Phospholipases Dα and δ are involved in local and systemic wound responses of cotton (G. hirsutum)
Introduction
Materials and methods
Plant material and stress treatment
PLD activity isolation
PLDα activity assay and transphosphatidylation reaction
Lipid extraction
Lipid analysis
RNA isolation and cDNA preparation
Quantitative Real‐Time PCR and HRM
Results
G. hirsutum PLDα activity is localized in the microsomal fraction
Wounding of cotton leaves results in both local and systemic increase of PLDα activity
Phospholipid determination reveals different PtdOH production pattern during local and systemic responses and differential consumption of PLD endogenous substrates
Wounding differentially upregulates PLDα and PLDδ expression during local and systemic responses