Atomic Force Microscopy in Cell Biology Episode 1 Part 1 pdf

... µg/mL and 1 mg/mL. After adsorption the AFM of Protein Complexes 217 217 16 Atomic Force Microscopy of Protein Complexes Olga I. Kiselyova and Igor V. Yaminsky 1. Introduction Scanning probe microscopy ... 72, 18 9R 19 6R. 5. Nagao, E. and Dvorak, J. A. (19 98) An integrated approach to the study of living cells by atomic force microscopy. J. Microsc. 19 1, 8 19 . 6. Radm...

Ngày tải lên: 06/08/2014, 02:20

... Wehrle-Haller, B., and Imhof, B. A. (19 98). Actin dynamics in living mammalian cells. J. Cell Sci. 11 1, 16 49 16 58. Barbee, K. A., Mundel, T., Lal, R., and Davies, P. F. (19 95). Subcellular distribution of ... Struct. Biol. 12 0, 18 0 19 1. Lehenkari, P. P., Charras, G. T., and Horton, M. A. (19 99). New technologies in scanning probe microscopy for the understanding of molecu...

Ngày tải lên: 06/08/2014, 02:20

... by atomic force microscopy. FEBS Lett. 3 81, 16 1 16 4. Mou, J., Sheng, S., Ho, R., and Shao, Z. (19 96). Chaperonins GroEL and GroES: views form atomic force microscopy. Biophys. J. 71, 2 213 –22 21. Mou, ... Biophys. J. 72, 13 08 13 18. Zhang, Y., Sheng, S., and Shao, Z. (19 96). Imaging biological structures with the cryo atomic force microscope. Biophys. J. 71, 216 8– 217...

Ngày tải lên: 06/08/2014, 02:20

... resonance. 15 . Forced Unfolding of Single Proteins 333 Fig. 17 The B–S transition of λ-phage DNA measured by AFM. Fig. 18 Monte Carlo simulations of spectrin extension. 15 . Forced Unfolding of Single ... of the instrument permitting). In the future, we expect an increasing contribution by forced unfolding measurements to the understanding of protein folding as, on the one hand, protei...

Ngày tải lên: 06/08/2014, 02:20

... with atomic force microscopy operating in different modes. Ultramicroscopy 68, 12 1 12 8. 15 . Vater, W., Fritzsche, W., Schaper, A., Bohm, K. J., Unger, E., and Jovin, T. M. (19 95) Scanning force microscopy ... 257, 19 00 19 05. 13 . Zasadzinski, J. A. N., and Hansma, P. K. (19 90) Scanning tunneling microscopy and atomic force microscopy of biological surfaces. Ann...

Ngày tải lên: 06/08/2014, 02:20

... Small Resolution 10 0 nm 5 nm 0.7 nm 0 .1 1. 0 nm x, y, z n/a n/a n/a 0. 01 nm Magnification 1x–2 × 10 3 x 10 x 10 6 x 9x 10 5 x5 × 10 2 x 10 8 x Necessary sample Low Critical point Critical point Low preparation ... (19 94) An atomic force microscope for cytological and histological investigations. J. Microsc. 17 6, 12 1 13 1. 12 . Ushiki, T., Hitomi, J., Ogura, S., Umemoto,...

Ngày tải lên: 06/08/2014, 02:20

... determined for each conjugation. A suggested range of dilutions is 1: 10 to 1: 100. 3. Wash cover slips in PBS (4 × 5 min) at 4°C and then fix cells for 15 min in 0.25% glutaraldehyde in 0 .1 M sodium ... (19 98) Atomic force microscopy on living cells: aldosterone-induced localized cell swelling. Kidney Blood Press. Res. 21, 256–258. 12 . Cines, D. B., Pollak, E. S. , Buc...

Ngày tải lên: 06/08/2014, 02:20

... Cells for live imaging are washed again in PBS containing 10 mg/ml FSG. The scanning experiments are carried out in culture medium for living cells and in PBS for fixed cells. In both cases, 10 ... Geerts, H. (19 91) . Lateral diffusion and retrograde movements of individual cell surface components on single motile cells observed with nanovid microscopy. J. Cell Biol. 11 2, 11...

Ngày tải lên: 06/08/2014, 02:20

... 200 nL/min, resulting in volume changes inside the fluid cell of less than ±6 μL in 1/ 2 h. A. Principle of Operation Two syringe pumps inject fluid into the AFM fluid cell in an alternating manner, ... minerals: characterization by atomic force microscopy. Langmuir 11 , 655–659. Brown, H. G., and Hoh, J. H. (19 97). Entropic exclusion by neurofilament sidearms: a mechanism for m...

Ngày tải lên: 06/08/2014, 02:20

... of the surrounding protein structure (Fig. 11 A; compare to Fig. 10 B). Increasing the force to approximately 15 0 pN further pushed the EF loop away (Fig. 11 B), whereas at about 18 0 pN the conformational change ... native 13 . Atomic Force Microscopy and Spectroscopy of Membrane Proteins 2 71 Fig. 8 Contact mode AFM topograph of α-hemolysin oligomers. The α-hemolysin inserte...

Ngày tải lên: 06/08/2014, 02:20