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LE THUY LINH THAI NGUYEN UNIVERSITY UNIVERSITY OF AGRICULTURE AND FORESTRY LE THUY LINH ANTIMICROBIAL ACTIVITY OF ESCHERICHIA COLI USING N TiO2 PMMA COMPOSITE BACHELOR THESIS Study Mode Full time Majo[.]
THAI NGUYEN UNIVERSITY UNIVERSITY OF AGRICULTURE AND FORESTRY LE THUY LINH ANTIMICROBIAL ACTIVITY OF ESCHERICHIA COLI USING N-TiO2PMMA COMPOSITE BACHELOR THESIS Study Mode : Full-time Major: Environmental Science and Management Faculty: International Programs Office Batch : 2013-2017 Thai Nguyen, 2017 n n Thai Nguyen University of Agriculture and Forestry Degree Program Bachelor of Environmental Science and Management Student Name Le Thuy Linh Student ID DTN1353110171 Thesis Title Antimicrobial activity of E coli using N-TiO2-PMMA Composite Assoc Prof Nguyen The Hung Supervisor Prof Yao Tung-Lin Abstract: Titanium dioxide (TiO2) is a semiconductor and photocatalyst in the decomposition and disinfection of bacteria In particular, TiO2 were work under ultraviolet light and visible light It also had capable of producing a pair of electron holes due to exposure to ultraviolet and visible light From that Titanium dioxide can be used in combination with nitrogen (Nitrogen doped titanium dioxide) to create a substance that inhibits the activity and growth of bacteria in which clarify ability to inactivate, especially bacterial Escherichia Coli This experiment would use the dipcoating method with antibacterial by Nitrogen doped titanium dioxide (N-TiO2) and then saw the disinfection activity of E.coli for 24 hours by N-TiO2 0.5%, 1% and 2% During experiment took polymethyl methacrylate (PMMA) pieces dip-coating with NTiO2 and put PMMA under visible light with the amount 990 to 1010 lux In all experiments process, E.coli concentration was dilution by 104 and 105 CFU/ mL The results of the experiment showed that E.coli was affected by N-TiO2 antibacterial The number of growth and decomposition bacteria has a distinct gap when using Ni n TiO2with different percentages It would reduce the growth of bacteria and cough complete disinfection around 24 hours Antibacterial by N-TiO2 has showed as the optimum one to directly active on bacterial Titanium dioxide, Nitrogen doped titanium dioxide, Keywords Escherichia coli, Polymethyl Methacrylate Number of Pages 43 Date of Submission 20/09/2017 Supervisor’s signature ii n ACKNOWLEDGMENTS From bottom of my heart, I would like to express my deepest appreciation to all those who provided me the opportunity to complete this research First and foremost, I would like to express my sincere gratitude and deep regards to my supervisor: Pro Yao Tung-Ling of National Chung Hsing University, Taichung, Taiwan, who guided me wholeheartedly when I implemented this research He is always willing to give suggestions on scientific problems and personal issues He is very supportive and a good listener Under his instruction with patience, knowledge and talent, I’m able to fulfill to my thesis I also want to express my thanks to Assoc Prof Nguyen The Hung, the second supervisor, for his supervision, encouragement, advice, and guidance in writing this thesis In addition, formal thanks should be offered to the Rector of National Chung Hsing University for granting my internship acceptance I want to thank all of my colleagues in the ENM ( Environmental Nano Material) Laboratory, Miss Jing-Hua Tzeng, Mr Chakkrit Poonpakdee, Miss Li-Ting Yen, Miss Ya-Zhen Huang, Mr Le Quoc Anh, Miss Kai-Fen, Mr Che-Chien, Miss Than Thi Nhu Anh They are very generous and supportive It was a great pleasure