sealbio a novel non obturation endodontic treatment based on concept of regeneration

Original Article SealBio: A novel, non-obturation endodontic treatment based on concept of regeneration Naseem Shah, Ajay Logani Department of Conservative Dentistry and Endodontics and Centre for Dental Education and Research, All India Institute of Medical Sciences, New Delhi, India Abstract Introduction “SealBio”, an innovative, non-surgical endodontic treatment protocol, based on “regenerative concept” has Introduction: been developed to manage pulp and periapically involved teeth Materials and Methods Methods: Subsequent to Institute’s ethical clearance, 18 patients presenting with signs and symptoms of pulp and periapical disease were included in the study (11/M, 7/F; Mean age - 44.7 years; range 15-76 years) The protocol included a modified cleaning and shaping technique involving apical clearing and foramen widening, combined with inducing bleeding and clot formation in the apical region Calcium-sulphate based cement was condensed with hand pluggers into the canal orifices An appropriate permanent restoration was given The patients were followed-up clinically and radiographically at regular interval of months Six teeth in patients were also evaluated pre and post treatment CBCT at 6-months Results The novel treatment protocol was found to be favourable in resolving periapical infection, both clinically and Results: radiographically Conclusions This innovative endodontic treatment protocol highlights and reiterates the importance of cleaning and shaping Conclusions: and puts forth the possible role of stem cells and growth factors in healing after non-surgical endodontic therapy Keywords: CBCT; non-obturation Endodontic treatment; periapical healing; regenerative endodontics; sealBio INTRODUCTION One of the essential requisites for successful outcome of endodontic treatment is believed to be achieving sealing of root canal system at both; apical and coronal end of a disinfected root canal Even with conventional guttapercha obturation, the ultimate aim is to achieve a cemental/ fibrous barrier at the root apex.[1] Therefore, achieving a biological seal, “SealBio” should be preferable over an artificial barrier of sealer and guttapercha cones at the apical end of root canal system With the success achieved with “revascularization” in healing of periapical lesions and hard tissue deposition at apical and lateral walls of the root canals (maturogenesis) in immature teeth,[2-4] the processes involved in the healing mechanism are now better understood Role of various stem cells, growth factors, Hertwig’s epithelial root sheath (HERS) and their interactions in regeneration of tissues have been documented.[5] Exploiting this mechanism of stimulating healing and regeneration of tissues and combining it with thorough disinfection of root canal system, a novel treatment approach was conceived to manage non-vital mature teeth with periapical pathology MATERIALS AND METHODS Institutional ethical clearance was obtained Eighteen cases of pulp and periapical infection, irrespective of age, gender or the tooth involved were included in the study (11 males, females; age range, 15-76 years; mean age, 44.7 years) The cases presented with acute or chronic apical abscess, with or without radiographic evidence of periapical pathology After written informed consent of the patient, access opening, cleaning and shaping of root canals by crowndown technique was done The canals were copiously irrigated with 2.5% sodium hypochlorite Depending on the Address for correspondence: Dr Naseem Shah, Department of Conservative Dentistry and Endodontics and Chief, Centre for Dental Education and Research, All India Institute of Medical Sciences, New Delhi- 110 029, India E-mail: naseemys@gmail.com Access this article online Quick Response Code: Date of submission : 07.04.2012 Review completed : 16.06.2012 Date of acceptance : 19.07.2012 328 Journal of Conservative Dentistry | Oct-Dec 2012 | Vol 15 | Issue Website: www.jcd.org.in DOI: 10.4103/0972-0707.101889 Shah and Logani: Sealbio: Non-obturation endodontic treatment extent of infection; either one or two inter-appointment dressing of triple antibiotic paste of metrogyl, ciprofloxacin and tetracycline was given Special attention was given to apical third cleaning Apical patency was maintained throughout the cleaning and shaping procedure The apical third of the canal was debrided by “apical clearing” which involved its enlargement with 2-4 file sizes larger than the master apical file (MAF) at working length and removal of loose debris from the apical region.