Trichomonas HiVeg Agar Base with Serum and Selective Supplement 1815 Tributyrin HiVeg Agar Base with Tributyrin Composition per liter: Agar 15.0g Plant peptone 5.0g Yeast extract 3.0g Tributyrin (glyceryl tributyrate) 10.0mL pH 7.5 ± 0.2 at 25°C Source: This medium, without tributyrin, is available as a premixed powder from HiMedia. Preparation of Medium: Add components to distilled/deionized water and bring volume to 990.0mL. Add 10.0mL of tributyrin. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°– 50°C. Mix thoroughly. Pour into sterile Petri dishes or leave in tubes. Use: For the detection of lipolytic microorganisms. Trichlorophenol Medium Composition per liter: Pancreatic digest of casein 8.5g NaCl 2.5g Papaic digest of soybean meal 1.5g K 2 HPO 4 1.25g Glucose 1.25g 2,4,6-Trichlorophenol 1.25g pH 7.3 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat until dis- solved. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Use: For the cultivation and maintenance of Arthrobacter species and other microorganisms that can degrade chlorinated phenols. Trichococcus Medium Composition per liter: Pancreatic digest of casein 10.0g Na 2 SO 4 4.0g MgCl 2 ·6H 2 O 1.1g KCl 0.7g Glucose solution 30.0mL pH 7.3 ± 0.2 at 25°C Glucose Solution: Composition per 30.0mL: Glucose 3.0g Preparation of Glucose Solution: Add glucose to distilled/deion- ized water and bring volume to 30.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, except glucose solu- tion, to distilled/deionized water and bring volume to 970.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Aseptical- ly add 30.0mL of sterile glucose solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For the cultivation of Trichococcus flocculiformis. Trichomonas HiVeg Agar Base with Serum Composition per liter: Plant extract No. 2 25.0g NaCl 6.5g Glucose 5.0g Agar 1.0g Horse serum 80.0mL pH 6.4 ± 0.2 at 25°C Source: This medium, without horse serum, is available as a pre- mixed powder from HiMedia. Horse Serum: Composition per 80.0mL: Horse serum 80.0mL Preparation of Horse Serum: Gently heat sterile horse serum to 56°C for 30 min. Aseptically adjust pH to 6.0 with 0.1N HCl. Use immediately. Preparation of Medium: Add components, except horse serum, to distilled/deionized water and bring volume to 920.0mL. Mix thorough- ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 80.0mL of freshly prepared sterile horse serum. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For the cultivation of Trichomonas vaginalis. Trichomonas HiVeg Agar Base with Serum and Selective Supplement Composition per liter: Plant extract No. 2 25.0g NaCl 6.5g Glucose 5.0g Agar 1.0g Horse serum 80.0mL Selective supplement 10.0mL pH 6.4 ± 0.2 at 25°C Source: This medium, without horse serum or selective supplement, is available as a premixed powder from HiMedia. Horse Serum: Composition per 80.0mL: Horse serum 80.0mL Preparation of Horse Serum: Gently heat sterile horse serum to 56°C for 30 min. Aseptically adjust pH to 6.0 with 0.1N HCl. Use immediately. Selective Supplement: Composition per 10.0mL: Streptomycin 0.5g Penicllin 1,000,000U Preparation of Selective Supplement: Add components to dis- tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, except horse serum and selective supplement, to distilled/deionized water and bring vol- ume to 910.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 80.0mL of freshly prepared sterile horse serum and 10.0mL sterile selective supplement. Mix thoroughly. Aseptically dis- tribute into sterile tubes or flasks. Use: For the cultivation of Trichomonas vaginalis. © 2010 by Taylor and Francis Group, LLC 1816 Trichomonas Medium Trichomonas Medium Composition per liter: Liver digest 25.0g NaCl 6.5g Glucose 5.0g Agar 1.0g Horse serum 80.0mL pH 6.4 ± 0.2 at 25°C Source: This medium is available as a premixed powder from Oxoid Unipath. Horse Serum: Composition per 80.0mL: Horse serum 80.0mL Preparation of Horse Serum: Gently heat sterile horse serum to 56°C for 30 min. Aseptically adjust pH to 6.0 with 0.1N HCl. Use im- mediately. Preparation of Medium: Add components, except horse serum, to distilled/deionized water and bring volume to 920.0mL. Mix thorough- ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 80.0mL of freshly prepared sterile horse serum. