I n tern ati o n al I N TE R N ATI ON AL O R G AN I ZATI O N S tarch es FOR an d ph o sph o ru s Am i d o n s, Fi rst U DC fkcu l e s ed i ti on 664 D e scri pto rs : et - S TAN D AR D I ZATI ON WE I KJ J YH AP O AH AR d e ri ved co n ten t pro d u i rs S tan d ard d &i v& - OP TAH M AWl R pro d u cts - D eterm i n ati on fl 46 C TAH ~ AP TM AWl M ~ R G AN I S ATI ON - I N TE R N ATI O N ALE D eterm i n ati o n S pectro ph o to m etri c de Ia ten eu r en P h osph o re total of DE N OR M ALI S ATI ON to tal m eth o d - M kth od e spectroph otom etri q u e 82- 2- 01 Ref : 543 847 starch e s, C h e m i cal an al ysi s, d ete rm i n ati on of ten t, ph osph oru s, spe ctroph o to m e tri c N o I SO 946-1 982 (E I an al ys i s P ri ce based on pag es Forewo rd BS0 (th e i n te rn ati o n al n ati on al Stan d ard s n ati on al Stan d ard s bod y i n terested ri g h t ts an d be is in a D raft carri e d represen ted I n tern ati o n al m e m be r th e I SO on bod i es h as in ou t for for l i s o n th rou g h a wi th I SO al so th e th ei r a take wsrl d wi d e werk of fed erati on d evel opi n g co m m i ttee s co m m i tte e I n tern ati on al by before is Th e tech n i cal tech n i cal I SO, ad opted apprsval Stan d ard fi n cl u d i n g in Fe bru ary been I SO d e ri vati ve s 3946 an d h as E ve ry been se t o rg an i zati on s, part te ch n i cal in th e as m e m be r h as th e wo rk co m m i tte e s acceptan ce up of I n ter- g ove rn m en tal are I n tern ati on al ci rcu l ate d to Stan d ard s by approved Arab bod y I n te rn ati o n al in d evel oped Qy- pro d u ctsl , by Te ch n i cal an d was Co m m i tte e ci rcu l ate d by th e m e m be r Rep of bo d i e s of th e fo l l o wi n g F R expre ssed Org an i zati o n N e th erl an d s U SA U SS R Swi tzerl an d Sou th d i sapproval for co u n tri es Rol an d P o rtu g al G e rm an y, m e m be r was I S O/TC to th e 982 Fran ce P ri n te d be d i es) C o u n ci l E g ypt, @ Stan d ard i zati on ) csm m i tte e Au stri a No for m e m be r wh i ch th at Stan d ard s bo d i e s I n tern ati on al Starch (60 su bj e ct n on - g ove rn m en tal , th e lt Org an i zati o n i n sti tu tes Afri ca, of th e S tan d ard i z ati o n , Rep sf d ocu m en t 982 : 93 , m e m ber I N TE RN ATI ON AL STAN D ARD S tarch es an d d eri ved ph o sph o ru s Scope Th i s fo r s tarch , % - s pe ci fi e s of d e ri vati ve s th e an d cal cu l ate d as - D eterm i n ati o n S pe ctro ph o to m etri c 4 appl i cati on S tan d ard co n te n t, of d e te rm i n ati o n i n cl u d i n g expe cte d e xce e d th e pro d u cts co n ten t fi el d I n te rn ati o n al m e th o d of an d I SO a to tal In s pe ctro ph o to m e tri c p h o s p h o ru s b y- pro d u cts , p h o s ph o ru s co n te n t in wh i ch th e (P) , d oes not a Ad d to (m l m ) I 500 co o l m o l ybd ate fl as k, d i s s o l ve S o l u ti o n , 0, g of [( N H &M o 70 ~ + 4H ml to of of (E ) to tal m eth o d Am m o n i u m te trah yd rate 46- 982 m o l /1 am bi e n t Sodi u m 46 P h o s ph o ru s , in s u l ph u ri c p re p are d as fo l l o ws am m o n i u m 500 aci d ml of m o l ybd ate wate r S o l u ti o n , mix an d al l o w te m pe ratu re h yd ro xi d e , m o l /1 S o l u