Phenol Red Broth Base with Plant Extract No. 1 1385 Adonitol 5.0g Beef extract 1.0g Phenol Red 0.018g pH 7.4 ± 0.2 at 25°C Source: This medium is available from HiMedia. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes with inverted Durham tubes. Autoclave for 15 min at 15 psi pressure– 121°C. Use: For the detection of adonitol fermenting bacteria. Phenol Red Agar Composition per liter: Agar 15.0g Pancreatic digest of casein 10.0g NaCl 5.0g Phenol Red 0.018g Carbohydrate solution 20.0mL pH 7.4 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems. Carbohydrate Solution: Composition per 20.0mL: Carbohydrate 5.0–10.0g Preparation of Carbohydrate Solution: Add carbohydrate to distilled/deionized water and bring volume to 20.0mL. Mix thorough- ly. Filter sterilize. Preparation of Medium: Add components, except carbohydrate solution, to distilled/deionized water and bring volume to 980.0mL. Mix thoroughly. Adjust pH to 7.4 if necessary. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 20.0mL of sterile carbohydrate solution. Pour into sterile Petri dishes or distribute into sterile tubes. Allow tubes to cool in a slanted position. Use: For the determination of fermentation reactions. Bacteria that can ferment the added carbohydrate turn the medium yellow. Phenol Red Agar Composition per liter: Agar 15.0g Proteose peptone No. 3 10.0g NaCl 5.0g Beef extract 1.0g Phenol Red 0.025g Carbohydrate solution 20.0mL pH 7.4 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems. Carbohydrate Solution: Composition per 20.0mL: Carbohydrate 5.0–10.0g Preparation of Carbohydrate Solution: Add carbohydrate to distilled/deionized water and bring volume to 20.0mL. Mix thorough- ly. Filter sterilize. Preparation of Medium: Add components, except carbohydrate solution, to distilled/deionized water and bring volume to 980.0mL. Mix thoroughly. Adjust pH to 7.4 if necessary. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 20.0mL of sterile carbohydrate solution. Pour into sterile Petri dishes or distribute into sterile tubes. Allow tubes to cool in a slanted position. Use: For the determination of fermentation reactions. Bacteria that can ferment the added carbohydrate turn the medium yellow. Phenol Red Arabinose Broth Composition per liter: Proteose peptone 10.0g NaCl 5.0g Arabinose 5.0g Beef extract 1.0g Phenol Red 0.018g pH 7.4 ± 0.2 at 25°C Source: This medium is available from HiMedia. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes with inverted Durham tubes. Autoclave for 15 min at 15 psi pressure– 121°C. Use: For the detection of arabinose-fermenting bacteria. Phenol Red Broth Composition per liter: Pancreatic digest of casein 10.0g NaCl 5.0g Phenol Red 0.018g Carbohydrate solution 20.0mL pH 7.4 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems. Carbohydrate Solution: Composition per 20.0mL: Carbohydrate 5.0–10.0g Preparation of Carbohydrate Solution: Add carbohydrate to distilled/deionized water and bring volume to 20.0mL. Mix thorough- ly. Filter sterilize. Preparation of Medium: Add components, except carbohydrate so- lution, to distilled/deionized water and bring volume to 980.0mL. Mix thoroughly. Adjust pH to 7.4 if necessary. Distribute into tubes contain- ing an inverted Durham tube. Fill each tube with 9.8mL of medium. Au- toclave for 15 min at 13 psi pressure–118°C. Cool to 45°–50°C. Aseptically add 0.2mL of sterile carbohydrate solution to each tube. Use: For the determination of fermentation reactions in the differenti- ation of microorganisms. Fermentation is determined by the produc- tion of acid—broth turns yellow—and formation of gas—bubble trapped in Durham tube. Phenol Red Broth Base with Plant Extract No. 1 Composition per liter: Plant hydrolysate 10.0g NaCl 5.0g Plant extract No. 1 1.0g Phenol Red 0.018g pH 7.4 ± 0.2 at 25°C Source: This medium, without carbohydrate, is available as a pre- mixed powder from HiMedia. © 2010 by Taylor and Francis Group, LLC 1386 Phenol Red Carbohydrate Broth Carbohydrate Solution: Composition per 20.0mL: Carbohydrate 5.0–10.0g Preparation of Carbohydrate Solution: Add carbohydrate to distilled/deionized water and bring volume to 20.0mL. Mix thorough- ly. Filter sterilize. Preparation of Medium: Add components, except carbohydrate solution, to distilled/deionized water and bring volume to 980.0mL. Mix thoroughly. Adjust pH to 7.4 if necessary. