nuts of the electrode assembly. 3. Disassemble the whole electrode assembly. 4. Discard the electrode to be replaced. 5. Take a new electrode and add the filling solution. 6. Assemble the electrodes in correct order. Make sure that the rubber gaskets are in right position. - 17 - 7. Tighten the fixing nuts of the electrode assembly. 8. Connect the tubing to inlet and outlet. Insert the electrode wires back to the plugs. 9. Run calibration again. If the internal electrode blanches or its coating flakes off, then remove the old filling solution, add fresh filling solution and install a new internal electrode. Change the filling solution if it is insufficient or the slope of the electrode is too low. Take out the internal electrode (do not touch any metal materials), remove the old filling solution, add fresh solution to 85% of total volume, thenput the internal electrode back and tighten it. K filling solution is for K electrode only, Ca filling solution is for Ca electrode only, and reference filling solution is for reference electrode only. Na/Cl filling solution is for Na, Cl and pH electrodes. 4.6.3 Replace the reference membrane Follow the steps below to replace the reference membrane: 1. Pull out all electrode wires from the plugs. Remove the tubes from the inlet and outlet of the electrode assembly. 2. Loose the fixing nuts of the electrode assembly. 3. Take out the reference electrode. 4. Loose the screw cap, take out the internal electrode, remove the filling solution, O- 5. Soak 6. Refill the reference filling solution and tighten the screw cap 7. Clean and dry the electrode. ring and old reference membrane (see the following figure). evenly 8. the on new the reference membrane in distilled water, then place it internal interface and put on the O-ring. Assemble the electrodes in correct order. Make sure that the rubber gaskets are in right position. 9. Connect the tubing to inlet and outlet. Insert the electrode wires back to the plugs. 10. Run calibration again. - 18 - 4.7 Maintenance of pump, valve and lifting unit 4.7.1 Maintenance of aspirating pump After long time of using, the pump tube will get aged and may cause inaccurate aspiration volume and result in bad results. So, it is necessary to change the pump tube. If there is big noise when the pump head rotating, disconnect the pump tube, pull out the pump head, loose three setting screws, take out the plate, add a little lubrication oil to the ball bearing, then assemble the pump head back. If the pump head is damaged, then change it with a new one. 4.7.2 Maintenance of distribution valve The function of the distribution valve is to switch the flow path to Std A or Std B according to the control program. The valve status is detected by an optical coupler. The performance of the optical coupler can be affected by dust or foreign objects, and may cause wrong switching between Std A and B. In such case,“1#”、”2#” or “3#” error message will be shown on the screen and will be printed out. Clean the optical coupler periodically. Disassemble the valve: Loosen the four setting screws on the valve plate, pull the whole valve out a little, loosen four nuts, take out the valve plate and four copper standings, loose the setting screws for the optical coupler. Take out the optical coupler. Turn the valve head with a tweezers and take it out. Loose two screws on the valve cap and take the cap out. Clean five O rings on the valve cap. Clean the valve body and rotor. Assemble the valve: Install the rotor and cap to the valve body, tighten two screws. Pay attention to the five O rings on the cap. Install the optical coupler to center position. Put on the valve head, tighten the screws on the valve motor axis. Put the copper standings back, tighten the valve body to the plate. Tighten the valve plate to instrument. Note: Pay attention to the position of the O-rings, copper standings, optical coupler and connectors of Std.A and Std.B - 19 - Maintain the valve once every year. Keep the optical coupler clean and check it periodically. 