[6] Subsequent to this “apical foramen widening” was done with larger K-files used sequentially till size 25-30 to clean the cemental part of the canals When the infection control was achieved, as evident from a clinically symptom-free tooth, healed swelling or sinuses, a final wash with an anti-microbial solution was done and the canals were dried After checking the patency, determined by smooth passage of # 15 ISO instrument, intentional over-instrumentation into periapical region was done with #20 K-file to induce bleeding near the apical foramen The file was gently given 2-3 clock-wise turns and then withdrawn by giving counterclockwise rotation A calcium sulphate-based cement (Cavit) was introduced in the access cavity and with a hand plugger, condensed into the cervical third of root canals A suitable coronal restoration was given Immediate posttreatment radiograph was taken The patient was recalled every 6-months for clinical and radiographic evaluation Pre and post treatment CBCT was done for six teeth in cases who volunteered to undergo CBCT evaluation All the cases were done on a single iCAT machine at 120 kvp, mA with an exposure time of seconds and voxel size of 0.25 The parameters evaluated were: lesion size, bone and cementum density in HU and periapical index (CBCT-PAI) and disinfection of root canals, peri-radicular lesion had healed even without obturation of root canal.[8] Dubrow[9] in 1976 had argued that often treatment failures that were attributed to poor obturation could be the result of improper debridement He also suggested that if canals were thoroughly debrided, healing of periapical tissues would occur and tissue fluid may not enter the canal space, even if it was not obturated Dass[10] commenting on calcium hydroxide apexification had also stated that “perhaps filling is unessential and mere eradication of infection may be sufficient for apexification.” Role of thorough canal debridement and disinfection in periapical healing with incomplete obturation of root canals has been reported [11] In an experimental animal study on dog’s teeth reported in 2006, it was documented that there was no difference in healing of apical periodontitis with and without obturation, if root canals were thoroughly instrumented and debrided [12] In the recent past, the new treatment method of inducing revascularization in non-vital immature teeth has shown very encouraging results.[2-4] The present study was planned to determine whether a novel, non-surgical treatment protocol, based on regenerative principles, can be effective in fully mature teeth with pulp and periapical infection The argument given for complete 3-diemensional, fluid tight seal of the entire root canal system is that it will entomb the remaining microbes and also will not permit or at least delay the microbial infiltration, if there was leakage from the coronal restoration.[13] However, even after complete obturation, if coronal seal is broken for more than RESULTS All the 18 cases treated by this novel endodontic treatment protocol showed very good response; both clinically as well as radiographically Follow-up of years was completed for cases, ½ years for cases,;2 years for cases and 6-months for cases [Figures and 2] The soft tissue healing was excellent; intraoral sinus, buccal soft tissue swelling and bone expansion had completely resolved in all the cases On radiographic examination, complete resolution or decrease in the size of periapical lesion was evident In two cases of endo-perio lesion, marked healing of the periodontal defect was also seen The CBCT findings are shown in Table Remarkable decrease in the lesion size (CBCT-PAI) and increase in bone and cementum density {in Hounsfield unit (HU)} were documented [Figures and 4] DISCUSSION The basis for success of endodontic treatment is to remove the cause, i.e all necrotic debris, bacteria and their by products As early as in 1939, it was known that the root canal was the seat of infection.