skin microbiome and acne vulgaris staphylococcus a new actor in acne

Accepted Article MRS SOPHIE SEITE (Orcid ID : 0000-0001-9335-4139) Received Date : 07-Jul-2016 Revised Date : 16-Dec-2016 Accepted Date : 28-Dec-2016 Article type : Regular Article Title: SKIN MICROBIOME AND ACNE VULGARIS: STAPHYLOCOCCUS, A NEW ACTOR IN ACNE Short title: Skin microbiome in acne vulgaris Authors : Brigitte Dreno1-2, Richard Martin3, Dominique Moyal4, Jessica B Henley4, Amir Khammari1-2 and Sophie Seité5 Department of Dermatology, Nantes University Hospital, 44093 Nantes, France CIC, Inserm U892-CNRS 6299, 44093 Nantes, France L’Oréal Research and Innovation, Tours, France Cooperative Institute for Research in Environmental Sciences, University of Colorado, Boulder, Colorado, USA La Roche-Posay Dermatological Laboratories, Asnières, France Corresponding author: Sophie Seité, Ph D., La Roche-Posay Dermatological Laboratories, 110 Avenue Henri Barbusse, 92600 Asnières, France Phone: (33)1.46.88.65.44; Fax: (33) 1.46.88.66.88 E-mail: sophie.seite@loreal.com Conflict of Interest: This study was funded by La Roche-Posay Dermatological Laboratories This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record Please cite this article as doi: 10.1111/exd.13296 This article is protected by copyright All rights reserved Accepted Article Martin R, Moyal D and Seité S are employees of L’Oréal Dréno B, Khammari A and Henley JB have no interest to disclose Statistics Abstract: 247 words Core Text: 3189 words Tables: table +4 additional tables Figures: figures + additional figure References: 40 ABSTRACT Propionibacterium acnes (P acnes), the sebaceous gland and follicular keratinocytes are considered the three actors involved in the development of acne This exploratory study investigated the characteristics of the skin microbiota in subjects with acne and determined microbiota changes after 28 days of application of erythromycin 4% or a dermocosmetic Skin Microbiota were collected under axenic conditions from comedones, papulo-pustular lesions and nonlesional skin areas from subjects with mild to moderate acne according to the GEA Grading using swabs Samples were characterised using a high-throughput sequencing approach that targets a portion of the bacterial 16S rRNA gene Results Overall, microbiota samples from 26 subjects showed an overabundance of Proteobacteria and Firmicutes and an underrepresentation of Actinobacteria Staphylococci were more abundant on the surface of comedones, papules and pustules (p=0.004 and p=0.003 respectively) than on nonlesional skin Their proportions increased significantly with acne severity (p