4.7.3 Lifting unit The maintenance of the lifting unit is by removing the dust, foreign objects and oil dirt from it. Remove the cover of the sample probe, remove the sample probe, clean the axis and add a little grease. Let the sample probe move up and down for several times, make sure it moves smoothly and low noise. Note: The electrode should be sealed and stored under dry environment. Do not add filling solution to it. The tubing and O-rings should be sealed and stored under dry environment. The internal electrode should be sealed and stored under dry environment. Do not store with metal materials. - 20 - Chapter 5. Hardware 5.1 Connector and Cable list Connector Cable Connector Cable J101 Optical coupler cable J203 Positioner cable J102 Elevator switch cable J204 Signal cable J103 Auto sampler home position optical coupler Auto sampler counter optical coupler cable J205 RS232 cable J206 Pressure sensor cable J104 J107 Transformer output cable J207（PRT） Printer data cable J108 Printer power cable J207（Key） Touch screen cable J109 Air outlet valve connection cable J208 LCD cable NBQ LCD inverter power cable J110 Liquid outlet valve connection cable J111 Mixing motor supply cable J112 Auto sampler motor cable J114 Lifting motor cable J115 Valve motor cable J116 Aspirating Pump motor bl Lactic acid pump motor cable J117 power 5.2 PCB errors 1. Because of vibration or dust accumalation, the connectors or IC chips may have loose connections. Disconnect the power cable, open the rear cover, and check the cables and IC chips. - 21 - 2. When after switch on the power, the LCD doesn’t light on, the lifting unit doesn’t work, the pump motor doesn’t work: check 9-pin DC power cable and transformer output cable J107. 3. When switch on the power, the LCD doesn’t light on, but the lifting unit work normally: Check U102 (LM7812)、9-pin DC power cable, NBQ connector and 12V voltage. The LCD inverter may be damaged. 4. The lifting unit can not move, the valve doesn’t work: Check U103 (LM7812)、 9-pin DC power cable、U106、U107. 5. The pump motor doesn’t work: Check U110、LM7812、9-pin DC power cable、U108、 U109 6. The signals of calibration or measurement are confused or all zero: check cable J204. 7. If the program works incorrect, clean the dust accumulated around U301 IC chip and check. It is recommended to clean PCB with pure alcohol and blow to dry. 8.）Auto sampler doesn’t move, check U104, 74HC373,U105, ULN2803A. 9.）No AB test result, check J206 connector and U209, LM324. 10.）No print. Check J108, J207(PRT) connectors and the connectors between printer head and print control board. Replace the printer head if it is broken. Note: Only authorized personnels are allowed to repair the PCB. 5.3 Description of RS-232 interface The analyzer can send or receive command/data via RS-232 interface. 5.3.1 Features Electric feature： comply with EIA RS232C Transmission mode： asynchronism Stopbit 1digit Databit 8digit Paritybit none Transmission speed： 19200 baud - 22 - 5.3.2 Pin description Pin no. Signal Functio Note 1 NC none 2 RXD Receive data Input 3 TXD Transmit data Output 4 NC none 5 GND signal ground 6 NC none 7 NC none 8 NC none 9 NC none 5.3.3 Data format The instrument communicates with external PC through RS-232 serial port. After each test finish, the data will be automatically sent out during the time of printing. The content of the transmitting data is almost same as the printing data. The operator can also send out the data manually by “Send” program in “Service” menu. Example: 001 111111 063 5.23 151.2 111.4 1.44 7.62 12.0 004 112224 063 5.25 151.6 110.8 1.40 7.59 42.0 Data format: Sample number + 2 blank + ID + 2 blank + symbol byte + blank + k result + blank + Na result + blank + Cl result + blank + Ca result + blank + PH result + blank + AB result + newline Note: Symbol byte: this byte has 8 digits h g f e d c b a a =1: K electrode calibration failed, a =0: K electrode calibration OK b =1: Na electrode calibration failed, b =0: Na electrode calibration OK c =1: Cl electrode calibration failed, c =0: Cl electrode calibration OK - 23 - d =1: Ca electrode calibration failed, d =0: Ca electrode calibration OK e =1: pH electrode calibration failed, e =0: pH electrode calibration OK f =1: AB calibration failed, f =0: AB calibration OK h =1: warning information of this result (e.g. aspiration abnormal, bubbles detected, etc.) Chapter 6 Trouble-shooting 6.1 Error prompts Display prompts Code 1# Error Solution Optical coupler error Check J101, clean optical coupler, adjust the position of optical coupler 2# Elevator switch error Check J102, J114, elevator switch, U103(LM7812)、 U107(ULN2803)、U106(74HC373) ,9-pin DC power cable Printing prompts Code Error 0# Error Liquid Solution positioning Connect