báo cáo khoa học: " Characterization of cp3 reveals a new bri1 allele, bri1-120, and the importance of the LRR domain of BRI1 mediating BR signaling" doc
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Cấu trúc
Abstract
Background
Results
Conclusions
Background
Results
Phenotypic analyses of the weak bri1-looking semi-dwarf mutant, cp3
Identification of the weak bri1 mutant allele, bri1-120
BRI1(S399F) protein is localized in plasma membrane and the overexpression of BRI1(S399F) in bri1-301 resulted in the leaf elongation of bri1-301 and co-suppression of the endogenous bri1-301
Bri1-301 and bri1-120 complemented each other to form a functional BRI1 receptor
Different BL sensitivity was observed in the bri1-301 transformed with BRI1:bri1-120-GFP and the bri1-301 crossed with bri1-120
Discussions
BRI1-120 revealed the importance of the LRR region in the extracellular domain of BRI1
Interallelic complemented bri1-301 showed different BL sensitivity as compared to the bri1-301 overexpressing a BRI1:bri1-120-GFP
Conclusions
Methods
Plant growth condition
Construction of plasmids
Confocal microscopic analysis of the subcellular localization of BRI1(S399F)
Root growth inhibition assay
CPD expression analysis
Confirmation of BRI1 expression by RT-PCR analysis
Genotyping of bri1-120 and bri1-301 mutations
Detection of BRI1 proteins by western blot analysis