ti o n D efi n i ti o n to tal ph o s p h o ru s d e te rm i n e d in I n te rn ati o n al p e rce n tag e co n te n t acco rd an ce S tan d ard by m ass : wi th an d of Th e th e q u an ti ty co n d i ti o n s e xp re s s e d th e p ro d u ct as as of s pe ci fi e d p h o s ph o ru s in (P) th i s as S to ck re ce i ve d We i g h , of s u l p h o - n i tri c th e o rg an i c m i xtu re an d s u bs tan ce s co n ve rs i o n by of d i g e s ti o n P h o s ph ate s wi th to a D i l u te Fo rm ati o n , by d ate as m e an s of a re d u ci n g m o l ybd e n u m S pe ctro ph o to m e tri c at to 00 mg of P per l i tre th e n e are s t 0, mg, 0, 439 d i h yd ro g e n o rth o p h o s ph ate q u an ti tati ve l y i n to a th e wate r an d 000 ml g of an h yd ro u s d i s s o l ve in o n e - m ark wate r vo l u m etri c ag e n t, of 825 ml of before of th e i n te n s i ty of th e th i s - N OTE a p h o s ph o m o l yb- bl u e m e as u re m e n t a wave l e n g th of to m ark wi th an d m i x o rth o - P h o s ph ate s co l o u r co rre s p o n d i n g fl as k P ri n ci pl e kn o w S o l u ti o n , to Tran s fe r D e s tru cti o n s o l u ti o n s a po tas s i u m S tan d ard ph o s p h o ru s S tan d ard po tas s i u m Th e u se fo r S o l u ti o n co o l i n a d ryi n g in a d e si ccator to S tan d ard 00 pg of P d i h yd rog en orth oph osph ate h, al l o wed co n tai n s oven co n tro l l e d at sh al l 05 + be d ri e d OC, an d th en bl u e nm S o l u ti o n , co rre s po n d i n g to mg of P per l i tre U si n g D u ri n g th e an al ys i s , g rad e , an d on l y u se d i s ti l l ed on l y re ag e n ts wate r of at of l e as t re co g n i z e d an al yti cal e q u i val e n t S u l ph o - n i tri c D i l u te ml ea , 84 by m i xi n g g /m l , aci d , Q~ 96 % p art (m l m ) by vo l u m e S o l u ti o n , an d of s u l ph u ri c N i tri t 38 As co rbi c Ke e p th i s aci d , g /m l , , 920 38 aci d , S o l u ti o n a 250 th e take ml m ark ml of o n e - m ark wi th th e s to ck vo l u m e tri c wate r an d S o l u ti o n (4 ) an d fl as k m i x th i s S tan d ard S o l u ti o n co n tai n s Mg of P p art by Apparatu s aci d , 65 % (m l m ) vo l u m e of in g /m l , g /l 65 % (m l m ) fo r E n su re th at th e d eterg en ts u sed for cl ean i n g g l assware n ot a m axi m u m of 48 ph osph oru s O rd i n ary S o l u ti o n S o l u ti o n a re fri g e rato r - S o l u ti o n tai n to of N OTE n i tri c pi pe tte , in re ag e n t P re pare d it pu ri ty a pl ace Reag en ts h I ab o rato ry O n e - m ark 200 - 250 I SO 42 an d ap paratu s , vo l u m e tri c 500 ml, an d in fl asks , co m pl yi n g parti cu l ar of wi th capaci ti e s th e 50 - 00 re q u i re m e n ts - of -Wed ce3 s0 f i Ph at m easu ri n g o pti cal patt- l wavel ee 7g tR of a ca”ry cwt th e pre paratrcm Qf; tkee a8 i brati oC n eß WQ an d H - Ed e l e rm i n ati o nwi ttai r-2 l i -i -j -ake 83al i q u o t w Ati d vd al e r 8Q easch m fh e %QVQD fl asks so th aak e h em taB vo l u n e i s appro xi m ate l y i n % T; X” P l ae e kh ese vend l asks in dx po rab-? (se? e @E? -tabkj i an d i n tro d u ce it im0 a n d i cal f-l ask bo i h n gwater baah5 fsr m i n U si n q th espectropp BOforQQl d el ermer, in th eeabsorban ace t Coo l arn bi e fi f cool i n g bash ti fati ve l y th e 8~ m arks U si n g he th e fl ask 50 by Tran sfer nd th e on e-rn ark wafe r an d of e ach wi th su l sf th e th e eu rve as = th e sarn pl e a fu n cfi n i m sn ersi n g th f3 csn ten ts of m h m e tai c fkks ahe fl asks in %I asks E ) th e %g z P h o rsu g h D y th e Ph e u si n g sd u ti on he sf d el m M -7~ ss~ u ti o n s, stan d ard g i vi n g Read f?