rna sequencing of chorionic villi from recurrent pregnancy loss patients reveals impaired function of basic nuclear and cellular machinery

www.nature.com/scientificreports OPEN received: 04 August 2016 accepted: 08 November 2016 Published: 08 December 2016 RNA sequencing of chorionic villi from recurrent pregnancy loss patients reveals impaired function of basic nuclear and cellular machinery Siim Sõber1,*, Kristiina Rull1,2,3,*, Mario Reiman1, Piret Ilisson4, Pirkko Mattila5,6 & Maris Laan1,7 Recurrent pregnancy loss (RPL) concerns ~3% of couples aiming at childbirth In the current study, transcriptomes and miRNomes of 1st trimester placental chorionic villi were analysed for RPL cases (≥6 miscarriages) and normal, but electively terminated pregnancies (ETP; n = 8) Sequencing was performed on Illumina HiSeq 2000 platform Differential expression analyses detected 51 (27%) transcripts with increased and 138 (73%) with decreased expression in RPL compared to ETP (DESeq: FDR P