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 20.0mL of sterile carbohydrate solution. Aseptically distribute into sterile tubes or flasks. Use: For the determination of fermentation reactions. Bacteria that can ferment the added carbohydrate turn the medium yellow. Phenol Red Carbohydrate Broth (BAM M121) Composition per liter: Pancreatic digest of casein 10.0g NaCl 5.0g Beef extract 1.0g Carbohydrate solution 200.0mL Phenol Red solution 7.2mL pH 7.4 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostics. Phenol Red Solution: Composition per 10.0mL: Phenol Red 0.025g Preparation of Phenol Red Solution: Add Phenol Red to dis- tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Carbohydrate Solution: Composition per 200.0mL: Carbohydrate 5.0–10.0g Preparation of Carbohydrate Solution: Add carbohydrate (5.0g dulcitol, 10.0g lactose, or 10.0g sucrose) to distilled/deionized water and bring volume to 200.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, except carbohydrate so- lution, to distilled/deionized water and bring volume to 800.0mL. Mix thoroughly. Adjust pH to 7.4 if necessary. Distribute into tubes contain- ing an inverted Durham tube. Fill each tube with 8.0mL of medium. Au- toclave for 15 min at 13 psi pressure–118°C. Cool to 45°–50°C. Aseptically add 2.0mL of sterile carbohydrate solution to each tube. Use: For the determination of fermentation reactions in the differenti- ation of microorganisms, such as Salmonella spp. Fermentation is de- termined by the production of acid—broth turns yellow—and formation of gas—bubble trapped in Durham tube. Phenol Red Carbohydrate Broth with Sodium Chloride (BAM M121) Composition per liter: NaCl 30.0g Pancreatic digest of casein 10.0g Beef extract 1.0g Carbohydrate solution 200.0mL Phenol Red solution 7.2mL pH 7.4 ± 0.2 at 25°C Source: This medium, without added NaCl, is available as a premixed powder from BD Diagnostics. Phenol Red Solution: Composition per 10.0mL: Phenol Red 0.025g Preparation of Phenol Red Solution: Add Phenol Red to dis- tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Carbohydrate Solution: Composition per 200.0mL: Carbohydrate 5.0–10.0g Preparation of Carbohydrate Solution: Add carbohydrate (5.0g dulcitol, 10.0g lactose, or 10.0g sucrose) to distilled/deionized water and bring volume to 200.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, except carbohydrate so- lution, to distilled/deionized water and bring volume to 800.0mL. Mix thoroughly. Adjust pH to 7.4 if necessary. Distribute into tubes contain- ing an inverted Durham tube. Fill each tube with 8.0mL of medium. Au- toclave for 15 min at 13 psi pressure–118°C. Cool to 45°–50°C. Aseptically add 2.0mL of sterile carbohydrate solution to each tube. Use: For the determination of fermentation reactions in the differenti- ation of halophilic Vibrio spp. Fermentation is determined by the pro- duction of acid—broth turns yellow—and formation of gas—bubble trapped in Durham tube. Phenol Red Dextrose Broth Composition per liter: Proteose peptone 10.0g NaCl 5.0g Glucose 5.0g Beef extract 1.0g Phenol Red 0.018g pH 7.4 ± 0.2 at 25°C Source: This medium is available from HiMedia. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes with inverted Durham tubes. Autoclave for 15 min at 15 psi pressure– 121°C. Use: For glucose fermentation studies of microorganisms. Phenol Red Dulcitol HiVeg Broth Composition per liter: Plant peptone No. 3 10.0g Dulcitol 5.0g NaCl 5.0g Plant extract 1.0g Phenol Red 0.018g pH 7.4 ± 0.2 at 25°C Source: This medium is available as a premixed powder from Hi- Media. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. © 2010 by Taylor and Francis Group, LLC Phenol Red Glucose HiVeg Agar 1387 Use: For the determination of the ability of a microorganism to ferment dulcitol. Fermentation is determined by the production of acid—medium turns yellow. Phenol Red Galactose Broth Composition per liter: Proteose peptone 10.0g NaCl 5.0g Galactose 5.0g Beef extract 1.0g Phenol Red 0.018g pH 7.4 ± 0.2 at 25°C Source: This medium is available from HiMedia. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes with inverted Durham tubes. Autoclave for 15 min at 15 psi pressure– 121°C. Use: For galactose fermentation studies of microorganisms. Phenol Red Glucose Broth Composition per liter: Pancreatic digest of casein 10.0g Glucose 5.0g NaCl 5.0g Phenol Red 0.018g pH 7.3 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.3 if necessary. Distribute into tubes containing an inverted Durham tube. Fill each tube with 10.0mL of medium. Autoclave for 15 min at 13 psi pressure–118°C. Use: For determination of the ability of a microorganism to ferment glucose. Fermentation is determined by the production of acid—broth turns yellow—and formation of gas—bubble trapped in Durham tube. Phenol Red Glucose Broth (BAM M122) Composition per liter: Proteose peptone No. 3 10.0g Glucose 5.0g NaCl 5.0g Beef extract 1.0g Phenol Red solution 7.2mL pH 7.4 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostics. Phenol Red Solution: Composition per 10.0mL: Phenol Red 0.025g Preparation of Phenol Red Solution: Add Phenol Red to dis- tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.4 if necessary. Distribute into tubes containing an inverted Durham tube. Fill each tube with 10.0mL of medium. Autoclave for 10 min at 13 psi pressure–118°C. Use: For determination of the ability of a microorganism to ferment glucose. Fermentation is determined by the production of acid—broth turns yellow—and formation of gas—bubble trapped in Durham tube. Phenol Red Glucose Broth, HiVeg Composition per liter: Plant peptone No. 3 10.0g Glucose 5.0g NaCl 5.0g Plant extract 1.0g Phenol Red 0.018g pH 7.3 ± 0.2 at 25°C Source: This medium is available as a premixed powder from Hi- Media. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.3 if necessary. Distribute into tubes containing an inverted Durham tube. Fill each tube with 10.0mL of medium. Autoclave for 15 min at 13 psi pressure–118°C. Use: For determination of the ability of a microorganism to ferment glucose. Fermentation is determined by the production of acid—broth turns yellow—and formation of gas—bubble trapped in Durham tube. Phenol Red Glucose Broth with Sodium Chloride (BAM M122) Composition per liter: NaCl 30.0g Proteose peptone No. 3 10.0g Glucose 5.0g Beef extract 1.0g Phenol Red solution 7.2mL pH 7.4 ± 0.2 at 25°C Source: This medium, without added NaCl, is available as a premixed powder from BD Diagnostics. Phenol Red Solution: Composition per 10.0mL: Phenol Red 0.025g Preparation of Phenol Red Solution: Add Phenol Red to dis- tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.4 if necessary. Distribute into tubes containing an inverted Durham tube. Fill each tube with 10.0mL of medium. Autoclave for 10 min at 13 psi pressure–118°C. Use: For determination of the ability of halophilic Vibrio spp. to fer- ment glucose. Fermentation is determined by the production of acid— broth turns yellow—and formation of gas—bubble trapped in Durham tube. Phenol Red Glucose HiVeg Agar Composition per liter: Agar 15.0g Plant peptone No. 3 10.0g Glucose 10.0g NaCl 5.0g © 2010 by Taylor and Francis Group, LLC 1388 Phenol Red HiVeg Agar Base with Carbohydrate Plant extract 1.0g Phenol Red 0.025g pH 7.4 ± 0.2 at 25°C Source: This medium is available as a premixed powder from Hi- Media. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 13 psi pressure–118°C. Pour into sterile Petri dishes or leave in tubes. Al- low tubes to cool in a slanted position. Use: For the determination of the ability of a microorganism to ferment glucose. Fermentation is determined by the production of acid—medium turns yellow. Phenol Red HiVeg Agar Base with Carbohydrate Composition per liter: Agar 15.0g Plant peptone No. 3 10.0g NaCl 5.0g Plant extract 1.0g Phenol Red 0.025g Carbohydrate solution 20.0mL pH 7.4 ± 0.2 at 25°C Source: This medium, without carbohydrate, is available as a pre- mixed powder from HiMedia. Carbohydrate Solution: Composition per 20.0mL: Carbohydrate 5.0–10.0g Preparation of Carbohydrate Solution: Add carbohydrate to distilled/deionized water and bring volume to 20.0mL. Mix thorough- ly. Filter sterilize. Preparation of Medium: Add components, except carbohydrate solution, to distilled/deionized water and bring volume to 980.0mL. Mix thoroughly. Adjust pH to 7.4 if necessary. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 20.0mL of sterile carbohydrate solution. Pour into sterile Petri dishes or distribute into sterile tubes. Allow tubes to cool in a slanted position. Use: For the determination of fermentation reactions. Bacteria that can ferment the added carbohydrate turn the medium yellow. Phenol Red HiVeg Broth Base Composition per liter: Plant peptone No. 3 10.0g NaCl 5.0g Plant extract 1.0g Phenol Red 0.018g Carbohydrate solution 20.0mL pH 7.4 ± 0.2 at 25°C Source: This medium, without carbohydrate, is available as a pre- mixed powder from HiMedia. Carbohydrate Solution: Composition per 20.0mL: Carbohydrate 5.0–10.0g Preparation of Carbohydrate Solution: Add carbohydrate to distilled/deionized water and bring volume to 20.0mL. Mix thorough- ly. Filter sterilize. Preparation of Medium: Add components, except carbohydrate solution, to distilled/deionized water and bring volume to 980.0mL. Mix thoroughly. Adjust pH to 7.4 if necessary. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 20.0mL of sterile carbohydrate solution. Aseptically distribute into sterile tubes or flasks. Use: For the determination of fermentation reactions. Bacteria that can ferment the added carbohydrate turn the medium yellow. Phenol Red Inositol Broth Composition per liter: Proteose peptone 10.0g NaCl 5.0g Inositol 5.0g Beef extract 1.0g Phenol Red 0.018g pH 7.4 ± 0.2 at 25°C Source: This medium is available from HiMedia. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes with inverted Durham tubes. Autoclave for 15 min at 15 psi pressure– 121°C. Use: For inositol fermentation studies of microorganisms. Phenol Red Lactose Agar Composition per liter: Agar 15.0g Lactose 10.0g Proteose peptone No. 3 10.0g NaCl 5.0g Beef extract 1.0g Phenol Red 25.0mg pH 7.4 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 13 psi pressure–118°C. Pour into sterile Petri dishes or leave in tubes. Al- low tubes to cool in a slanted position. Use: For the determination of the ability of a microorganism to ferment lactose. Fermentation is determined by the production of acid—medium turns yellow. Phenol Red Lactose Broth Composition per liter: Pancreatic digest of casein 10.0g Lactose 5.0g NaCl 5.0g Phenol Red 0.018g pH 7.3 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.3 if necessary. Distribute into tubes containing an inverted Durham tube. © 2010 by Taylor and Francis Group, LLC Phenol Red Maltose HiVeg Broth 1389 Fill each tube with 10.0mL of medium. Autoclave for 15 min at 13 psi pressure–118°C. Use: For determination of the ability of a microorganism to ferment lactose. Fermentation is determined by the production of acid—broth turns yellow—and formation of gas—bubble trapped in Durham tube. Phenol Red Lactose Broth Composition per liter: Proteose peptone 10.0g NaCl 5.0g Lactose 5.0g Beef extract 1.0g Phenol Red 0.018g pH 7.4 ± 0.2 at 25°C Source: This medium is available from HiMedia. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes with inverted Durham tubes. Autoclave for 15 min at 15 psi pressure– 121°C. Use: For the detection of lactose-fermenting bacteria. Phenol Red Lactose Broth Composition per liter: Peptic digest of animal tissue 10.0g Lactose 10.0g NaCl 5.0g Beef extract 1.0g Phenol Red 0.018g pH 7.4 ± 0.2 at 25°C Source: This medium is available from HiMedia. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes with inverted Durham tubes. Autoclave for 15 min at 15 psi pressure– 121°C. Use: For lactose fermentation studies in accordance with the ISO Committee under the specification ISO 9308-1:1990. Phenol Red Lactose HiVeg Agar Composition per liter: Agar 15.0g Plant peptone No. 3 10.0g Lactose 10.0g NaCl 5.0g Plant extract 1.0g Phenol Red 0.025g pH 7.4 ± 0.2 at 25°C Source: This medium is available as a premixed powder from Hi- Media. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 13 psi pressure–118°C. Pour into sterile Petri dishes or leave in tubes. Al- low tubes to cool in a slanted position. Use: For the determination of the ability of a microorganism to ferment lactose. Fermentation is determined by the production of acid—medium turns yellow. Phenol Red Lactose HiVeg Broth Composition per liter: Plant peptone No. 3 10.0g Lactose 5.0g NaCl 5.0g Plant extract 1.0g Phenol Red 0.018g pH 7.4 ± 0.2 at 25°C Source: This medium is available as a premixed powder from Hi- Media. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 13 psi pressure–118°C. Use: For the determination of the ability of a microorganism to ferment lactose. Fermentation is determined by the production of acid—medium turns yellow. Phenol Red Maltose Broth Composition per liter: Proteose peptone 10.0g NaCl 5.0g Maltose 5.0g Beef extract 1.0g Phenol Red 0.018g pH 7.4 ± 0.2 at 25°C Source: This medium is available from HiMedia. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes with inverted Durham tubes. Autoclave for 15 min at 15 psi pressure– 121°C. Use: For maltose fermentation studies of microorganisms. Phenol Red Maltose HiVeg Agar Composition per liter: Agar 15.0g Plant peptone No. 3 10.0g Maltose 10.0g NaCl 5.0g Plant extract 1.0g Phenol Red 0.025g pH 7.4 ± 0.2 at 25°C Source: This medium is available as a premixed powder from Hi- Media. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 13 psi pressure–118°C. Pour into sterile Petri dishes or leave in tubes. Al- low tubes to cool in a slanted position. Use: For the determination of the ability of a microorganism to ferment maltose. Fermentation is determined by the production of acid—medium turns yellow. Phenol Red Maltose HiVeg Broth Composition per liter: Plant peptone No. 3 10.0g Maltose 5.0g © 2010 by Taylor and Francis Group, LLC 1390 Phenol Red Mannitol Agar NaCl 5.0g Plant extract 1.0g Phenol Red 0.018g pH 7.4 ± 0.2 at 25°C Source: This medium is available as a premixed powder from Hi- Media. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 13 psi pressure–118°C. Use: For the determination of the ability of a microorganism to ferment maltose. Fermentation is determined by the production of acid—medium turns yellow. Phenol Red Mannitol Agar Composition per liter: Agar 15.0g Mannitol 10.0g Proteose peptone No. 3 10.0g NaCl 5.0g Beef extract 1.0g Phenol Red 25.0mg pH 7.4 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 13 psi pressure–118°C. Pour into sterile Petri dishes or leave in tubes. Allow tubes to cool in a slanted position. Use: For determination of the ability of a microorganism to ferment mannitol. Fermentation is determined by the production of acid— medium turns yellow. Phenol Red Mannitol Broth Composition per liter: Pancreatic digest of casein 10.0g D-Mannitol 5.0g NaCl 5.0g Phenol Red 0.018g pH 7.3 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.3 if necessary. Distribute into tubes containing an inverted Durham tube. Fill each tube with 10.0mL of medium. Autoclave for 15 min at 13 psi pressure–118°C. Use: For determination of the ability of a microorganism to ferment mannitol. Fermentation is determined by the production of acid—broth turns yellow—and formation of gas—bubble trapped in Durham tube. Phenol Red Mannitol HiVeg Agar Composition per liter: Agar 15.0g Plant peptone No. 3 10.0g Mannitol 10.0g NaCl 5.0g Plant extract 1.0g Phenol Red 0.025g pH 7.4 ± 0.2 at 25°C Source: This medium is available as a premixed powder from Hi- Media. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 13 psi pressure–118°C. Pour into sterile Petri dishes or leave in tubes. Al- low tubes to cool in a slanted position. Use: For the determination of the ability of a microorganism to ferment mannitol. Fermentation is determined by the production of acid—medium turns yellow. Phenol Red Mannitol HiVeg Broth Composition per liter: Plant peptone No. 3 10.0g Mannitol 5.0g NaCl 5.0g Plant extract 1.0g Phenol Red 0.018g pH 7.4 ± 0.2 at 25°C Source: This medium is available as a premixed powder from Hi- Media. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 13 psi pressure–118°C. Use: For the determination of the ability of a microorganism to ferment mannitol. Fermentation is determined by the production of acid—medium turns yellow. Phenol Red Raffinose Broth Composition per liter: Proteose peptone 10.0g NaCl 5.0g Raffinose 5.0g Beef extract 1.0g Phenol Red 0.018g pH 7.4 ± 0.2 at 25°C Source: This medium is available from HiMedia. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes with inverted Durham tubes. Autoclave for 15 min at 15 psi pressure– 121°C. Use: For raffinose fermentation studies of microorganisms. Phenol Red Rhamnose Broth Composition per liter: Proteose peptone 10.0g NaCl 5.0g Rhamnose 5.0g Beef extract 1.0g Phenol Red 0.018g pH 7.4 ± 0.2 at 25°C Source: This medium is available from HiMedia. © 2010 by Taylor and Francis Group, LLC Phenol Red Sucrose HiVeg Broth 1391 Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes with inverted Durham tubes. Autoclave for 15 min at 15 psi pressure– 121°C. Use: For rhamnose fermentation studies of microorganisms. Phenol Red Salicin Broth Composition per liter: Proteose peptone 10.0g NaCl 5.0g Salicin 5.0g Beef extract 1.0g Phenol Red 0.018g pH 7.4 ± 0.2 at 25°C Source: This medium is available from HiMedia. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes with inverted Durham tubes. Autoclave for 15 min at 15 psi pressure– 121°C. Use: For salicin fermentation studies of microorganisms. Phenol Red Sorbitol Broth Composition per liter: Proteose peptone 10.0g NaCl 5.0g Sorbitol 5.0g Beef extract 1.0g Phenol Red 0.018g pH 7.4 ± 0.2 at 25°C Source: This medium is available from HiMedia. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes with inverted Durham tubes. Autoclave for 15 min at 15 psi pressure– 121°C. Use: For sorbitol fermentation studies of microorganisms. Phenol Red Starch Broth Composition per liter: Proteose peptone 10.0g NaCl 5.0g Starch 5.0g Beef extract 1.0g Phenol Red 0.018g pH 7.4 ± 0.2 at 25°C Source: This medium is available from HiMedia. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes with inverted Durham tubes. Autoclave for 15 min at 15 psi pressure– 121°C. Use: For starch fermentation studies of microorganisms. Phenol Red Sucrose Broth Composition per liter: Pancreatic digest of casein 10.0g NaCl 5.0g Sucrose 5.0g Phenol Red 0.018g pH 7.3 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.3 if necessary. Distribute into tubes containing an inverted Durham tube. Fill each tube with 10.0mL of medium. Autoclave for 15 min at 13 psi pressure–118°C. Use: For determination of the ability of a microorganism to ferment sucrose. Fermentation is determined by the production of acid—broth turns yellow—and formation of gas—bubble trapped in Durham tube. Phenol Red Sucrose Broth Composition per liter: Proteose peptone 10.0g NaCl 5.0g Sucrose 5.0g Beef extract 1.0g Phenol Red 0.018g pH 7.4 ± 0.2 at 25°C Source: This medium is available from HiMedia. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes with inverted Durham tubes. Autoclave for 15 min at 15 psi pressure– 121°C. Use: For sucrose fermentation studies of microorganisms. Phenol Red Sucrose HiVeg Agar Composition per liter: Agar 15.0g Plant peptone No. 3 10.0g Sucrose 10.0g NaCl 5.0g Plant extract 1.0g Phenol Red 0.025g pH 7.4 ± 0.2 at 25°C Source: This medium is available as a premixed powder from Hi- Media. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 13 psi pressure–118°C. Pour into sterile Petri dishes or leave in tubes. Al- low tubes to cool in a slanted position. Use: For the determination of the ability of a microorganism to ferment sucrose. Fermentation is determined by the production of acid—medium turns yellow. Phenol Red Sucrose HiVeg Broth Composition per liter: Plant peptone No. 3 10.0g NaCl 5.0g Sucrose 5.0g Plant extract 1.0g Phenol Red 0.018g pH 7.4 ± 0.2 at 25°C © 2010 by Taylor and Francis Group, LLC 1392 Phenol Red Tartrate Agar Source: This medium is available as a premixed powder from Hi- Media. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 13 psi pressure–118°C. Use: For the determination of the ability of a microorganism to ferment sucrose. Fermentation is determined by the production of acid—medium turns yellow. Phenol Red Tartrate Agar Composition per liter: Agar 15.0g Peptone 10.0g Potassium tartrate 10.0g NaCl 5.0g Phenol Red 0.024g pH 7.6 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems. Preparation of Medium: Add components to cold distilled/deion- ized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 13 psi pressure–118°C. Pour into sterile Petri dishes or leave in tubes. Allow tubes to cool in an upright position. Use: For the differentiation of Gram-negative bacteria of the intestinal groups, particularly members of the Salmonella (paratyphoid) group based on their ability to ferment tartrate. Phenol Red Tartrate Broth Composition per liter: Pancreatic digest of casein 10.0g Potassium tartrate 10.0g Agar 5.0g NaCl 5.0g Phenol Red 0.024g pH 7.6 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Use: For the differentiation of Gram-negative bacteria of the intestinal groups, particularly members of the Salmonella (paratyphoid) group based on their ability to ferment tartrate. Phenol Red Tartrate HiVeg Agar Composition per liter: Agar 15.0g Plant peptone 10.0g Sodium potassium tartrate 10.0g NaCl 5.0g Phenol Red 0.024g pH 7.6 ± 0.2 at 25°C Source: This medium is available as a premixed powder from Hi- Media. Preparation of Medium: Add components to cold distilled/deion- ized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 13 psi pressure–118°C. Pour into sterile Petri dishes or leave in tubes. Allow tubes to cool in an upright position. Use: For the differentiation of Gram-negative bacteria of the intestinal groups, particularly members of the Salmonella (paratyphoid) group based on their ability to ferment tartrate. Phenol Red Trehalose Broth Composition per liter: Proteose peptone 10.0g NaCl 5.0g Trehalose 5.0g Beef extract 1.0g Phenol Red 0.018g pH 7.4 ± 0.2 at 25°C Source: This medium is available from HiMedia. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes with inverted Durham tubes. Autoclave for 15 min at 15 psi pressure– 121°C. Use: For trehalose fermentation studies of microorganisms. Phenol Red Xylose Broth Composition per liter: Proteose peptone 10.0g NaCl 5.0g Xylose 5.0g Beef extract 1.0g Phenol Red 0.018g pH 7.4 ± 0.2 at 25°C Source: This medium is available from HiMedia. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes with inverted Durham tubes. Autoclave for 15 min at 15 psi pressure– 121°C. Use: For xylose fermentation studies of microorganisms. Phenolphthalein Phosphate HiVeg Agar Composition per liter: Agar 15.0g Plant peptone 5.0g NaCl 5.0g Plant extract 3.0g Sodium phenolphthalein phosphate 0.012g pH 7.4 ± 0.2 at 25°C Source: This medium is available as a premixed powder from Hi- Media. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–21°C. Use: For the identification of phosphatase-positive Staphylococcus au- reus. Phenylalanine Agar (Phenylalanine Deaminase Medium) Composition per liter: Agar 12.0g NaCl 5.0g Yeast extract 3.0g © 2010 by Taylor and Francis Group, LLC Phenylobacterium Agar 1393 DL-Phenylalanine 2.0g Na 2 HPO 4 1.0g pH 7.3 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling. Distribute into tubes or flasks. Autoclave for 10 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the differentiation of enteric Gram-negative bacilli on the basis of their ability to produce phenylpyruvic acid from phenylala- nine. After appropriate incubation of bacteria, ferric chloride reagent is added on the agar. Formation of a green color in 1–5 min indicates the production of phenylpyruvic acid. Phenylalanine Malonate Broth Composition per liter: Sodium malonate 3.0g DL-Phenylalanine 2.0g NaCl 2.0g (NH 4 ) 2 SO 4 2.0g Yeast extract 1.0g K 2 HPO 4 0.6g KH 2 PO 4 0.4g Bromthymol Blue 0.025g pH 7.3 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 10 min at 10 psi pressure–115°C. Use: For the differentiation of Gram-negative enteric bacilli on the basis of malonate utilization and formation of pyruvic acid from phe- nylalanine. Phenylethanol Agar Composition per liter: Agar 15.0g Tryptose 10.0g NaCl 5.0g Beef extract 3.0g Phenylethanol 2.5g pH 7.3 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the isolation of staphylococci and streptococci from speci- mens containing a mixed flora. Phenylethanol Blood Agar Composition per liter: Agar 15.0g Tryptose 10.0g NaCl 5.0g Beef extract 3.0g Phenylethanol 2.5g Blood, defibrinated 50.0mL pH 7.3 ± 0.2 at 25°C Preparation of Medium: Add components, except blood, to dis- tilled/deionized water and bring volume to 950.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 13 psi pres- sure–118°C. Cool to 45°–50°C. Aseptically add sterile defibrinated blood. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the isolation of staphylococci and streptococci from speci- mens containing a mixed flora. Phenylethyl Alcohol Agar See: Phenethyl Alcohol Agar Phenylethyl Alcohol HiVeg Agar Composition per liter: Agar 15.0g Plant hydrolysate 15.0g Papaic digest of soybean meal 5.0g NaCl 5.0g Phenylethyl alcohol 2.5g pH 7.3 ± 0.2 at 25°C Source: This medium is available as a premixed powder from HiMedia. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the isolation of staphylococci and streptococci from speci- mens containing a mixed flora. Phenylketonuria Test Agar See: PKU Test Agar Phenylobacterium Agar Composition per liter: Agar 20.0g Chloridazon herbicide 2.0g Peptone 1.0g Yeast extract 1.0g Na 2 HPO 4 ·12H 2 O 0.7g (NH 4 ) 2 HPO 4 0.7g KH 2 PO 4 0.3g NH 4 H 2 PO 4 0.3g MgSO 4 ·7H 2 O 0.25g (NH 4 ) 2 SO 4 0.1g CaCl 2 ·6H 2 O 0.05g H 3 BO 3 0.5mg MnSO 4 ·4H 2 O 0.4mg ZnSO 4 ·7H 2 O 0.4mg FeCl 3 ·6H 2 O 0.2mg (NH 4 ) 6 Mo 7 O 24 ·4H 2 O 0.2mg Biotin 0.1mg KJ 0.1mg CuSO 4 ·5H 2 O 0.04mg Vitamin B 12 0.03mg pH 6.8–7.0 at 25°C © 2010 by Taylor and Francis Group, LLC 1394 Phenylobacterium Medium Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation and maintenance of Phenylobacterium immo- bile. Phenylobacterium Medium Composition per liter: Agar 15.0g Antipyrin 1.0g Na 2 HPO 4 ·12H 2 O 0.7g (NH 4 ) 2 HPO 4 0.7g KH 2 PO 4 0.3g NH 4 H 2 PO 4 0.3g MgSO 4 ·7H 2 O 0.25g FeCl 3 ·6H 2 O 0.2g (NH 4 ) 2 SO 4 0.1g CaCl 2 ·6H 2 O 50.0mg H 3 BO 3 0.5mg MnSO 4 ·4H 2 O 0.4mg ZnSO 4 ·7H 2 O 0.4mg (NH 4 ) 2 MoO 4 0.2mg Biotin 0.1mg CuSO 4 ·5H 2 O 0.1mg KI 0.1mg Vitamin B 12 30.0μg pH 6.8 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation of Phenylobacterium immobile. Phenylobacterium Medium Composition per liter: Antipyrine 1.0g Na 2 HPO 4 ·12H 2 O 0.7g (NH 4 ) 2 HPO 4 0.7g KH 2 PO 4 0.3g NH 4 H 2 PO 4 0.3g (NH 4 ) 2 SO 4 0.1g MgSO 4 ·7H 2 O 0.25g CaCl 2 ·6H 2 O 0.05g H 3 BO 3 0.5mg MnSO 4 ·4H 2 O 0.4mg ZnSO 4 ·7H 2 O 0.4mg FeCl 3 ·6H 2 O 0.2mg (NH 4 ) 2 MoO 4 0.2mg Biotin 0.1mg KI 0.1mg CuSO 4 ·5H 2 O 0.04mg Vitamin B 12 0.03mg Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Use: For the cultivation and maintenance of Phenylobacterium immobile. Phosphate Mineral Salts Medium with Octane Composition per liter: (NH 4 ) 2 HPO 4 10.0g K 2 HPO 4 5.0g Na 2 SO 4 0.5g Octane 10.0mL Preparation of Medium: Add components, except octane, to tap water and bring volume to 990.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Prior to inoculation, filter sterilize oc- tane. Aseptically add sterile octane to sterile medium. Aseptically dis- tribute into sterile tubes or flasks. Use: For the cultivation and maintenance of Pseudomonas oleo- vorans. Photobacterium Agar (DSMZ Medium 32) Composition per liter: Seawater aquarium salt 33.0g Agar 20.0g Tris 6.0g Yeast extract 5.0g Tryptone 5.0g NH 4 Cl 5.0g Glycerol 3.0g CaCO 3 1.0g pH 7.2–7.5 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2– 7.5. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation and maintenance of Vibrio fischeri. Photobacterium Agar Composition per liter: Seawater aquarium salt 33.0g Agar 20.0g Tris(hydroxymethyl)aminomethane 6.0g NH 4 Cl 5.0g Pancreatic digest of casein 5.0g Yeast extract 5.0g Glycerol 3.0g CaCO 3 1.0g pH 7.2 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation and maintenance of Acetobacter pasteur- ianus. Photobacterium Broth (DSMZ Medium 608) Composition per liter: NaCl 30.0g Na-glycerol phosphate 23.5g Tryptone 5.0g KH 2 PO 4 3.0g © 2010 by Taylor and Francis Group, LLC . 10.0mL of medium. Autoclave for 15 min at 13 psi pressure–118°C. Use: For determination of the ability of a microorganism to ferment glucose. Fermentation is determined by the production of acid—broth turns. 10.0mL of medium. Autoclave for 10 min at 13 psi pressure–118°C. Use: For determination of the ability of a microorganism to ferment glucose. Fermentation is determined by the production of acid—broth turns. 10.0mL of medium. Autoclave for 15 min at 13 psi pressure–118°C. Use: For determination of the ability of a microorganism to ferment glucose. Fermentation is determined by the production of acid—broth turns