LAB REPORT 4 Enumeration of TOTAL LIPID IN SOLID SAMPLES BY SOXLET METHOD 1 Goals of Practice Determination of lipids in raw materials or solid food products by extraction with organic solvents 2 Prin[.]
LAB REPORT Enumeration of TOTAL LIPID IN SOLID SAMPLES BY SOXLET METHOD 1.Goals of Practice Determination of lipids in raw materials or solid food products by extraction with organic solvents 2.Principle of Method Calculated by directly weighing the oil after distillation or removing the solvent by the mass remaining after the complete extraction of the lipids with the solvent 3.Implementation process Weigh about 2-3g of sample and place in Soxhlet flask Prepare the extraction solution by mixing hydrochloric acid (HCl) and ethanol in a ratio of 3:2 Heat the Soxhlet flask with the extraction solution in step The extract will flow through the solid sample, dissolve the lipids, and filter through the concentrator Use a special concentrator to concentrate the extract and obtain lipids Dry the lipids at high temperature to ensure all residual water is removed Accurately weigh the resulting lipids Calculate the amount of lipid in the solid model based on the obtained lipid mass and the initial solid sample mass Solid pattern Paper wrapping Suspect For solvent Turn on the heat source Wet sample Put in the pillar of philosophy Refrigerate the birth boy Extracts Wait for it to dry Drying Weighing constant mass 4.Result Calculate The fat content (%) is calculated according to the formula X=(A-B)*100/C In there A is the mass of the paper bag and the original sample (g) B is the mass of the paper bag and sample after lipid extraction and drying (g) C is the initial sample mass (g) Sample Rep Initial weight (g) Wet sample (g) Dried sample (g) %Moisture Soy bean meal type 1 3.0016 3.0068 2.3502 21.8371 3.0112 3.0066 2.3331 22.4007 3.0059 3.0014 2.2928 23.6090 Mean = 22.6156 SD = 0.9053 Rep %Fat + % Moisture Cal.%Fat 21.9902 0.1597 22.5537 0.1530 23.7589 0.1499 Sample Rep Initial weight (g) Wet sample (g) Dried sample (g) %Moisture Soy bean meal type 3.0122 3.0070 2.3311 22.4776 3.0114 3.0066 2.2809 24.1369 3.0012 3.0065 2.1799 27.4938 Mean = 24.7028 SD = 2.556 5.Discuss Advantage: High Accuracy: The Soxlet method provides high accuracy results for the quantification of total lipids in solid samples Good repeatability: The Soxlet method allows to achieve good repeatability, as the extraction and extraction steps are performed multiple times to ensure accuracy Time saving: The Soxlet method allows to process several samples at once, saving time compared to other methods Ease of implementation: The Soxlet method is a simple and easy to implement method that does not require complex technical skills Disadvantage: Solvents required: The Soxlet method uses a lot of solvents, which are costly and harmful to the environment Ask questions about cash registers: The Soxlet method requires the use of cash registers such as the Soxlet suite, making the cost of implementing this method higher than other methods Possible lipid loss: During lipid extraction and separation, lipid loss may occur, affecting the quantitative results Difficulty in handling insoluble samples: If the solid sample has insoluble properties