[7] After debridement a b c d Figure 1: (a) A 53-year-old man presented with discrete soft tissue swellings at gingival margin in relation to # 47 (b) Sinus tract traced with guttapercha point documenting a pulpoperiodontal lesion Note the bone loss on mesial aspect of mesial root (c) Follow up X-ray at months showing healing of periodontal defect (d) At 3-years, complete healing and new bone regeneration in inter dental area between #46 and #47 is evident Journal of Conservative Dentistry | Oct-Dec 2012 | Vol 15 | Issue 329 Shah and Logani: Sealbio: Non-obturation endodontic treatment a b c Figure 2: (a) Immediate post-treatment X-ray showing diffuse radiolucency around distal root in a 40-year-old man (b) Just 6-weeks after the treatment, remarkable healing of the lesion is seen (c) One-year follow-up x-ray showing complete healing Figure 3: CBCT scan (i) & (ii) showing the pre and 6-months post treatment result Figure 4: CBCT scan (i) & (ii) showing the pre and 6-months post treatment result Table 1: Showing result of CBCT evaluation of teeth in cases Case Tooth Tooth #36 Tooth #37 Tooth #46 Tooth #47 Tooth #36 Tooth #36 Root Mesial root Distal root Mesial root Distal root Mesial root Distal root Mesial root Distal root Mesial root Distal root Mesial root Distal root Lesion size HU bone CBCT-PAI Follow-up Initial Follow-up Initial Follow-up Initial Follow-up 8.98 5.20 4.55 4.14 5.53 5.75 7.28 5.06 4.76 9.01 2.02 3.35 7.6 5.10 3.64 3.69 4.76 5.50 3.40 3.91 1.90 5.02 1.0 1.35 288 631 443 343 206 518 531 145 697 422 308 606 341 749 764 630 908 1131 716 383 782 674 463 805 64 154 133 49 84 159 53 52 87 82 169 255 96 284 223 53 498 693 53 92 275 197 1122 961 4 4 4 4+D 5+D 4 3 4 3 3-4 weeks, it is recommended to perform retreatment, as during this period, bacteria would have colonized the pulp space and the tooth would develop endodontic infection sooner or later.[14] Grossman in 1953 had stated that an optimal concentration of necrotic debris or toxic load is necessary to sustain or increase the periapical lesion.[15] It has also been concluded based on clinical and experimental animal studies that stagnant tissue fluid and sterile necrotic pulp tissue not sustain inflammation at the periapex.[16] Fabricius[17] had stated that permanent root canal filling, per se, had a limited effect on the outcome of endodontic 330 HU cementum Initial treatment, if bacterial load was not controlled at the time of obturation This exhaustive animal study adequately underlined the importance of thorough disinfection of canals rather than depend on high quality obturation for favorable outcome of endodontic treatment Siqueria[18] put forth the argument that for any species to cause disease, they have to reach a population density (load) conducive to cause tissue damage, either directly or by host tissue response to infection Hence, clinically it is essential to reduce bacterial load to levels below that detected by culture procedures, i.e.103-104 cells The treatment protocols should be so standardized that the bacterial count is brought to below this known threshold Journal of Conservative Dentistry | Oct-Dec 2012 | Vol 15 | Issue Shah and Logani: Sealbio: Non-obturation endodontic treatment In the present study, heavy stress was laid on thorough disinfection of canal space and tight coronal seal Special care was taken to clean the apical third of the canal space Apical clearing, apical foramen widening and overinstrumentation into periapical region were done to induce bleeding near apical foramen It is hypothesized that the clot formed provides a scaffold into which locally residing stem cells can get seeded and the cascade of healing process can initiate “Apical clearing” is a technique described to remove loose debris from the apical region by widening the apical canal with instruments 2-4 sizes larger than the master apical file (MAF) used at radiographic terminus without transportation of the canal or the apical foramen.[19] It is documented that in cases of apical periodontitis, intra-canal bacteria can penetrate dentin to a depth of 150-250 μ, where they remain protected from the action of medicament and irrigants.[20] Therefore, apical canal widening to 300-500 μ is required to thoroughly cleanse the apical portion of the canal Apical foramen widening was done with gradually increasing number of files till no #25 or #30 This allowed thorough cleaning of cemental part of the canal and also ensured subsequent smooth passage of instrument taken past the foramen without breakage Intentional over-instrumentation past the apical foramen into the periapical tissues contributes towards healing of the lesion as well as towards achieving biological barrier of hard and soft tissues as seen in cases of revascularization of immature teeth This method of over-instrumentation was found very effective in resolution of periapical pathology in a prospective clinical study[21] by the first author in 1988 Various stem cells reside in the periapical region of teeth such as periodontal ligament stem cells (SCPDL), dental pulp stem cells (DPSC), bone marrow mesenchymal stem cells (BMMSC) and more recently identified stem cells from apical papilla (SCAP) These cells are now documented to play a role in maturogenesis of immature teeth using revascularization procedure.