Polarization of M2 macrophages requires Lamtor1 that integrates cytokine and amino acid signals Erratum Polarization of M2 macrophages requires Lamtor1 that integrates cytokine and amino acid signals[.]
DOI: 10.1038/ncomms14711 OPEN Erratum: Polarization of M2 macrophages requires Lamtor1 that integrates cytokine and amino-acid signals Tetsuya Kimura, Shigeyuki Nada, Noriko Takegahara, Tatsusada Okuno, Satoshi Nojima, Sujin Kang, Daisuke Ito, Keiko Morimoto, Takashi Hosokawa, Yoshitomo Hayama, Yuichi Mitsui, Natsuki Sakurai, Hana Sarashina-Kida, Masayuki Nishide, Yohei Maeda, Hyota Takamatsu, Daisuke Okuzaki, Masaki Yamada, Masato Okada & Atsushi Kumanogoh Nature Communications 7:13130 doi: 10.1038/ncomms13130 (2016); Published 12 Oct 2016; Updated 27 Feb 2017 In this Article, there are errors in the labelling of the x axis in Fig 5g,h that were introduced during the production process The second label on the x axis of each graph in Fig 5g should have been ‘FWT’ instead of ‘FKO’ In Fig 5h, the labels ‘0’, ’5’, ’10’ and ‘0’ should have been ‘0‘, ‘1’, ’5’ and ‘10’, respectively In addition, lane labels indicating the duration of leucine stimulation are missing from the Western blot in Fig 5b, and should have read ‘0’, ‘5’, ‘10’, ‘15’, ‘20,’ ‘0’, ‘5’, ‘10’, ‘15’ and ‘20’, from left to right The correct version of Fig appears below as Fig NATURE COMMUNICATIONS | 8:14711 | DOI: 10.1038/ncomms14711 | www.nature.com/naturecommunications ERRATUM NATURE COMMUNICATIONS | DOI: 10.1038/ncomms14711 a b Amino acids: – – + + + + + + + – + – ΦWT ΦKO ΦWT ΦKO ΦWT BMDMs: ΦKO p-S6K S6K d e 0.5 0.5 ** – + Amino acid starvation 0.5 A.U **1.0 ** ** 0.5 0.5 A.U 0.5 0.5 Baf (ng/ml) 10 30 + + + + + 10 15 20 70 kDa 0.4 1.0 1.1 1.1 70 kDa h *** *** *** ΦWT ΦWT ΦWT ΦKO 10 ΦWT ΦWT ΦKO 0 10 *** *** *** 1.0 0.5 10 30 10 30 IL-4, days l m + 400 – p-S6K 70 kDa S6K 70 kDa A.U Glucose (mg dl–1): 400 50 10 Amino acids: + + + 1.0 *** *** *** 1.0 0.5 0.5 *** *** *** 0 ConA (ng/ml) j RELMα ** * ** 0 IL-6 iNOS 10 10 IFNγ + LPS, days IL-4, days – – – – 10 15 20 S6K ΦWT IL10 *** *** *** 1.0 – 1.0 1.9 2.1 2.3 RELMα 1.0 Arg1 1.0 70 kDa IL-4, day 18 kDa IFNγ + LPS, days 0 BMDMs: ΦWT ΦWT ΦWT ΦKO ConA 10 (ng/ml) i 70 kDa ConA (pretreatment) – + Amino acid starvation IL10 0.5 3.3 70 kDa Leucine (min) Arg1 1.0 0.3 1.1 p-S6K * – + Amino acid starvation *** *** *** 70 kDa f NS IL-4, days g Amino acids p-S6K S6K 10 15 20 iNOS 1.0 1.0 A.U A.U Leucine (min) 10 15 20 p-S6K 5.5 6.1 6.6 70 kDa S6K 70 kDa Lamtor1 IL10 Arg1 1.0 c ΦKO ΦWT BMDMs: A.U IL-4 (2 hours): iNOS 1.0 *** * *** 1.0 0.5 0.5 *** * *** IL-4 (20 min): ConA (ng/ml): Torin1 (nM): – 0 + 0 + + 10 0 250 p-STAT6 100 kDa STAT6 100 kDa 0 Baf (ng/ml) 10 30 10 30 IFNγ + LPS, days RELMα IL10 Arg1 1.0 1.0 1.0 0.5 0.5 0.5 k IL-6 400 50 10 Glucose (mg dl–1) 0 400 50 10 Glucose (mg dl–1) 400 50 10 Glucose (mg dl–1) IL-4, days Figure | This work is licensed under a Creative Commons Attribution 4.0 International License The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ r The Author(s) 2017 NATURE COMMUNICATIONS | 8:14711 | DOI: 10.1038/ncomms14711 | www.nature.com/naturecommunications ... ConA (pretreatment) – + Amino acid starvation IL10 0.5 3.3 70 kDa Leucine (min) Arg1 1.0 0.3 1.1 p-S6K * – + Amino acid starvation *** *** *** 70 kDa f NS IL-4, days g Amino acids p-S6K S6K 10 15... COMMUNICATIONS | DOI: 10.1038/ncomms14711 a b Amino acids: – – + + + + + + + – + – ΦWT ΦKO ΦWT ΦKO ΦWT BMDMs: ΦKO p-S6K S6K d e 0.5 0.5 ** – + Amino acid starvation 0.5 A.U **1.0 ** ** 0.5 0.5... 10 30 10 30 IL-4, days l m + 400 – p-S6K 70 kDa S6K 70 kDa A.U Glucose (mg dl–1): 400 50 10 Amino acids: + + + 1.0 *** *** *** 1.0 0.5 0.5 *** *** *** 0 ConA (ng/ml) j RELMα ** * ** 0 IL-6 iNOS