Báo cáo y học: "Peripheral primitive neuroectodermal tumor of the urinary bladder in an Arab woman with history of squamous cell carcinoma: a case report" doc

Case report Open Access Peripheral primitive neuroectodermal tumor of the urinary bladder in an Arab woman with history of squamous cell carcinoma: a case report Mohd Khaled Al Meshaan 1 , Marwan Nayef 1 , Talal Kwaider 1 , Wolfgang Otto 2 * and Ken C. Katchy 3 Address: 1 Department of Urology, Sabah Hospital, Kuwait P.O. Box 3360, Safat 14013, State of Kuwait, 2 Department of Urology of Regensburg University, St. Josef’s Hospital, Landshuterstraße 65, 93053 Regensburg, Germany and 3 Department of Pathology, Sabah Hospital, Kuwait P.O. Box 3360, Safat 14013, State of Kuwait Email: MKA - dram_52@yahoo.com; MN - dr.marwan-nayef@hotmail.com; TK - talal_kwaider@yahoo.com; WO* - wolfgang1.otto@klinik.uni-regensburg.de; KK - kenkatchy@hotmail.com * Corresponding author Published: 29 April 2009 Received: 25 January 2008 Accepted: 2 February 2009 Journal of Medical Case Reports 2009, 3:6840 doi: 10.1186/1752-1947-3-6840 This article is available from: http://jmedicalcasereports.com/jmedicalcasereports/article/view/3/4/6840 © 2009 Al Meshaan et al; licensee Cases Network Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Introduction: Peripheral primitive neuroectodermal tumors of the urinary bladder are rare and tend to occur in an older age group than do their counterparts in bones and soft tissue. Case presentation: We report a case of peripheral primitive neuroectodermal tumor of the urinary bladder in a 67-year-old woman of Arab origin. She had undergone transurethral resection followed by chemotherapy because of pulmonary metastasized muscle-invasive squamous cell carcinoma of the bladder in 2005. One year later, she first presented with a history of repeated hematuria in our institution. Performing cystoscopy any tumor could be detected. Control cystoscopy two months later showed a tumor mass of 3 cm in diameter at another location than described for the first tumor. After perforating by transurethral resection partial bladder resection had to be done. Tissue specimen after pathological analysis revealed a peripheral primitive neuroectodermal tumor with tumor cells reactive to cluster of differentiation 99, neuron-specific enolase and S100 protein and stained negative for other markers such as cytokeratins, epithelial membrane antigen, desmin, smooth muscle actin, chromogranin and leucocyte common antigen. Staging computerized tomography was especially free from any hint on organ metastasis, but the patient died due to a cardiac problem only a few months later. Conclusions: To the best of our knowledge, we report the eighth case of bladder peripheral primitive neuroectodermal tumors in literature and the first concerning an Arab patient. It is also the first presentation of a peripheral primitive neuroectodermal tumor patient with a history of squamous cell carcinoma of the bladder. As in other cases, expression of single-chain-type 1 glycoprotein and neural markers was positive and the disease was at an advanced stage at the time of diagnosis. Page 1 of 4 (page number not for citation purposes) Introduction Peripheral primitive neuroectodermal tumors (pPNET) are malignant small round-cell tumors that occur predomi- nantly in bones and soft tissue of children and young adults [1], only in rare cases in other organs and in older patients [2]. pPNET belong to the Ewing’s sarcoma family of tumors and share the same immunohistochemical profiles and molecular genotypes [3]. Tumor cell staining of these masses was shown to be positive for MIC2 gene product (CD99) and neural markers such as neuron- specific enolase (NSE), vimentin, S100 protein or synap- tophysin [3–6]. Primitive neuroectodermal tumors of the urinary tract are very rare, more often occurring in renal masses than as bladder tumors [3]. To our knowledge, only 7 cases of primary pPNET of the urinary bladder have been reported so far [3–9], often concerning much older patients than those presenting with neuroectodermal tumors of other organs [5–7]. Poor prognosis necessitates multimodal treatment with chemotherapy and radio- therapy, following surgical measures [3]. Case presentation We report the first case of a peripheral primitive neuroectodermal tumor of the urinary bladder in an Arab country. The Department of Surgery of Al Sabah Hospital, Kuwait admitted a 67-year-old diabetic and hypertensive Arab woman in February 2006, due to severe hematuria and fever. In January 2005, the woman had already been treated in a hospital of Great Britain for repeated hematuria for the duration of one year. During this initial admission, cystoscopy revealed an