[22] It could be hypothesized that the same mechanism probably takes place in cases of mature teeth; the bleeding and clot formed in the area of apical foramen by over-instrumentation can lead to seeding of stem cells, their proliferation, differentiation and mineralized tissue formation, sealing the apical foramen The importance of a tight coronal seal cannot be overemphasized Without a coronal seal, bacteria and bacterial toxins can reach the apex through an obturated canal in just 20 days.[23, 24] Cavit, a zinc-sulphate based material was chosen to seal the canal orifices It served two purposes: (i) calcium sulphate cement is documented to have very good sealing properties[25] and (ii) in case retreatment was required, it would be easier to remove from canal orifices as compared to glass ionomer cement or mineral trioxide aggregate Care was exercised in planning and placement of coronal restoration Although it has been suggested that reversal of healing process after non-surgical endodontic treatment is uncommon[26] and hence prolonged follow-up of cases showing healing may not be necessary.[27] Nevertheless, in the present study, a maximum follow-up of three years was possible and the cases are planned to be followed-up further The role of CBCT in evaluation of periapical healing has been reported.[28] It provides objective, non-invasive method to measure periapical healing and mineralized tissue deposition CBCT evaluation of six teeth showed increased density of bone and cementum, supporting the hypothesis that mineralized barrier following this novel technique does take place Metzger Z and Abramowitz[29, 30] reflecting on philosophy of endodontic treatment commented that “cleaning, shaping and preventing bacterial stimulation at root apices was taking a simplistic and mechanistic view Endodontics should not limit itself to only better ways of cleaning, shaping and obturation New concepts and methods, based on biological concepts must supplement traditional ones” Tronstad also suggested the possibility of “Biological Obturation Technique” in future The present innovative technique utilizing modified cleaning and shaping technique combined with regenerative, tissue engineering principles for treatment of infected, non-vital mature teeth is a step in this direction Further well-planned experimental animal studies can provide information on type of cells involved and tissue deposited The evidence generated from this case-series also questions the role of obturation in non-surgical endodontic treatment However, again, more evidence would need to be generated in the form of a randomized clinical trial to evaluate the role of obturation in view of recent advances in disinfection protocols, techniques and restorative materials CONCLUSION The new technique, as discussed and documented in this pilot clinical study can prove to be the most simple, easy to perform and cost-effective method of regenerative endodontic treatment The result proves the importance of thorough cleaning and shaping and a well-condensed, bacteria-tight coronal seal in endodontic treatment success A small sub-set of six teeth evaluated by pre and post treatment CBCT has shown increased density of bone and cementum, supporting the premise that mineralized tissue deposition does occur at the root apex (hence sealing the apical end with biological tissue, i.e SealBio) Journal of Conservative Dentistry | Oct-Dec 2012 | Vol 15 | Issue 331 Shah and Logani: Sealbio: Non-obturation endodontic treatment ACKNOWLEDGMENTS We wish to thank the administration and ethics committee of the All India Institute of Medical Science, New Delhi for permitting us to undertake this study We also would like to acknowledge all the postgraduate students of the department of Conservative Dentistry and Endodontics for their support during the period of the study 14 15 16 17 18 DISCLAIMER 19 The views and opinion reported in this manuscript does not necessarily reflect the clinical standard of care policies of J Conserv Dent / IACDE 20 REFERENCES 21 10 11 12 13 Voin ovich O, Voinovich J Periodontal cell migration into the apical pulp during the repair process after pulpectomy in immature teeth: An auto radiographic study J Oral Rehabil 1993;20:637-52 Iwaya S, Ikawa M, Kubota M Revascularization of an immature permanent tooth with apical periodontitis and sinus tract Dent Traumatol 2001;17:185-7 Banchs F, Trope M Revascularization of immature permanent teeth with apical periodontitis: New treatment protocol? J Endod 2004;30:196-200 Shah N, Logani A, Bhasker U, Aggarwal V Revascularization for inducing apexogenesis/apexification in non-vital, immature permanent incisors: A pilot