Microarray Technology and Its Applications pptx

[...]... hundreds of thousands of chemical compounds can be tested to identify those compounds that elicit a desired effect Efficiency in the high throughput screening laboratory is obtained with miniaturization (96–, 384– and 1536–well microplates) and with extensive automation and plate handling robotics Besides performance criteria such as sensitivity and reproducibility, the success of a technology in this... culturing cells and screening compounds is the same regardless of whether a single or multiple measurements are made 1.3 Arrays for Nucleic Acid Analysis Several review articles covering advances and applications of DNA microarray technology have recently been published [17, 18] hence, the same material will not be repeated here Oligonucleotide and cDNA arrays have different strengths and weaknesses... presence and the titer of antibodies to particular antigens This approach lends itself to develop broad–spectrum tests for certain autoimmune diseases and for exposure to infectious agents As for nucleic acids, bead arrays also lend themselves to proteomic applications The technological challenges that remain are the development of specific, high affinity ligands that can be produced on a large scale and in... Anne Kallioniemi University of Tampere Laboratory of Cancer Genetics Institute of Medical Technology P.O Box 607 FIN-33014 University of Tampere, Finland anne.kallioniemi@uta.fi Olli Kallioniemi Medical Biotechnology Group VTT Technical Research Centre of Finland University of Turku P.O Box 106, 20521 Turku, Finland Olli.Kallioniemi@vtt.fi Ravi S Kane Rensselaer Polytechnic Institute Howard P Isermann... Martel, Matthew P Rounseville, and Ihab W Botros, and Bruce E Seligmann 1.1 Introduction Arrays have become an increasingly diverse set of tools for biological studies; their use continues to expand rapidly Likewise, the underlying array technologies, formats and protocols continue to evolve Investigators can choose from a growing range of options when selecting an array technology that is appropriate... 275 Part III Protein Microarrays 14 Protein, Antibody and Small Molecule Microarrays Hendrik Weiner, J¨rn Gl¨kler, Claus Hultschig, Konrad B¨ssow, o o u Gerald Walter 279 14.1 Introduction 279 14.2 Protein Microarrays 280 14.3 Antibody Microarrays ... aeing@nanofilm.de Ye Fang Corning, Inc Biochemical Sciences, Science and Technology Division Corning, NY 14870, USA fangy@corning.com Luisa Filipponi Swinburne University of Technology Industrial Research Institute Swinburne 533-545 Burwood Road Hawthorn, VIC 3122, Australia lfilipponi@swin.edu.au Anthony G Frutos Corning, Inc Biochemical Sciences, Science and Technology Division Corning, NY 14870, USA frutosag@corning.com... Kristi L Hanson Swinburne University of Technology Industrial Research Institute Swinburne 533-545 Burwood Road Hawthorn, VIC 3122, Australia khanson@swin.edu.au List of Contributors XIX Yulong Hong Corning, Inc Biochemical Sciences, Science and Technology Division Corning, NY 14870, USA hongy@corning.com Joydeep Lahiri Corning, Inc Biochemical Sciences, Science and Technology Division Corning, NY 14870,... modifications, such as phosphorylation and amidation, are also technical features that need to be addressed It is likely that different types of protein arrays will be required for cataloging the proteome, detecting differences in expression, and for screening compounds For a more extensive review on the development of protein-detecting microarrays and related devices see Kodadek [24] and Schweitzer [3] The development... sequence and was 3 -modified with heptylamine Arrays were printed with a PixSys 3000 microarrayer equipped with 85 µm inner diameter ceramic dispensing tips (Cartesian Technologies, Irvine, CA) in an environmental chamber (26◦ C and 80% relative humidity) Oligonucleotides and Antibodies The 16 target human mRNA species each required three oligonucleotides: A nuclease protection probe, a programming linker and . microarray technology: fundamentals, designed to provide a scientific base; fabrication, which describes the current state of the art and compares ‘old’ and new ways of building microarrays; and applications, . manner, and has since found equally exciting and useful applications in the study of proteins, metabolites, toxins, viruses, whole cells and even tissues. Although still relatively early in its evolution, microarray. Physics, New York University , New York, New Yo rk, USA U.R. M ¨ uller D.V. Nicolau (Eds.) Microarray Technology and Its Applications With 123 Figures Including 16 Color Plates 123 Uwe R. M ¨ uller, Ph.D. V.