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MINISTRY OF EDUCATION AND TRAINING HO CHI MINH CITY UNIVERSITY OF TECHNOLOGY AND EDUCATION FACULTY FOR HIGH QUALITY TRAINING GRADUATION THESIS FOOD TECHNOLOGY EFFECTS OF CAPPING AGENT CONCENTRATION AND REACTION TIME ON ANTIMICROBIAL ACTIVITIES OF COPPER NANOPARTICLES (CUNPs) SUPERVISOR: TRINH KHANH SON STUDENT: TRAN THI BAO CHAU DONG THAO DUYEN SKL009157 Ho Chi Minh City, August, 2022 HO CHI MINH CITY UNIVERSITY OF TECHNOLOGY AND EDUCATION FACULTY FOR HIGH QUALITY TRAINING GRADUATION PROJECT Thesis code 2022-18116005 EFFECTS OF CAPPING AGENT CONCENTRATION AND REACTION TIME ON ANTIMICROBIAL ACTIVITIES OF COPPER NANOPARTICLES (CUNPs) TRAN THI BAO CHAU Student ID: 18116005 DONG THAO DUYEN 18116007 Major: FOOD TECHNOLOGY Supervisor: TRINH KHANH SON, ASSOC PROF Ho Chi Minh City, August 2022 HO CHI MINH CITY UNIVERSITY OF TECHNOLOGY AND EDUCATION FACULTY FOR HIGH QUALITY TRAINING GRADUATION PROJECT Thesis code 2022-18116005 EFFECTS OF CAPPING AGENT CONCENTRATION AND REACTION TIME ON ANTIMICROBIAL ACTIVITIES OF COPPER NANOPARTICLES (CUNPs) TRAN THI BAO CHAU Student ID: 18116005 DONG THAO DUYEN 18116007 Major: FOOD TECHNOLOGY Supervisor: TRINH KHANH SON, ASSOC PROF Ho Chi Minh City, August 2022 DECLARATION As a result, we declare that all content presented in this graduation thesis has been carried out by us, including instructors and students The research content is based on the requirements, design, and guidelines and is validated by the instructor The entire content of the graduation thesis has been checked against plagiarism using Turnitin software, ensuring no more than 30% duplication We certify that regulations have correctly and fully cited the contents referenced in the graduation thesis _, August 2022 Signature i ACKNOWLEDGEMENT To complete this graduation thesis, first and foremost, we would like to thank the Faculty of Chemical and Food Technology, University of Technical Education, Ho Chi Minh City, for creating all conditions for equipment and facilities to help us complete the thesis Moreover, we would like to thank our adored supervisor, Trinh Khanh Son, Assoc Prof This paper could have never been accomplished without their assistance and dedicated involvement in every process step We are extremely grateful to them for supporting and understanding us during the past time We are also grateful to the 2017 and 2019 students who supported us in the research process With limited experience and conditions, implementing the thesis inevitably has shortcomings We look forward to receiving the attention and comments of teachers to improve our report Our team sincerely thanks ii iii iv v vi [75] [76] [77] [78] [79] [80] [81] [82] 8, 2021, doi: 10.3390/molecules26082290 E Chem, “Aggregation kinetics and surface charge of CuO.pdf” M Guzman, M Arcos, and J Dille, “Effect of the Concentration and the Type of Dispersant on the Synthesis of Copper Oxide Nanoparticles and Their Potential Antimicrobial Applications,” 2021, doi: 10.1021/acsomega.1c00818 W Wu, W Zhao, Y Wu, C Zhou, L Li, and Z Liu, “Applied Surface Science Antibacterial behaviors of Cu O particles with controllable morphologies in acrylic coatings,” Appl Surf Sci., vol 465, no August 2018, pp 279–287, 2019, doi: 10.1016/j.apsusc.2018.09.184 H Lee, J Y Song, and B S Kim, “Biological synthesis of copper nanoparticles using Magnolia kobus leaf extract and their antibacterial activity,” no February, 2013, doi: 10.1002/jctb.4052 O Article, L Benhalima, S Amri, M Bensouilah, and R Ouzrout, “Antibacterial effect of copper sulfate against multi-drug resistant nosocomial pathogens isolated from clinical samples,” vol 35, no 5, pp 1322–1328, 2019 B Bagchi et al., “In situ synthesis and antibacterial activity of copper nanoparticle loaded natural montmorillonite clay based on contact inhibition and ion release,” vol 108, pp 358–365, 2013 S Dashrath Bansod, “Synthesis and evaluation of antimicrobial potential of copper nanoparticle against agriculturally important Phytopathogens Production of Biofertilizer amd micronutrients View project,” no October, 2016, [Online] Available: https://www.researchgate.net/publication/309703772 Y Ohsumi, K Kitamoto, and Y Anraku, “Changes Induced in the Permeability Barrier of the Yeast Plasma Membrane by Cupric Ion,” vol 170, no 6, pp 2676–2682, 1988 49 Appendix The average crystal size Between Groups Within Groups Total The average crystal size Sample N Subset for alpha = 0.05 t20 S2,29 7.4990 t20 S1,15 7.9274 t20 S3,06 8.1248 Duncan t30 S0,76 t30 S2,67 8.6475 9.0671 t30 S1,91 9.3022 a Sig Means for groups in homogeneous subsets are displayed a Uses Harmonic Mean Sample Size = 5.000 .347 50 Appendix SEM images of t20 S1,15 51 Appendix SEM images of t20 S2,29 52 Appendix SEM images of t20 S3,07 53 Appendix SEM images of t30 S0,76 54 Appendix SEM images of t30 S1,91 55 Appendix SEM images of t30 S2,67 56 Appendix TEM images of t20 S2,29 57 Appendix TEM images of t30 S0,76 58 Appendix 10 Receipt of Delivery 59 60 Appendix 11 Check plagiarism by Turnitin software 61