Deepshikha - CEMI Presentation pps

Presented By: Deepshikha Amity University Noida Objective  Optimization of process parameters to find out the best nanostructured conducting polyaniline in terms of conductivity and size.  Application of nanostructured conducting polyaniline to biosensors. Conducting Polymer A conductive polymer is an organic polymer semiconductor. They provide pathways for electronic conduction by doping. Common classes of organic conductive polymers include Poly(acetylene)s, Poly(pyrrole)s, Poly (thiophene)s, Poly(aniline)s etc. Characteristics of Nanostructured materials  Nanostructures are inorganic, organic materials with one dimension down to the nanometer range i.e. within 1-100 nm.  Unique aspect of nanomaterials is the vastly increase ratio of surface area to volume  Dramatic changes in properties (electrical, thermal, mechanical, electronic, optical) in nano scale dimension from their bulk material. Biosensor  Bio sensor is an analytical device which converts a biological response into readable signal .  Bio sensor comprises of three components: bioreceptor, transducer and detector. Advg of Nanostructured Conducting Polymers (NSCP) for Biosensor appl  High Surface Area  Unique optical, electronic and magnectic properties  Bio-compatibility  Dimensional compatibility with biomolecules.  Film forming ability.  Flexibility and processibility.  Size, stability, morphology , conductivity and porosity of nanostructured conducting polymers(NSCP) can be modified by changing reaction conditions.  Unique π electron uni-dimensional conductivity- enhancing the electron transfer rate(ETR) , lowering the detection potential and enhancing the stability of the enzyme .  Electrochemical switching depending upon pH and state of oxidation. Polymerisation procedure  Aniline was dissolved into SDA solution  Temperature was maintained at 0 0 to 5 0 C  Oxidizing agent, (NH4) 2 S 2 O 8 , in ice-cold water was added  Polymerisation was allowed to proceed for 3 to 4 h  Mixture was allowed to age under static condition for 1-3 days for complete polymerization. Effect of concentration of oxidizing agent on the properties of NSPANI  Smallest size, uniform size distribution and the highest conductivity are obtained when molar concentration of oxidizing agent is in the range of 0.01-0.03M. PANI1 PANI2 PANI3 PANI4 0 50 100 150 200 250 300 350 400 450 500 PANI1 PANI2 PANI3 PANI4 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0 1.1 Polydispersity Index(PDI) PANI1 PANI2 PANI3 PANI4 0 100 200 300 400 500 600 700 800 900 Z-Average(d-nm) 400 600 800 1000 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 803nm 806nm 789nm 778nm PANI1 PANI3 PANI2 PANI4 Absorbance Wavelength(nm) Resistance(Kilo ohms) Effect of concentration of monomer on the properties of NSPANI Smallest size, uniform size distribution and the highest conductivity are obtained when monomer concentration is in the range of 0.01-0.03M. PANI2 PANI8 10 15 20 25 30 35 40 45 50 PANI2 PANI8 0.42 0.44 0.46 0.48 0.50 0.52 0.54 0.56 0.58 0.60 0.62 Polydispersity Index(PDI) PANI2 PANI8 100 120 140 160 180 200 220 240 Z-Average(d-nm) 400 600 800 1000 0.0 0.5 1.0 1.5 2.0 2.5 3.0 806nm 790nm PANI8 PANI2 Absorbance Wavelength(nm) Resistance(Kilo ohms) Effect of concentration of structure directing agent on the properties of NSPANI Smallest size, uniform size distribution and the highest conductivity are obtained when SDS concentration is in the range of 0.06-0.08M. PANI5 PANI2 PANI6 PANI7 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 PANI5 PANI2 PANI6 PANI7 0.40 0.45 0.50 0.55 0.60 0.65 Polydispersity Index(PDI) PANI5 PANI2 PANI6 PANI7 100 120 140 160 180 200 220 240 Z-Average(d-nm) 400 600 800 1000 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 771nm 795nm 801nm 806nm PANI5 PANI7 PANI2 PANI6 Absorbance Wavelength(nm) Resistance (Kilo ohms) [...]