- On n u m be r I 599, si x 825 n mQf- th isoi s L ! ti m i q u aw- = D i l u te r! - l i X~ spe ctroph obxaae te r nm cah brati on ph ospkaru s bl i x (5 5) tB - ~ wi ah th e P em peraQu re m m be r abssrban ee as he I d-I e caB i braai o cur1 bwQ m i crs g ram ci s f ph 0s ph 0- f I see th e csrre spon d i n QWS abso rban ce 0kh sr-i Ph e refe re n ce sf n - krog ram s frsm Plot of Carry o u t a pl aci n g U -e-i Carry ou t bI an k Test two B e st psrti o n in by d e terrn i rxh sn s d em v- n i n ati sn , paral l d PQ- wate r on th e sa- n e Test sam pl e (6 2) I SO E xpressi o n of resu l ts M e th o d Th e by total of cal cu l ati o n ph osph oru s m ass , is g i ve n by (PI th e an d ten t, d i fferen te between x V x 00 m x VI x 06 th e val u es obtai n ed in two d eterm i n a- fo rn wl a expre ssed as a ti o n s carri e d sam e test - ou t si m u l tan e o u sl y Sam pl e (6 21 , s h al l by n ot th e sam e an al yst on th e exceed percen tag e fo rm u l a % of ten ts ml (E l R e pe atabi l i ty Th e 464982 - th e i r ari th m e ti c g reater 0, 004 g of ph osph oru s th an m ean 0, % ph osph oru s ten ts per l ess in th e case of ph osph oru s (m l m ) ; th an 00 g 0, % of prod u ct in case s of (m l m ) wh ere m is th e m ass , ml is th e ph osph oru s So l u ti on read in fro m g ram s, of th e ten t, th e test in cal i brati o n P orti o n (6 ) ; m i cro g ram s , cu rve of th e test (6 ) ; V0 is th e d i l u ti on vol u m e, in m i l l i l i tre s , of th e test Test repo rt So l u ti on Th e test report s h al l i n d i cate th e m eth od u sed an d th e re s u l ts 5) ; obtai n ed n ot VI is th e vo l u m e , in m i l l i l i tre s , of th e al i q u ot P o rti on th e d eterm i n ati o n ad d i ti on , spe ci fi e d in th i s it s h al l m en ti o n I n tern ati on al an y operati n g Stan d ard , or d i ti on s reg ard ed as op- u sed ti on al , fo r In as we i l as an y ci rcu m stan ce s th at m ay h ave affected th e (6 6) re su l ts Take th e as th e two repeatabi l i ty re su l t th e ari th m e ti c d e te rm i n ati on s (7 2) are m ean provi d e d sati sfi e d lf n ot, of th at th e val u e s th e repeat obtai n ed d i ti on s th e in Th e of test test report i d en ti fi cati on sh al l of th e i n cl u d e al l d e tai l s req u i red Sam pl e Tal s i e E xpecte d ph osph oru s % ten t bl l m ) < 0, 05 M ass of test P o rti o n ( se c ) D i kti on wol u m e (se c 5) Vo l u m e of th e al i q u o t ( se c 6) g ml mi 0, 500 00 25 0, 05 to OJ O 0, 500 00 IO OJ O to 0, 25 0, 500 00 0, 25 to 0, 5O 0, 500 200 0, 50 to l , oo 0, 250 250 l , oo to 2, 00 0, 25 250 2 , OO to 5, 00 0, 25 500 P o rti o n for th e com pl ete This page intentionally left blank This page intentionally left blank This page intentionally left blank