intrinsic urinary bladder mass that was resected. Histologically, it was reported as poorly differentiated squamous cell carcinoma (pT 2 G 3 ). Computerized tomography (CT) scan showed diffuse but irregular thickening of the bladder wall, pelvic lymphadenopathy and multiple small bilat- eral pulmonary metastases. Mild hydronephrosis was noted in the right kidney, other abdominal organs were essentially normal. The patient received three courses chemotherapy, to which her response was excellent, and was discharged home in good condition. In November 2005, the patient underwent a follow-up cystoscopy that showed a recurrent malignant urinary bladder tumor which was completely resected. A CT scan of thorax, abdomen and pelvis, that did not show evidence of cancer progression, and a negative bone scan completed the follow-up. On first admission at our institution in February 2006, the patient was pale and pyretic. Her hemoglobin was 7.7 g/dl and the blood culture show ed an E. coli infection. Ultrasonography revealed hydronephrosis on the right side and a thick bladder wall without apparent masses, verified by cystoscopy. Based on the sensitivity report, she was treated with ciprofloxacin and a 3-way irrigation catheter was inserted to rinse the bladder. Although her general condition improved, mild hematuria persisted. Follow-up cystoscopy in April 2006 showed a tumor located at the posterior wall and the dome of the urinary bladder (another site than described for the squamous cell carcinoma). As an initial attempt at transurethral resection (TUR) resulted in perforation, a partial bladder resection was performed. A post-surgery CT of the thorax, abdomen and pelvis showed mild dilatation of the right renal pelvis, a thickened urinary bladder wall without an exophytic mass and no evidence of lung metastasis. The tumor specimen gained during surgery consisted of grey-brown tissue measuring 3 × 2.5 × 1 cm. Pathohistology showed features of a malignant small round-cell tumor (Figures 1 and 2), with frequent mitosis, apoptosis, necrosis, vascular invasion and deep infiltration of the muscularis propria. The tumor cells expressed CD99 (MIC2 gene, Figure 3), NSE and S100 protein, but were non-reactive to cytoker- atins, epithelial membr ane antigen, desm in, smooth muscle actin, chromogranin and leucocyte common antigen. A diagnosis of peripheral primitive neuroecto- dermal tumor (pPNET) obviously due to secondary tumor Figure 1. Malignant small round-cells arranged in irregular masses and clusters. Tumor is seen above the muscularis propria of the bladder wall in this field (HE, 40x). Page 2 of 4 (page number not for citation purposes) Journal of Medical Case Reports 2009, 3:6840 http://jmedicalcasereports.com/jmedicalcasereports/article/view/3/4/6840 development was made. The patient was discharged in good condition but died of cardiac-related problems in October 2006. Discussion and conclusions Peripheral primitive neuroectodermal tumors (pPNET) of the urinary bladder are rare, aggressive masses that partly tend to occur at a much older age than do their bone and soft tissue counterparts in the Ewing’s sarcoma family. It is said, that concerned patients are either of young age like in other small round-cell tumors [4, 8, 9] or of advanced age [5–7] as in our case. In this age group, these tumors may be confused with the more frequent transitional cell carcino- mas. Besides, pPNET must be differentiated from other rare tumors of the urinary bladder, such as embryonal rhabdomyosarcoma that may also occur in older age groups [10]. However, pPNET following or preceeding squamous cell carcinoma as in our case presentation was never reported before. Immunohistochemistry and, where available, molecular biology, are considered necessary in the evaluation of undifferentiated or poorly differentiated bladder tumors. In the present case, the immunohisto- chemistry profile supported the diagnosis of pPNET. As in most reported cases of pPNET, our patient showed positivity for neuron-specific enolase and MIC2 gene [3, 5, 6, 8]. Other neural markers showed different results in pPNET diagnostics. Whereas S100 protein was positive in our case, this marker showed different results in other pPNET patients with positive [3, 4, 6] and negative staining [8, 9]. Cytokeratins, epithelial membrane antigen, desmin (CD33), smooth muscle actin, chromogranin and leucocyte common antigen (CD3 und CD20) were not significant markers in this case, in accordance with the results of Desai and Banerjee et al [8,9]. Patients in some cases already had metastases at the time of diagnosis and this suggested a poor prognosis [5–8], however, it must be noted that the outcome of pPNET of the urinary bladder cannot