to work with them I want to express my sincerest appreciation to my parents, my friends and my team for their love and support me everytime Thai Nguyen, 20thSeptember, 2017 Student Le Thuy Linh iii n TABLE OF CONTENT CHAPTER I: INTRODUCTION 1.1Introduction .1 1.2Objective of the study .3 CHAPTER II: Literiature Review 2.1 Bacteria species 2.2 Photocatalysis 2.3 Visible light responsive 2.4 N-TiO2 2.5 Polymethyl methacrylate ( PMMA) CHAPTER III: Material & methods 10 3.1 Material and equipment .11 3.2 Dip-coating of PMMA 14 3.3 Nutrient preparation .15 3.4 Antibacterial experiment 16 3.4.1 Dosage bacteria without N-TiO2 16 CHAPTER IV: Result &Discussion 19 4.1 Control experiment to E.coli 19 4.2 N-TiO2 dosage effect 21 4.3 Compare N-TiO2 dosage effect .34 4.4 Compare control and N-TiO2 dosage effect 37 4.5 Compare S.aureus and E.coli .38 CHAPTER V: Conclusion .40 Reference : .41 iv n LIST OF TABLES Table 1: The bacteria used in experiment…………………………………………… 11 Table 2: Some of nutrient used in experiment……………………………………… 11 Table 3: Some of chemical used in experiment………………………………………12 Table 4: Some of equipments used in experiment ……………………………………13 v n LIST OF FIGURES Figure 1: Bacterial Escherichia coli ( E.coli)………………………………………….4 Figure 2: The Polymethyl methacrylate plastic ( PMMA)……………………………8 Figure 3: Flow chart of work…………………………………………………………10 Figure 4:Show the Dip-coating process…………………………………………… 15 Figure 5:Dosage effect without N-TiO2…………………………………………… 19 Figure 6: Another control experiment……………………………………………… 20 Figure7: N-TiO2 0.5% dosage effect with visible light intensity 2.2x10-4 lux: a, survival of bacteria ; b amount of bacteria………………………………………… 22 Figure 8:N-TiO2 0.5% dosage effect with visible light intensity 3.09x10-4 lux: a, survival of bacteria ; b amount of bacteria………………………………………… 22 Figure 9: N-TiO2 0.5% dosage effect with visible light intensity 1019 lux: a, survival of bacteria; b amount of bacteria…………………………………………………….23 Figure 10:N -TiO2 1% dosage effect with visible light intensity 2.91x10-4-1006 lux: a, survival or bacterial; b, amount of bacterial………………………………………… 25 Figure 11:N-TiO2 1% dosage effect with visible light intensity 2.95x10-4, 1000 lux in Box A: a, survival or bacterial; b, amount of bacterial……………………………….26 Figure 12:N-TiO2 1% dosage effect with visible light intensity 2.95x10-4, 1000 lux in Box B: a, survival or bacterial; b, amount of bacterial……………………………….27 vi n Figure 13:N-TiO2 1% dosage effect with visible light intensity 1004 lux : a, survival or bacterial; b, amount of bacterial……………………………………………………28 Figure 14:N-TiO2 1% dosage effect with visible light intensity 1007 lux: a, survival or bacterial; b, amount of bacterial………………………………………………… 29 Figure 15:N-TiO2 2% dosage effect with visible light intensity 2.96x10-4- 1007 lux in Box A: a, survival or bacterial; b, amount of bacterial……………………………….30 Figure 16: N-TiO2 2% dosage effect with visible light intensity 2.95x10-4-1000 lux in Box B: a, survival or bacterial; b, amount of bacterial……………………………….31 Figure 17: N-TiO2 2% dosage effect with visible light intensity 1005lux in Box A: a, survival or bacterial; b, amount of bacterial………………………………………….32 Figure 18: N-TiO2 2% dosage effect with visible light intensity 1005 lux in Box A: a, survival or bacterial; b, amount of bacterial……………………………………… 33 Figure 19: Compare N-TiO2 dosage effect: a, survival or bacterial; b, amount of bacterial……………………………………………………………………………….35 Figure 20: N-TiO2 dosage effect to S.aureus: a, survival or bacterial; b, amount of bacterial……………………………………………………………………………….36 vii n n