the positioner again. failed Error 1# Liquid detecting failed Check the optical coupler and tubing connection of the distribution valve Error 2# Bubbles detected Check the positioner, optical coupler, distribution valve, check if there is any leakage in the tubing sstem Error 3# Too much or less Check the distribution valve and positioner sample aspirated Error 4# Slope abnormal Refer to “6.5 Slope abnormal” Error 5# Slope unstable Refer to “6.4 Slope unstable” - 24 - 6.2 Possible causes of errors ( 1 ) Power supply not good be good: May cause the instrument to work abnormally; the slopes become abnormal during calibration; the testing result not stable. (2) Grounding not good May cause：result not stable during testing or calibration, especially for Na. (3) Interfere by electromagnetism May cause：the instrument can not work normally; result not stable during testing or calibration (4) Interference due to static and dry condition May cause: instrument works unstable, the deviation of Na, Cl is too big (5) Interfere due to moisture May cause: instrument works unstable, the deviation of Na is too big (6) Interference due to temperature May cause: instrument works unstable. (7) Positioner not good May cause inaccurate liquid positioing, prompt “bubbles” frequently, slope abnormal or unstable, testing result unstable. (8) Flow path in the electrode assembly contaminated May cause：slope abnormal or too low (9) Aspirating tube contaminated May cause：slope abnormal, especially for K electrode (10) Std A or Std B contaminated May cause：slope not stable or too low (11) Reference electrode not good May cause unstable slope hence unstable results. (12) Flow channel not good Check segment by segment. - 25 - 6.3 Trouble-shooting of positioner and electrode Positioner: First, make sure the connection of the positioner is good. Then check the positioner’s voltage with “Voltage” program in “Service” menu. Rotate the pump manually and let the liquid pass through the positioner. The positioner’s voltage should become small when there is liquid or big when there is no liquid. The difference should be more than 500. Electrode: Check the voltage of the electrode by “Voltage” program in “Service” menu. If the voltage is low, then the electrode should be replaced as soon as possible. If aspiration alarm appeared or no aspiration at all, replace a new reagent pack (Std A, Std B) Restart the instrument and run self-test. 6.4. Slope unstable Cause Solution Unreliable grounding Check the connection of the grounding wire No standard A or standard B Aspirated Check and replace Standard A or B; Check the tubing connection Incorrect positioning Adjust positioning again The reference filling solution or reference membrane not working Replace when necessary The internal electrode turns gray Replace when necessary Poor connection of the electrode wire Check and connect again Electrode membrane leaks Replace when necessary Power supply voltage fluctuates Use UPS or power stabilizer Humidity too high Lower the humidity or move the instrument to a dry place Bubbles inside the tubing Check the tubing system Liquid leakage inside the valve Replace gasket or valve when necessary The electrode is not activated or the activating time is insufficient Activate the electrode first - 26 - 6.5 Slope abnormal Cause Too many organic deposits on the Solution Wash it with deproteinised Solution. electrode membrane Reagent contaminated Replace the reagent Insufficient filling solution Refill the filling solution Electrode does not work Replace the failed electrode Dust or moisture around the electrode or plug Clean and dry the electrode and plug tubing loose reconnected Pump tube stuck recover the tube Pump tube broken replace the tube Tubing blockage clean the tubing Gasket between the electrodes wrong placed or missing Re assemble it Leakage between the electrode Reinstalled firmly Optical coupler too dirty or broken Clean or replace it Mixing Chamber Blocked Clean Mixing Chamber 6.6 AB slope and result abnormal Cause Solution condenser condensing Liquid outlet blocked AB slope is zero No Std R, pressure sensor cable loose or damaged. Mixer don’t move Reconnect J111 , replace mixing motor Liquid outlet valve don’t work Reconnect J111 , replace liquid outlet valve Air outlet don’t work, leakage, AB slope is low Reconnect J109, replace air outlet valve AB slope abnormal Std R