ac 2017 00046k 1 2 Correction to Optimized Fragmentation Regime for Diazirine Photo Cross Linked Peptides Sven H Giese, Adam Belsom, and Juri Rappsilber* Anal Chem 2016, 88, 8239−8247 DOI 10 1021/acs[.]
This is an open access article published under a Creative Commons Attribution (CC-BY) License, which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited Addition/Correction pubs.acs.org/ac Correction to Optimized Fragmentation Regime for Diazirine Photo-Cross-Linked Peptides Sven H Giese, Adam Belsom, and Juri Rappsilber* Anal Chem 2016, 88, 8239−8247 DOI: 10.1021/acs.analchem.6b02082 n our paper entitled “Optimized Fragmentation Regime for Diazirine Photo-Cross-Linked Peptides”, there are two separate textual errors, one in Figure 6B (and repeated in the TOC graphic) and one in the Data Analysis section I Results: Decision tree representation (Figure 6B and TOC graphic) In Figure 6B and again in the TOC graphic, a visual decision tree was created from the m/z binning in Figure 4D (compare also Supporting Information Figure S2) Unfortunately, an algorithmic error introduced an offset of 200 m/z in the representation of the decision tree The decision tree is corrected here (Figure 6B) Data Analysis The used modification masses were partly not correctly stated Instead, the following modification masses are the correct ones: “SDA” cross-link, 82.0418 Da (C5H6O); “SDA-loop”, 82.0418 Da (C5H6O); “SDA-hydro”, 100.0524 Da (C5H8O2) The reaction scheme in Figure S9 shows the derivation of these masses During our database search, however, we used the correct masses for the cross-linker reaction between two peptides (“SDA”, 82.0418 Da) as well as for the “SDA-loop” (82.0418 Da) The “SDA-hyd” modification mass was not used in the database search Figure 6B Decision tree with corrected m/z boundaries Figure S9 Cross-link reactions of sulfo-SDA © 2016 American Chemical Society A DOI: 10.1021/acs.analchem.7b00046 Anal Chem XXXX, XXX, XXX−XXX Analytical Chemistry Addition/Correction The original text at the bottom page of 8240: variable modifications, carbamidomethylation on cysteine; oxidation on methionine; cross-linker, SDA (mass modification: 109.0396 Da) In addition, variable modifications by the hydrolyzed cross-linker (“SDA-hyd”, mass modification: 82.0413 Da) and loop-links (“SDA-loop”, mass modification: 83.0491 Da) were allowed The correct text should be as follows: variable modifications, carbamidomethylation on cysteine; oxidation on methionine; cross-linker, SDA (mass modification: 82.0418 Da) and loop-links (“SDA-loop”, mass modification: 82.0418 Da) were allowed B DOI: 10.1021/acs.analchem.7b00046 Anal Chem XXXX, XXX, XXX−XXX ... methionine; cross-linker, SDA (mass modification: 82. 0 418 Da) and loop-links (“SDA-loop”, mass modification: 82. 0 418 Da) were allowed B DOI: 10 .10 21 / acs.analchem.7b00046 Anal Chem XXXX, XXX, XXX−XXX... modifications by the hydrolyzed cross-linker (“SDA-hyd”, mass modification: 82. 0 413 Da) and loop-links (“SDA-loop”, mass modification: 83.04 91 Da) were allowed The correct text should be as follows: variable... original text at the bottom page of 824 0: variable modifications, carbamidomethylation on cysteine; oxidation on methionine; cross-linker, SDA (mass modification: 10 9.0396 Da) In addition, variable