study J Endod 2007;34:919-25 Matusow RJ Acute pulpal-alveolar cellulitis syndrome V Apical closure of immature teeth by infection control: Case report and a possible microbial-immunologic etiology Part Oral Surg Oral Med Oral Pathol 1991;71:737-42 Parris J, Wilcox L, Walton R Effectiveness of apical clearing: Histological and radiographical evaluation J Endod 1994;20:219-24 Fish Bone infections J Amer J Dent Assoc 1939;26:691 Ingle JI, Bakland LK, Baumgartner JC Endodontics 6- ed Ontario: B C Decker Inc; 2008 p 922 Dubrow H Silver points and guttapercha and the role of root canal fillings J Am Dent Assoc 1976;93:976-80 Das S Apexification in a non-vital tooth by control of infection J Am Dent Assoc 1980;100:880-1 Szajkis S, Taggar M Periapical healing in spite of incomplete root canal debridement and filling J Endod 1983;9:203-9 Sabeti MA, Nekofar M, Motabbary P, Ghandi M, Simon JH Healing of apical periodontitis after endodontic treatment with and without obturation in dogs J Endod 2006;32:628-33 Saunders WP, Saunders EM Coronal leakage as a cause of failure in 22 23 24 25 26 27 28 29 30 root-canal therapy: A review Endod Dent Traumatol 1994;10:105-8 Khayat A, Lee SJ, Torabinejad M Human saliva penetration of coronally unsealed obturated root canals J Endod 1993;19:458-61 Grossman LI Antibiotics vs instrumentation A reply N Y State Dent J 1953;19:409 Klevant FJ, Eggink CO The effect of canal preparation on periapical disease Int Endod J 1983;16:68-75 Fabricius L, Dahlén G, Sundqvist G, Happonen RP, Möller AJ Influence of residual bacteria on periapical tissue healing after chemo-mechanical treatment and root filling of experimentally infected monkey teeth Eur J Oral Sci 2006;114:278-85 Siqueria JF Jr, Rocas IN Clinical implications and microbiology of bacterial persistence after treatment procedures J Endod 2008;34:1291-130 Card SJ, Sigurdsson A, Orstavik D, Trope M The effectiveness of increased apical enlargement in reducing intra-canal bacteria J Endod 2002;28:779-83 Borlina SC, de Souza V, Holland R, Murata SS, Gomes-Filho JE, Dezan Junior E, et al Influence of apical foramen widening and sealer on the healing of chronic periapical lesions induced in dogs’ teeth Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010;109:932-40 Shah N Non-Surgical management of periapical lesions: A prospective Study Oral Surg Oral Med Oral Pathol 1988;66:3665-371 Gronthos S, Mankani M, Brahim J, Robey PG, Shi S Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo Proc Natl Acad Sci U S A 2000;7:13625-30 Heithersay GS Stimulation of root formation in incompletely developed pulpless teeth Oral Surg Oral Med Oral Pathol 1970;29:620-30 Beach CW, Calhoun JC, Bramwell JD, Hutter JW, Miller GA Clinical evaluation of bacterial leakage of endodontic temporary filling materials J Endod 1996;22:459-62 Vail MM, Steffel CI Preference of temporary restorations and spacers: A survey of Diplomate of the American Board of Endodontics J Endod 2006;32:513-5 Orstavik D Time course and risk analysis of the development and healing of chronic apical periodontitis in man Int Endod J 1996;29:150- Kvist T, Reit C Results of Endodontic retreatment: A randomized clinical study comparing surgical and non-surgical procedures J Endod 1999;25:814-7 Estrela C, Bueno MR, Azevedo BC, Azevedo JR, Pécora JD A new periapical index based on cone beam computed tomography J Endod 2008;34:1325-31 Metzger Z, Abramovitz I Periapical lesions of endodontic origin In: Ingle JI, Bakland LK, Baumgartner JC, editors Ingle’s Endodontics 6th ed Ontario; BC Decker Inc 2008 p 50 Tronstad L Clinical Endodontics: A Textbook 2nd revised ed New York: Thieme Stuttgart; 2003 p 200 How to cite this article: Shah N, Logani A SealBio: A novel, nonobturation endodontic treatment based on concept of regeneration J Conserv Dent 2012;15:328-32 Source of Support: Nil, Conflict of Interest: None declared Staying in touch with the journal 1) Table of Contents (TOC) email alert Receive an email alert containing the TOC when a new complete issue of the journal is made available online To register for TOC alerts go to www.jcd.org.in/signup.asp 2) RSS feeds Really Simple Syndication (RSS) helps you to get alerts on new publication right on your desktop without going to the journal’s website You need a software (e.g RSSReader, Feed Demon, FeedReader, My Yahoo!, NewsGator and NewzCrawler) to get advantage of this tool RSS feeds can also be read through FireFox or Microsoft Outlook 2007 Once any of these small (and mostly free) software is installed, add www jcd.org.in/rssfeed.asp as one of the feeds 332 Journal of Conservative Dentistry | Oct-Dec 2012 | Vol 15 | Issue