... 0.00010 0.00005 b c 0.00000 -0 .00005 -0 .00010 -0 .00015 -0 .4 -0 .2 0.0 0.2 0.4 0.6 0.8 1.0 1.2 Potential(V) Pictorial representation of synthesis of NSPANI, Immobilization of enzyme and biochemical reaction at Enzyme/NSPANI/ITO bioelectrode ITO Electrode Film formation of NSPANI on ITO Polyaniline nanoparticles (NSPANI) Glutaraldehyde Enzyme Polymerization of aniline in the OHC-(CH2)3-CHO presence of soft... LB, respectively Small redox peak around +350 mV (C and C’) is associated with the formation of p-benzoquinone and hydroquinone as a side product Current(m icro am peres) Cyclic Voltammetric studies of best nanostructured conducting polyaniline B 0.00010 0.00005 C A 0.00000 B' -0 .00005 C' -0 .00010 A' -0 .4 -0 .2 0.0 0.2 0.4 0.6 Potential(V) 0.8 1.0 1.2 Application of nanostructured conducting polyaniline... 100 0.02 0.03 0.04 0.05 0.06 0.07 Conc of surfactant(m/l) 0.08 Optimum process conditions for nanostructured conducting polyaniline Monomer: 0.0 1-0 .03M Structure directing agent: 0.0 6-0 .08M Oxidant: 0.0 1-0 .03M Temperature: 0 0- 50 C Conditioning time: 1-3 days Cyclic voltammetry was the method used for electrodeposition of the NSPANI emeraldine salt (ES) onto ITO from the aqueous dispersion of nanoparticles... bioelectrode  Anodic current increases with Current(Amperes) increased concentration of glucose c 0.00015 b 0.00010 a 0.00005 0.00000 -0 .00005 -0 .00010 -0 .4 -0 .2 0.0 0.2 0.4 0.6 Potential(V) 0.8 1.0 1.2 Photometric response studies of H2O2 biosensor HRP/NSPANI H2O2 + O-anisidine (red) 1.0 0.9 0.8 0.7 Absorbance The value of absorbance resulting from the oxidized form of dye has been found to be increasing... Glucose+O2 0.40 CONH Gluconic acid+H2O2 H2O2+O-anisidine (Reduced) 0.45 CONH CONH O-anisidine (Oxidised) 0.35 0.30 0.25 0.20 a 0 10 20 30 40 Immobilization of enzyme 50 Conc(mM/l) on NSPANI film Photometric response study for the detection of analyte Sodium dodecyl sulphate Aniline Enzyme Photometric response studies of glucose biosensor GOx/NSPANI Glucose + O2 H2O2 + O-anisidine (red) Gluconic acid + H 2O2... Malhotra B D, Cholesterol biosensor based on electrophoretically deposited conducting polymer film derived from nano-structured polyaniline colloidal suspension Anal Chim Acta, 602 (2007) 244 Solanki P R, Kaushik A, Ansari A A, Tiwari A & Malhotra B D, Multi-walled carbon nanotubes/sol–gel-derived silica/chitosan nanobiocomposite for total cholesterol sensor, Sensors and Actuators B ,xxx (2009) xxx Dhand... has been found to be increasing linearly in the range of 1 mM/l to 50 mM/l for HRP/NSPANI where as the bulk PANI, exhibits linearity between 10 mM/L to 30 mM/L 0.6 0.5 0.4 2 H 2O + O-anisidine (oxidized) 0.3 0.2 (Orange-red colour) a 0.1 0.0 0 10 20 30 Conc(mM/l) 40 50 b Optical characteristics of various H2O2 biosensors Name of the Km(mM) Linearity(m Standard samples M/l) HRP/bulk Regression Deviation... absorbance resulting from the oxidized form of dye was found to be increasing linearly in the range of 5 mM/l to 40 mM/l for GoX/NSPANI where as bulk PANI exhibits linearity between 5-2 0mM 0.60 0.55 b 0.50 0.45 0.40 0.35 2 H 2O + O-anisidine 0.30 (oxidized) 0.25 0.20 a 0 10 20 30 Conc(mM/l) 40 50 Optical characteristics of various glucose biosensors Name of the Km(mM) Linearity(m Response Lower bioelectrode... 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