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For the cultivation of Trichomonas vaginalis. Trichomonas Medium No. 2 Composition per liter: Glucose 22.5g Liver digest 18.0g Pancreatic digest of casein 17.0g NaCl 5.0g Pancreatic digest of soybean meal 3.0g K 2 HPO 4 2.5g Chloramphenicol 0.125g Horse serum 250.0mL Calcium pantothenate (0.5% solution) 1.0mL pH 6.2 ± 0.2 at 25°C Source: This medium is available as a prepared medium from Oxoid Un- ipath. Preparation of Medium: Add components, except horse serum, to distilled/deionized water and bring volume to 750.0mL. Mix thorough- ly. Autoclave for 15 min at 5 psi pressure–108°C. Cool to 45°–50°C. Aseptically add 250.0mL of sterile horse serum. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For the isolation of Trichomonas vaginalis. Trichomonas Selective Medium Composition per liter: Liver digest 25.0g NaCl 6.5g Glucose 5.0g Agar 1.0g Horse serum 80.0mL Antibiotic inhibitor 10.0mL pH 6.4 ± 0.2 at 25°C Source: This medium is available as a premixed powder from Oxoid Unipath. Horse Serum: Composition per 80.0mL: Horse serum 80.0mL Preparation of Horse Serum: Gently heat sterile horse serum to 56°C for 30 min. Aseptically adjust pH to 6.0 with 0.1N HCl. Use im- mediately. Antibiotic Inhibitor: Composition per 10.0mL: Streptomycin 500.0mg Penicillin G 1,000,000U Preparation of Antibiotic Inhibitor: Add components to dis- tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, except horse serum and antibiotic inhibitor, to distilled/deionized water and bring volume to 910.0mL. Mix thoroughly. Gently heat and bring to boiling. Auto- clave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Asepti- cally add 80.0mL of freshly prepared sterile horse serum and 10.0mL of sterile antibiotic inhibitor. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For the cultivation of Trichomonas vaginalis from specimens with a mixed bacterial flora. Trichomonas Selective Medium Composition per liter: Liver digest 25.0g NaCl 6.5g Glucose 5.0g Agar 1.0g Horse serum 80.0mL Antibiotic inhibitor 10.0mL pH 6.4 ± 0.2 at 25°C Horse Serum: Composition per 80.0mL: Horse serum 80.0mL Preparation of Horse Serum: Gently heat sterile horse serum to 56°C for 30 min. Aseptically adjust pH to 6.0 with 0.1N HCl. Use im- mediately. Antibiotic Inhibitor: Composition per 10.0mL: Chloramphenicol 100.0mg Preparation of Antibiotic Inhibitor: Add chloramphenicol to dis- tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, except horse serum and antibiotic inhibitor, to distilled/deionized water and bring volume to 910.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 80.0mL of freshly prepared sterile horse serum and 10.0mL of sterile an- tibiotic inhibitor. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For the cultivation of Trichomonas vaginalis from specimens with a mixed bacterial flora. Trichophyton Agar 1 Composition per liter: Glucose 40.0g Agar 15.0g © 2010 by Taylor and Francis Group, LLC Trichophyton Agar 7 1817 Vitamin assay casamino acids 2.5g KH 2 PO 4 1.8g MgSO 4 ·7H 2 O 0.1g pH 6.8 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes. Autoclave for 15 min at 15 psi pres- sure–121°C. Allow tubes to cool in a slanted position. Use: For the differentiation of the Trichophyton species. Trichophyton Agar 2 Composition per liter: Glucose 40.0g Agar 15.0g Vitamin assay casamino acids 2.5g KH 2 PO 4 1.8g MgSO 4 ·7H 2 O 0.1g Inositol 50.0mg pH 6.8 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes. Autoclave for 15 min at 15 psi pres- sure–121°C. Allow tubes to cool in a slanted position. Use: For the differentiation of the Trichophyton species. Trichophyton Agar 3 Composition per liter: Glucose 40.0g Agar 15.0g Vitamin assay casamino acids 2.5g KH 2 PO 4 1.8g MgSO 4 ·7H 2 O 0.1g Inositol 0.05g Thiamine·HCl 0.2mg pH 6.8 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes. Autoclave for 15 min at 15 psi pres- sure–121°C. Allow tubes to cool in a slanted position. Use: For the differentiation of the Trichophyton species. Trichophyton Agar 4 Composition per liter: Glucose 40.0g Agar 15.0g Vitamin assay casamino acids 2.5g KH 2 PO 4 1.8g MgSO 4 ·7H 2 O 0.1g Thiamine·HCl USP 200.0μg pH 6.8 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes. Autoclave for 15 min at 15 psi pres- sure–121°C. Allow tubes to cool in a slanted position. Use: For the differentiation of the Trichophyton species. Trichophyton Agar 5 Composition per liter: Glucose 40.0g Agar 15.0g Vitamin assay casamino acids 2.5g KH 2 PO 4 1.8g MgSO 4 ·7H 2 O 0.1g Nicotinic acid 2.0mg pH 6.8 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes. Autoclave for 15 min at 15 psi pres- sure–121°C. Allow tubes to cool in a slanted position. Use: For the differentiation of the Trichophyton species. Trichophyton Agar 6 Composition per liter: Glucose 40.0g Agar 15.0g KH 2 PO 4 1.8g Ammonium nitrate 1.5g MgSO 4 ·7H 2 O 0.1g pH 6.8 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems. Preparation of Medium: Add components to distilled/deionized wa- ter and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes. Autoclave for 15 min at 15 psi pressure– 121°C. Allow tubes to cool in a slanted position. Use: For the differentiation of the Trichophyton species. Trichophyton Agar 7 Composition per liter: Glucose 40.0g Agar 15.0g KH 2 PO 4 1.8g Ammonium nitrate 1.5g MgSO 4 ·7H 2 O 0.1g Histidine·HCl 0.03g pH 6.8 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes. Autoclave for 15 min at 15 psi pres- sure–121°C. Allow tubes to cool in a slanted position. © 2010 by Taylor and Francis Group, LLC 1818 Trichophyton HiVeg Agar 1 Use: For the differentiation of the Trichophyton species. Trichophyton HiVeg Agar 1 Composition per liter: Glucose 40.0g Agar 15.0g Vitamin-free casein enzymic hydrolysate 2.5g KH 2 PO 4 1.8g MgSO 4 0.1g pH 6.8 ± 0.2 at 25°C Source: This medium is available as a premixed powder from Hi- Media. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes. Autoclave for 15 min at 15 psi pres- sure–121°C. Allow tubes to cool in a slanted position. Use: For the differentiation of the Trichophyton species. Trichophyton HiVeg Agar 2 Composition per liter: Glucose 40.0g Agar 15.0g Vitamin-free plant hydrolysate 2.5g KH 2 PO 4 1.8g MgSO 4 0.1g Inositol 5.0mg pH 6.8 ± 0.2 at 25°C Source: This medium is available as a premixed powder from Hi- Media. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes. Autoclave for 15 min at 15 psi pres- sure–121°C. Allow tubes to cool in a slanted position. Use: For the differentiation of the Trichophyton species. Trichophyton HiVeg Agar 3 Composition per liter: Glucose 40.0g Agar 15.0g Vitamin-free plant hydrolysate 2.5g KH 2 PO 4 1.8g MgSO 4 0.1g Inositol 5.0mg Thiamine 5.0mg pH 6.8 ± 0.2 at 25°C Source: This medium is available as a premixed powder from Hi- Media. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes. Autoclave for 15 min at 15 psi pres- sure–121°C. Allow tubes to cool in a slanted position. Use: For the differentiation of the Trichophyton species. Trichophyton HiVeg Agar 4 Composition per liter: Glucose 40.0g Agar 15.0g KH 2 PO 4 1.8g MgSO 4 0.1g Vitamin-free plant hydrolysate 2.5g Thiamine hydrochloride 0.2mg pH 6.8 ± 0.2 at 25°C Source: This medium is available as a premixed powder from Hi- Media. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes. Autoclave for 15 min at 15 psi pres- sure–121°C. Allow tubes to cool in a slanted position. Use: For the differentiation of the Trichophyton species. Trichophyton HiVeg Agar 5 Composition per liter: Glucose 40.0g Agar 15.0g Vitamin-free plant hydrolysate 2.5g KH 2 PO 4 1.8g MgSO 4 0.1g Nicotinic acid 0.02g pH 6.8 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes. Autoclave for 15 min at 15 psi pres- sure–121°C. Allow tubes to cool in a slanted position. Use: For the differentiation of the Trichophyton species. Trichosel™ Broth, Modified Composition per liter: Pancreatic digest of casein 12.0g Yeast extract 5.0g Liver extract 2.0g Maltose 2.0g L-Cysteine·HCl 1.0g Agar 1.0g Chloramphenicol 0.1g Methylene Blue 3.0mg Horse serum 50.0mL pH 6.0 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems. Preparation of Medium: Add components, except horse serum, to distilled/deionized water and bring volume to 950.0mL. Mix thorough- ly. Gently heat while stirring and bring to boiling. Autoclave for 15 min at 13 psi pressure–118°C. Cool to 45°–50°C. Aseptically add 50.0mL of sterile horse serum. Mix thoroughly. Aseptically distribute into ster- ile tubes or flasks. Use: For the isolation and cultivation of Trichomonas species. Trimethylamine N-Oxide Medium (TMAO Medium) Composition per liter: Beef extract 10.0g Peptone 10.0g NaCl 5.0g © 2010 by Taylor and Francis Group, LLC Triple Sugar Iron Agar, HiVeg 1819 Agar 2.0g Trimethylamine N-oxide 1.0g Yeast extract 1.0g pH 7.5 ± 0.2 at 25°C Source: This medium is available as a premixed powder from Oxoid Unipath. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into screw-capped tubes in 4.0mL vol- umes. Autoclave for 15 min at 15 psi pressure–121°C. Allow tubes to cool in an upright position. Use: For the cultivation and differentiation of Campylobacter species from foods. Campylobacter jejuni and Campylobacter coli will not grow. Triple Sugar Iron Agar (TSI Agar) Composition per liter: Peptone 20.0g Agar 12.0g Lactose 10.0g Sucrose 10.0g NaCl 5.0g Beef extract 3.0g Yeast extract 3.0g Glucose 1.0g Ferric citrate 0.3g Na 2 S 2 O 3 0.3g Phenol Red 0.025g pH 7.4 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems and Oxoid Unipath. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Allow tubes to cool in a slanted position to form a 1.0-inch butt. Use: For the differentiation of members of the Enterobacteriaceae based on their fermentation of lactose, sucrose, and glucose and the production of H 2 S. Triple Sugar Iron Agar (TSI Agar) Composition per liter: Agar 13.0g Pancreatic digest of casein 10.0g Peptic digest of animal tissue 10.0g Lactose 10.0g Sucrose 10.0g NaCl 5.0g Glucose 1.0g Fe(NH 4 ) 2 (SO 4 ) 2 ·6H 2 O 0.2g Na 2 S 2 O 3 0.2g Phenol Red 0.025g pH 7.3 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Allow tubes to cool in a slanted position to form a 1.0-inch butt. Use: For the differentiation of members of the Enterobacteriaceae based on their fermentation of lactose, sucrose, and glucose and the production of H 2 S. Triple Sugar Iron Agar (TSI Agar) (BAM M149 Medium 2) Composition per liter: Peptone 15.0g Agar 12.0g Lactose 10.0g Sucrose 10.0g Proteose peptone 5.0g NaCl 5.0g Beef extract 3.0g Yeast extract 3.0g Glucose 1.0g Na 2 S 2 O 3 0.3g FeSO 4 0.2g Phenol Red 0.024g pH 7.4 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Allow tubes to cool in a slanted position to form a 1.0-inch butt. Use: For the differentiation of members of the Enterobacteriaceae based on their fermentation of lactose, sucrose, and glucose and the production of H 2 S. Triple Sugar Iron Agar, HiVeg Composition per liter: Agar 12.0g Plant hydrolysate 10.0g Plant peptone 10.0g Lactose 10.0g Sucrose 10.0g NaCl 5.0g Plant extract 3.0g Yeast extract 3.0g Glucose 1.0g Na 2 S 2 O 3 0.3g FeSO 4 0.2g Phenol Red 0.024g pH 7.4 ± 0.2 at 25°C Source: This medium is available as a premixed powder from Hi- Media. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Allow tubes to cool in a slanted position to form a 1.0-inch butt. © 2010 by Taylor and Francis Group, LLC 1820 Tris YP Agar Use: For the differentiation of members of the Enterobacteriaceae based on their fermentation of lactose, sucrose, and glucose and the production of H 2 S. Tris YP Agar (Tris Yeast Extract Peptone Agar) Composition per liter: Agar 19.0g Yeast extract 3.0g Glucose 1.0g Peptone 0.6g Tris buffer (0.05M, pH 7.5) 1.0L pH 7.5 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. For top layer agar, add 6.0g of agar instead of 19.0g. Mix thoroughly. Gently heat and bring to boiling. Dis- tribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C. Pour into sterile Petri dishes. Use: For the cultivation and maintenance of Bdellovibrio species. Tris YP Broth (Tris Yeast Extract Peptone Broth) Composition per liter: Yeast extract 3.0g Glucose 1.0g Peptone 0.6g Tris buffer (0.05M, pH 7.5) 1.0L pH 7.5 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Use: For the cultivation and maintenance of Bdellovibrio species. Trypaflavin Nalidixic Acid Serum Agar (TNSA Agar) Composition per liter: Ionagar No. 2 12.0g Peptone 10.0g Beef extract 3.0g H 2 O 926.5mL Bovine serum, heat inactivated 50.0mL Nalidixic acid solution 20.0mL Trypaflavin solution 3.5mL pH 7.2–7.4 at 25°C Nalidixic Acid Solution: Composition per 10.0mL: Nalidixic acid 0.02g Preparation of Nalidixic Acid Solution: Add nalidixic acid to distilled/deionized water and bring volume to 10.0mL. Mix thorough- ly. Filter sterilize. Trypaflavin Solution: Composition per 10.0mL: Trypaflavin 0.1g Preparation of Trypaflavin Solution: Add trypaflavin to dis- tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components—except bovine serum, nalidixic acid solution, and trypaflavin solution—to distilled/deionized water and bring volume to 926.5mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 50.0mL of sterile bovine serum, 20.0mL of sterile nalidixic acid solution, and 3.5mL of trypaflavin solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the isolation and cultivation of Listeria species from preen- riched specimens. Trypanosome Medium Composition per 1300.0mL: Solid phase 1.0L Liquid phase (Locke’s solution) 300.0mL pH 7.2–7.4 at 25°C Solid Phase: Composition per liter: Agar 15.0g NaCl 8.0g Peptone 5.0g Beef extract 3.0g Rabbit blood, defibrinated 300.0mL Preparation of Solid Phase: Add components, except rabbit blood, to distilled/deionized water and bring volume to 700.0mL. Mix thoroughly. Adjust pH to 7.2–7.4. Gently heat and bring to boiling. Au- toclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Asep- tically add 300.0mL of sterile defibrinated rabbit blood. Mix thoroughly. Distribute 10.0mL aliquots into sterile screw-capped tubes. Allow to solidify in a slanted position. Liquid Phase (Locke’s Solution): Composition per liter: NaCl 8.0g Glucose 2.5g KH 2 PO 4 0.3g CaCl 2 0.2g KCl 0.2g Preparation of Liquid Phase (Locke’s Solution): Add compo- nents to distilled/deionized water and bring volume to 1.0L. Mix thor- oughly. Autoclave for 15 min at 15 psi pressure–121°C. Preparation of Medium: Aseptically overlay agar slants (solid phase) with 3.0mL per tube of sterile liquid phase (Locke’s solution). Use: For the cultivation of Leishmania donovani, Leishmania brazil- iensis, Trypanosoma gambiense, and Trypanosoma rhodesiense. Tryptic Digest Broth Composition per liter: Tryptic digest of beef heart 10.0g NaCl 5.0g Glucose 1.0g pH 7.6 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. © 2010 by Taylor and Francis Group, LLC Tryptic Soy Agar with 0.6% Yeast Extract 1821 Use: For use as a base medium to which enrichments are added. For the cultivation of fastidious microorganisms. Tryptic Nitrate Medium Composition per liter: Tryptose 20.0g Na 2 HPO 4 2.0g Agar 1.0g Glucose 1.0g KNO 3 1.0g pH 7.6 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes in 10.