invalid, leaked or blocked 6.7 Auto sampler trouble shooting. Cause Solution Auto Sampler can not be detected Check J112 connector and cable, replace Auto sampler motor The initial position of auto sampler is not on the clean position Check J103 connectors, clean the dust on the optical coupler for auto sampler home position, replace the optical coupler - 27 - Auto sampler on incorrect position Check J104 connector and cable, clean the dust on the counting optical coupler, replace the optical coupler 6.8 Aspiration abnormal Cause Solution Aspirating tube loose or broken Connect again or replace it Pump tube sticks Restore the tube Pump tube broken Replace the tube Pump tube blocked Clear the blockage The gasket between the electrodes does not placed properly or missing Place the gasket properly The electrode assembly leaks Tighten the assembly again Dust in the positioner, or positioner damaged Clean or replace the positioner - 28 - [...]... solutions please refer to “Trouble-shooting” -7- Chapter3 Functional description and operation 3.1 Flow chart of operation Please refer to the operator’s manual 3.2 System functions Please refer to the operator’s manual 3.3 Extended programs: Press Service in the main menu, press Time and enter “983”, press Exit, then the system will enter into extended program menu, the screen displays: (Attention:... check the positioner’s voltage with “Voltage” program in Service menu Rotate the pump manually and let the liquid pass through the positioner The positioner’s voltage should become small when there is liquid or big when there is no liquid The difference should be more than 500 Electrode: Check the voltage of the electrode by “Voltage” program in Service menu If the voltage is low, then the electrode... finish, the data will be automatically sent out during the time of printing The content of the transmitting data is almost same as the printing data The operator can also send out the data manually by “Send” program in Service menu Example: 001 111111 063 5.23 151.2 111.4 1.44 7.62 12.0 004 112224 063 5.25 151.6 110.8 1.40 7.59 42.0 Data format: Sample number + 2 blank + ID + 2 blank + symbol byte +... wash block is blocked, lift up the sample probe, remove the cover of the wash block, take out the rubber and clean it 4.6 Electrode maintenance and replacement 4.6.1 Structure of the electrode (For Erba lyte plus there will not be Ca & PH electrode) 4.6.2 Replacement of internal electrode and filling solution Follow the steps below to replace the internal electrode or filling solution: 1 Pull out all... the samples should be within 6-9, otherwise it will interfere in the measurement of sodium ions 9 Discard the reagent if mild dew or deposition found 10 Perform the routine maintenance according to the manual 11 Every electrode has a serial number Keep it on the electrode The warranty of the electrodes will become invalid if the number is not mentioned 12 When removing the sample tube after aspiration,... calibration or sample measurement, otherwise the result will be affected 14 Check and make sure the grounding is good if static interference is found 3.4.4 Daily operation Please refer to the operator’s manual - 12 - Chapter 4 Routine maintenance 4.1 Daily maintenance Keep the instrument clean, especially the sampling unit 4.2 Weekly maintenance 1 Check the electrodes and make sure the filling solutions... less than 2/3 2 Check if there is salty crystal on the internal electrode, if so, clean it with wet tampon 3 Check and clean the rubber in the wash block Clean the sample probe 4 Run deprotein program in service menu (If less than 5 samples per day, then just run deprotein program every 2 weeks) Check and clean the tubing system 5 Run “Na adjust” program if the slope of Na electrode is less than 50 4.3 ... operation manual and service manual carefully before operating When receiving the goods, please check the product model and spare parts against the packing list If missing or mistake, please contact Erba. .. chart of operation Please refer to the operator’s manual 3.2 System functions Please refer to the operator’s manual 3.3 Extended programs: Press Service in the main menu, press Time and enter “983”,...Company Contact Details: ERBA MANNHEIM Mallaustrasse 69-73 68219 Mannheim Germany Tel : (49-6-21) 8799770 Fax : (49-6-21) 8799688 Preface In order to operate and service the instrument properly,