be predicted. On the one hand, this is due to its rarity and thus missing long-term data, and, on the other hand, because it often affects older people with concomitant diseases, which may render differentiation of cause of death difficult. While our patient died soon after discharge of cardiac-related problems after a symptomatic period of 30 months of bladder tumor disease (squamous cell carcinoma and pPNET) in total, disease-free survival durations of up to 3 years have been reported [4]. List of abbreviations CD, Cluster of differentiation; CT, Computerized tomo- graphy; MIC2, Single-chain type-1 glycoprotein; NSE, Neuron-speci fic enolase; pPNET, Peripheral primiti ve neuroectodermal tumor; TUR, Transurethral resection. Consent Written informed consent was obtained from deceased patient relatives for publication of this case report and accompanying figures. A copy of the written consent is available for review by the Editor-in-Chief of this journal. Competing interests The authors declare that they have no competing interests. Figure 2. Predominantly small cells with round vesicular nuclei arranged in diffuse sheet (HE, 200x). Figure 3. The small round-cells of the pPNET in our case showed positivity for MIC2 gene (CD99+) (400x). Page 3 of 4 (page number not for citation purposes) Journal of Medical Case Reports 2009, 3:6840 http://jmedicalcasereports.com/jmedicalcasereports/article/view/3/4/6840 Authors’ contributions MKA supervised treatment of the patient and drafted the manuscript, MN, TK and WO helped to draft the manu- script. KK performed the histopathological analysis and helped to draft the manuscript. Acknowledgments The authors thank Prof. Dr. Wolf F. Wieland and PD Dr. Maximilian Burger, Department of Urology, University of Regensburg, Germany, for their excellent support. References 1. Katchy KC, Ziad F, Alexander S, Gad H, Abdel Mota’al M: Malignant bone tumors in Kuwait. A 10-year clinicopathological study. Int Orthop 2005, 29:406-411. 2. Kempson RL, Fletcher CDM, Evans HL, Hendrickson MR, Sibley RK: Peripheral Primitive Neuroectodermal Tumor/Ewing’s Sar- coma Family of Tumors. In Tumors of the Soft Tissues. Edited by Rosai J. Washington, D. C.: Armed Forces Institute of Pathology; 2001:444-452. 3. Ellinger J, Bastian PJ, Hauser S, Biermann K, Muller SC: Primitive neuroectodermal tumor: rare, highly aggressive differential diagnosis in urologic malignancies. Urology 2006, 68:257-62. 4. Lopez-Beltran A, Perez-Seoane C, Montironi R, Hernandez-Iglesias T, Mackintosh C, de Alava E: Primary primitive neuroectodermal tumor of the urinary bladder: a clinico-pathological study emphasizing immunohistochemical, ultrastructural and molecular analyses. J Clin Pathol. 2006, 59:775-778. 5. Kruger S, Schmidt H, Kausch I, Bohle A, Holzhausen HJ, Johannisson R, Feller AC: Primitive neuroectodermal tumor (PNET) of the urinary bladder. Pathol Res Pract 2003, 199:751-754. 6. Mentzel T, Flaschka J, Mentzel HJ, Eschholz G, Katenkamp D: Primary primitive neuroectodermal tumor of the urinary bladder. Clinicopathologic case report and differential small cell tumor diagnosis of this site. Pathologe 1998, 19:154-158. 7. Colecchia M, Dagrada GP, Poliani PI, Pilotti S: Immunophenotypic and genotypic analysis of a case of primary peripheral neuroectodermal tumour (pPNET) of the urinary bladder. Histopathology 2002, 40:108-109. 8. Desai S: Primary primitive neuroectodermal tumour of the urinary bladder. Histopathology 1998, 12:477-478. 9. Banerjee SS, Eyden BP, McVey RJ, Bryden AA, Clarke NW: Primary peripheral primitive neuroectodermal tumour of the urinary bladder. Histopathology 1997, 30:486-490. 10. Miettinen M: Rhabdomyosarcoma in patients older than 40 years of age. Cancer 1998, 62:2060-2065. Page 4 of 4 (page number not for citation purposes) Journal of Medical Case Reports 2009, 3:6840 http://jmedicalcasereports.com/jmedicalcasereports/article/view/3/4/6840 Do you have a case to share? Submit your case report today • Rapid peer review • Fast publication • PubMed indexing • Inclusion in Cases Database Any patient, any case, can teach us something www.casesnetwork.com . Case report Open Access Peripheral primitive neuroectodermal tumor of the urinary bladder in an Arab woman with history of squamous cell carcinoma: a case report Mohd Khaled Al Meshaan 1 , Marwan. admitted a 67-year-old diabetic and hypertensive Arab woman in February 2006, due to severe hematuria and fever. In January 2005, the woman had already been treated in a hospital of Great Britain for repeated. [3]. Case presentation We report the first case of a peripheral primitive neuroectodermal tumor of the urinary bladder in an Arab country. The Department of Surgery of Al Sabah Hospital, Kuwait admitted