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C. Use: For the cultivation and differentiation of Pseudomonas and related genera. For the differentiation of bacteria based on their reduc- tion of nitrate to nitrite. After incubation of the bacterium in tryptic nitrate medium for 18–24 hr, sulfanillic acid and α-naphthol reagents are added. Nitrate reduction is indicated by the development of a red to violet color. Tryptic Soy Agar See: Trypticase™ Soy Agar Tryptic Soy Agar Blood Agar Base See: Trypticase™ Soy Agar with Sheep Blood Tryptic Soy Agar with Magnesium Ions Composition per liter: Pancreatic digest of casein 17.0g Agar 15.0g NaCl 5.0g Pancreatic digest of soybean meal 3.0g Glucose 2.5g K 2 HPO 4 2.5g MgCl 2 0.95g pH 7.3 ± 0.2 at 25°C Preparation of Medium: Add components to tap water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Dis- tribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation of Escherichia coli for bacteriophage produc- tion. Tryptic Soy Agar with Magnesium Sulfate Composition per liter: Agar 15.0g Pancreatic digest of casein 15.0g NaCl 5.0g Pancreatic digest of soybean meal 5.0g MgSO 4 ·7H 2 O 1.5g pH 7.3 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes in 20.0mL volumes. Use: For the cultivation of Escherichia coli from foods. Tryptic Soy Agar with Magnesium Sulfate and Sodium Chloride Composition per liter: Pancreatic digest of casein 50.0g NaCl 30.0g Agar 15.0g Pancreatic digest of soybean meal 5.0g MgSO 4 ·7H 2 O 1.5g pH 7.3 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes in 20.0mL volumes. Use: For the cultivation of Vibrio species from foods. Tryptic Soy Agar with Sodium Chloride (ATCC Medium 2276) Composition per liter: Pancreatic digest of casein 50.0g NaCl 20.0g Agar 15.0g Pancreatic digest of soybean meal 5.0g MgSO 4 ·7H 2 O 1.5g pH 7.3 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation of Vibrio species. Tryptic Soy Agar with 0.6% Yeast Extract Composition per liter: Agar 15.0g Pancreatic digest of casein 15.0g Yeast extract 6.0g Pancreatic digest of soybean meal 5.0g NaCl 5.0g pH 7.0–7.5 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the isolation and cultivation of Listeria monocytogenes from foods. Tryptic Soy Blood Agar See: Trypticase™ Soy Agar with Sheep Blood Tryptic Soy Blood Agar with VAN 4 See: Trypticase™ Soy Agar with Sheep Blood and Vancomycin © 2010 by Taylor and Francis Group, LLC 1822 Tryptic Soy Broth Tryptic Soy Broth Composition per liter: Pancreatic digest of casein 18.0g Papaic digest of soybean meal 6.0g NaCl 6.0g pH 7.3 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Mix thoroughly. Use: For the isolation and cultivation of a wide variety of microorgan- isms. Tryptic Soy Broth with 0.001M Calcium Chloride (ATCC Medium 1380) Composition per liter: Pancreatic digest of casein 18.0g Papaic digest of soybean meal 6.0g NaCl 6.0g CaCl 2 solution 10.0mL pH 7.3 ± 0.2 at 25°C Source: This medium, without CaCl 2 , is available as a premixed pow- der from BD Diagnostic Systems. Calcium Chloride Solution: Composition per 10.0mL: CaCl 2 0.111g Preparation of Calcium Chloride Solution: Add CaCl 2 to dis- tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, except CaCl 2 solution, to distilled/deionized water and bring volume to 990mL. Mix thor- oughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add 10.0mL CaCl 2 solution. Mix thor- oughly. Distribute into tubes or flasks. Use: For the isolation and cultivation of Bronchothrix thermospacta. Tryptic Soy Broth with 0.1% Potassium Nitrate (ATCC Medium 1183) Composition per liter: Pancreatic digest of casein 15.0g Agar 15.0g Papaic digest of soybean meal 5.0g NaCl 5.0g KNO 3 1.0g pH 7.3 ± 0.2 at 25°C Source: This medium, without nitrate, is available as a premixed pow- der from BD Diagnostic Systems. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Do not overheat. Pour into sterile Petri dishes or leave in tubes. Use: For the isolation and cultivation of Flavobacterium sp. Tryptic Soy Broth with Magnesium Ions (ATCC Medium 1588) Composition per liter: Pancreatic digest of casein 17.0g NaCl 5.0g Pancreatic digest of soybean meal 3.0g Glucose 2.5g K 2 HPO 4 2.5g MgCl 2 0.95g pH 7.3 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into flasks or tubes. Autoclave for 15 min at 15 psi pressure–121°C. Use: For the cultivation of Escherichia coli for bacteriophage produc- tion. Tryptic Soy Broth with 1M Potassium Chloride (ATCC Medium 2074) Composition per liter: KCl 74.5g Pancreatic digest of casein 17.0g NaCl 5.0g Papaic digest of soybean meal 3.0g K 2 HPO 4 2.5g Glucose 2.5g pH 7.3 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Use: For the cultivation and maintenance of Gracilibacillus dipso- sauri. Tryptic Soy Fast Green Agar (TSFA) Composition per liter: Pancreatic digest of casein 17.0g Agar 15.0g NaCl 5.0g Papaic digest of soybean meal 3.0g K 2 HPO 4 2.5g Glucose 2.5g Fast Green FCF 0.25g pH 7.3 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically adjust pH to 7.3. Pour into sterile Petri dishes. Use: For the isolation and cultivation of Salmonella species from foods. Trypticase™ Agar Base Composition per liter: Pancreatic digest of casein 20.0g Agar 3.5g Phenol Red 0.02g pH 7.4 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems and Oxoid Unipath. © 2010 by Taylor and Francis Group, LLC Trypticase™ Peptone Glucose Yeast Extract Broth, Buffered 1823 Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the differentiation of microorganisms based on their motility. Trypticase™ Agar Base with Carbohydrate Composition per liter: Pancreatic digest of casein 20.0g Carbohydrate 5.0g Agar 3.5g Phenol Red 0.02g pH 7.4 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes. Autoclave for 15 min at 13 psi pres- sure–118°C. Do not overheat. Pour into sterile Petri dishes or leave in tubes. Use: For differentiation of microorganisms based on their motility and fermentation reactions. Fermentation of carbohydrate turns the medium yellow. Trypticase™ Azolectin Tween™ Broth Base See: TAT Broth Base Trypticase™ Broth, Supplemented Composition per liter: Pancreatic digest of casein 20.0g MgSO 4 ·7H 2 O 0.015g FeCl 3 7.0mg pH 7.2 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Use: For the cultivation of Bacillus stearothermophilus. Trypticase™ Glucose Agar Composition per liter: Pancreatic digest of casein 17.0g Agar 15.0g NaCl 5.0g Papaic digest of soybean meal 3.0g Glucose 7.5g Yeast extract 3.0g KH 2 PO 4 2.5g Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation and maintenance of Lactococcus raffinolactis, Streptococcus equi, Streptococcus pneumoniae, and Streptococcus uberis. Trypticase™ Glucose Extract Agar Composition per liter: Agar 15.0g Pancreatic digest of casein 5.0g Beef extract 3.0g Glucose 1.0g pH 7.0 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems . Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the enumeration of bacteria in water, milk, and other speci- mens. Trypticase™ Novobiocin Broth (TN Broth) Composition per liter: Pancreatic digest of casein 17.0g NaCl 5.0g Papaic digest of soybean meal 3.0g K 2 HPO 4 2.5g Glucose 2.5g Bile salts No. 3 1.5g K 2 HPO 4 1.5g Novobiocin solution 10.0mL pH 7.3 ± 0.2 at 25°C Novobiocin Solution: Composition per 10.0mL: Novobiocin 0.02g Preparation of Novobiocin Solution: Add novobiocin to dis- tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, except novobiocin solu- tion, to distilled/deionized water and bring volume to 990.0mL. Mix thor- oughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile novobiocin solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the cultivation of verotoxin-producing Escherichia coli. Trypticase™ Peptone Glucose Yeast Extract Broth (TPGY Broth) Composition per liter: Pancreatic digest of casein 50.0g Yeast extract 20.0g Peptone 5.0g Glucose 4.0g Sodium thioglycolate 1.0g pH 7.0 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes in 15.0mL volumes. Autoclave for 10 min at 15 psi pressure–121°C. Use: For the cultivation of Clostridium botulinum. Trypticase™ Peptone Glucose Yeast Extract Broth, Buffered Composition per