Atomic Force Microscopy in Cell Biology Episode 1 Part 1 pdf

[...]... of Linz, A-4040 Linz, Austria J K Heinrich Horber (1) , Cell Biology and Biophysics Program, European Molecular ¨ Biology Laboratory, D-6 911 7 Heidelberg, Germany Mike Horton (17 1), Bone and Mineral Center, Department of Medicine, The Rayne Institute, University College London, London WC1E 6JJ, United Kingdom A D L Humphris (337), H H Wills Physics Laboratory, University of Bristol, Bristol BS8 1TL,... Tübingen, Germany Matthias G Langer (14 1), Division of Sensory Biophysics, Universität Tübingen, D-72076 Tübingen, Germany* Petri Lehenkari (17 1), Departments of Surgery and Anatomy, University of Oulu, FIN90 014 Oulu, Finland P.-F Lenne ( 311 ), Cell Biology and Biophysics Program, European Molecular Biology Laboratory, D-6 911 7 Heidelberg, Germany Eric Lesniewska ( 51) , Laboratory of Physics, National...This Page Intentionally Left Blank CONTRIBUTORS Numbers in parentheses indicate the pages on which authors’ contributions begin S M Altmann ( 311 ), Cell Biology and Biophysics Program, European Molecular Biology Laboratory, D-6 911 7 Heidelberg, Germany Nathan Becker ( 213 ), Department of Physics, University of California, Santa Barbara, Santa Barbara, California 9 310 6 Martin Benoit ( 91) , Center for... Scanning Tunneling Microscopy III Atomic Force Microscopy A Combination with Optical Microscopy B Combination with Patch-Clamp Technique IV Force Spectroscopy A Molecular Adhesion B Intramolecular Forces C Combination with Optical and Patch-Clamp Techniques V Photonic Force Microscopy A Mechanics of Molecular Motors B Local Viscosity Measurements References I Introduction About 400 years ago, the invention... the Nobel Prize in Physics was awarded to Binnig and Rohrer in 19 86 In 19 86, Binnig together with Quate and Gerber demonstrated that the short-range van der Waals interaction can also be used to build a scanning probe microscope (Binnig et al., 19 86) This new device was called the atomic force microscope (AFM) With no electron transport involved, even insulators could be studied down to atomic resolution... Charras (17 1), Bone and Mineral Center, Department of Medicine, The Rayne Institute, University College London, London WC1E 6JJ, United Kingdom Aileen Chen (3 01) , Department of Physiology and Biophysics, University of Miami School of Medicine, Miami, Florida 3 313 6 Sang-Joon Cho (33), Department of Physiology and Pharmacology, Wayne State University School of Medicine, Detroit, Michigan 482 01 Daniel... Miami, Florida 3 313 6 Daniel J Muller (257), Max-Planck-Institute of Molecular Cell Biology and Genetics, ¨ D- 010 97 Dresden, Germany Emin Oroudjev ( 213 ), Department of Physics, University of California, Santa Barbara, Santa Barbara, California 9 310 6 Lia I Pietrasanta ( 213 ), Department of Physics, University of California, Santa Barbara, Santa Barbara, California 9 310 6 Arnd Pralle (19 3), Cell Biology and... 4 012 6 Bologna, Italy * Present address: HNO-Klinik, D-72076 T¨ bingen, Germany u address: Department of Molecular Cell Biology, University of California, Berkeley, Berkeley, California 94720 ‡ Present address: Department 1, Universit t Bremen, D-28359 Bremen, Germany † Present PREFACE In the last decade, the atomic force microscope (AFM) has emerged as a powerful tool for cell biology research giving... (2 31) , Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, Charlottesville, Virginia 22908 Andreas Engel (257), M E Müller Institute, Biocenter, University of Basel, CH-4056 Basel, Switzerland Ernst-Ludwig Florin (19 3), Cell Biology and Biophysics Program, European Molecular Biology Laboratory, D-6 911 7 Heidelberg, Germany Marie-C´ cile Giocondi ( 51) ,... BS8 1TL, United Kingdom Bhanu P Jena (33), Department of Physiology and Pharmacology, Wayne State University School of Medicine, Detroit, Michigan 482 01 Johannes H Kindt ( 213 ), Department of Physics, University of California, Santa Barbara, Santa Barbara, California 9 310 6 xi xii Contributors Assen Koitschev (14 1), Department of Otorhinolaryngology, Universität Tübingen, D-72076 Tübingen, Germany Matthias . 94 IV. Cell Culture 10 9 V. Final Remarks 11 0 References 11 1 6. Molecular Recognition Studies Using the Atomic Force Microscope Peter Hinterdorfer I. Introduction 11 5 II. Experimental Approach 11 7 III xiii 1. Local Probe Techniques J. K. Heinrich H ¨ orber I. Introduction 1 II. Scanning Tunneling Microscopy 4 III. Atomic Force Microscopy 7 IV. Force Spectroscopy 13 V. Photonic Force Microscopy. ¨orber EMBL Meyerhofstrasse 1 6 911 7 Heidelberg, Germany I. Introduction II. Scanning Tunneling Microscopy III. Atomic Force Microscopy A. Combination with Optical Microscopy B. Combination with Patch-Clamp