liter: Pancreatic digest of casein 50.0g Yeast extract 20.0g © 2010 by Taylor and Francis Group, LLC 1824 Trypticase™ Peptone Glucose Yeast Extract Broth with Trypsin Na 2 HPO 4 5.0g Peptone 5.0g Glucose 4.0g Sodium thioglycolate 1.0g pH 7.3 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat until dis- solved. Adjust pH to 7.3. Distribute into tubes in 15.0mL volumes. Au- toclave for 8 min at 15 psi pressure–121°C. Use: For the isolation and cultivation of Clostridium perfringens from foods. Trypticase™ Peptone Glucose Yeast Extract Broth with Trypsin (TPGYT Broth) Composition per 1067.0mL: Pancreatic digest of casein 50.0g Yeast extract 20.0g Peptone 5.0g Glucose 4.0g Sodium thioglycolate 1.0g Trypsin solution 67.0mL pH 7.0 ± 0.2 at 25°C Trypsin Solution: Composition per 100.0mL: Trypsin 1.5g Preparation of Trypsin Solution: Add trypsin to distilled/deion- ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster- ilize. Preparation of Medium: Add components, except trypsin solu- tion, to distilled/deionized water and bring volume to 1.0L. Mix thor- oughly. Gently heat and bring to boiling. Distribute into tubes in 15.0mL volumes. Autoclave for 10 min at 15 psi pressure–121°C. Im- mediately prior to use, aseptically add 1.0mL of sterile trypsin solution to each tube. Mix thoroughly. Use: For the cultivation of Clostridium botulinum. Trypticase™ Phytone™ Glucose Medium Composition per liter: Glucose 15.0g Pancreatic digest of casein 10.0g Agar 8.0g Papaic digest of soybean meal 5.0g Yeast extract 2.5g K 2 HPO 4 2.0g L-Cysteine·HCl·H 2 O 0.5g MgCl 2 0.5g ZnSO 4 ·7H 2 O 0.25g FeCl 3 1.0mg Preparation of Medium: Add ZnSO 4 to approximately 100.0mL of distilled/deionized water and dissolve. Add remaining components and bring volume to 1.0L with distilled/deionized water. Mix thor- oughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation and maintenance of Bifidobacterium species. Trypticase™ Phytone™ Glucose Medium with Tween™ 80 Composition per liter: Glucose 15.0g Pancreatic digest of casein 10.0g Agar 8.0g Papaic digest of soybean meal 5.0g Yeast extract 2.5g K 2 HPO 4 2.0g L-Cysteine·HCl·H 2 O 0.5g MgCl 2 0.5g ZnSO 4 ·7H 2 O 0.25g FeCl 3 1.0mg Tween™ 80 2.0mL Preparation of Medium: Add ZnSO 4 to approximately 100.0mL of distilled/deionized water and dissolve. Add remaining components and bring volume to 1.0L with distilled/deionized water. Mix thor- oughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation and maintenance of Bifidobacterium species. Trypticase™ Phytone Medium (DSMZ Medium 75) Composition per liter: Trypticase™ peptone 17.0g Phytone™ peptone 3.0g NaCl 5.0g K 2 HPO 4 2.5g Glucose 2.5g Distilled water 1000.0mL pH 7.3 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.3. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C. Use: For the cultivation and maintenance of Acinetobacter spp. Trypticase™ Phytone™ Medium Composition per liter: Pancreatic digest of casein 17.0g NaCl 5.0g Phytone™ peptone 3.0g Glucose 2.5g K 2 HPO 4 2.5g pH 7.3 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Use: For the cultivation of Acinetobacter species. Trypticase™ Serum Seawater Agar (ATCC Medium 1359) Composition per liter: Agar 12.0g Pancreatic digest of casein 1.0g Seawater 990.0mL Horse serum 10.0mL pH 7.3 ± 0.2 at 25°C © 2010 by Taylor and Francis Group, LLC . a 1.0-inch butt. Use: For the differentiation of members of the Enterobacteriaceae based on their fermentation of lactose, sucrose, and glucose and the production of H 2 S. Triple Sugar Iron Agar (TSI. a 1.0-inch butt. Use: For the differentiation of members of the Enterobacteriaceae based on their fermentation of lactose, sucrose, and glucose and the production of H 2 S. Triple Sugar Iron Agar (TSI. 1.0-inch butt. Use: For the differentiation of members of the Enterobacteriaceae based on their fermentation of lactose, sucrose, and glucose and the production of H 2 S. Triple Sugar Iron Agar, HiVeg Composition