1296 Imaging of Lentivirus Mediated Reporter Gene Expression in Living Mice Molecular Therapy Vol 5, No 5, May 2002, Part 2 of 2 Parts Copyright © The American Society of Gene Therapy S423 ����������![.]
!!"7 " "!"! 1294 A Novel Human Derived Reporter Gene for NonInvasive Imaging in Humans: Mitochondrial Thymidine Kinase T ype Type Vladimir Ponomarev,1 Mikhail Doubrovin,1 Inna Serganova,1 Lyudmila Ageeva,1 Tatiana Berestan,1 Suren Soghomonian,1 Alexander Shavrin,1 Julius Balatoni,2 Ronald Finn,2 Ronald G Blasberg,1 Juri Gelovani Tjuvajev Neurology, MSKCC, New York, NY, United States; Radiochemistry/Cyclotron Core Facility, MSKCC, New York, NY, United States 56 + " " 1 -!> / ) C , - A,/ $*' *" - ?!/ $*' A, ?! $*' - / ##D." 4:; ' 3 4:;% A, ?! ?'( A, ?! G 86"?'4 =C" @ =C" 86" @ " -' "/ 4:;" A, ?! " 4:; ($ G -L9& / 8,6"?'4 -8&9 %/ E !> , ,6 ,6 8,6"?'4 E )*) 4:;% A, ?! G ?'( -6B9 4/ A 86"?'4 99B:±99,9 999;±999,%% 4:;% A, ?! 4:; - %" 89/ A =C" 9998±9998 9998±9998 %% 4:;% A, ?! 4:; - % " 8/ $&9 ' " 99= 899 4:;% A, ?! " 4:; !> G 4:;% A, ?! " ,6 E 9,6±995 99=:±999,H % 8,6"?'44:;% A, ?! 4:; 4:;% A, ?! % % :;±8; ,;±88 I 4:; % % 86±, 6±9= (*)+( A, !> E 8,6"?'4 A, " " E 8,6" ?'4 !> A, 1295 Murine Dendritic Cells Are T ransduced More Transduced Efficiently by rAA V Serotype V ectors Compared with rAAV Vectors Other Serotypes Karl L Womer,1 Sihong Song,2 Scott A Loiler,3 Chengwin Li,4 Mark A Atkinson,5 Terence R Flotte,3 Michael Clare-Salzler.5 Renal Division, Department of Medicine, The University of Florida, Gainesville, FL, United States; 2Department of Pharmaceutics, The University of Florida, Gainesville, FL, United States; 3Department of Pediatrics, The University of Florida, Gainesville, FL, United States; 4Gene Therapy Center, The University of North Carolina, Chapel Hill, NC, United States; Department of Pathology, Immunology, and Laboratory Medicine, The University of Florida, Gainesville, FL, United States ( +( $ -$ / " $ @ ''. - ''./ 1 3 Molecular Therapy Vol 5, No 5, May 2002, Part of Parts Copyright © The American Society of Gene Therapy ''. 8"& "8" - '' / " ) ''. 3 '' # " -2#/ 6 #"(? D"6 89= $ ''. '' * ( & !?4 8, ''. (3 '' 3 >D(' )*) ' ? 8 ''. 8 ,"& 3 '' $ -O99998'*).'/ 3 3 8, - / '' 3 ''. 8-8B9%D/,-89;=%D/ =-,85%D/ 6 & (*)+() $ ''. 8 ? ' ''. $ $ 1296 Imaging of Lentivirus Mediated Reporter Gene Expression in Living Mice Abhijit De,1 Xiaoman Zhou Lewis,1 Sanjiv Sam Gambhir.1 Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, UCLA School of Medicine, Los Angeles, California, United States )E 7 C." 8 3 # 7 ' C(."8 7H0 - / - / ->#./ -@>(/ -( / -( " 7H0"" / -*,'/ 3 -?'( / $"D 6&9 %89 *,' 3 -&389B/ E 6 G &"B ,& % % - ./ - G -?D4/ = $"D % E F ".( -$/ 3 % %,% - / !> C(.8" 7H0 3 G ? "8: S423 !!"7 " "!"! -?C2 / C(.8" =5 A G ? " 8: 3 -?$ / 3 H$% @ 7 - -( " 7H0"" / *,' 3 ?'( ,&H ?D4 3 8 3 - G :"89 / :8,389 ;±8;9, -!O99&/ 8;8389B±&B ( !> ?C2 H$% 9B&:±99,& - / 98,5±99,= - / ?$ !> -6&8±9,&/ -6B&±9,,/ @ $ ?D4 89B389;±8=,B 88 $ . " ?D4 . C(.8" 7H0 ? !> ?$ . 3 C(.8" 7H0 7 G -C(.8" 7H0 / 3 !> $ > 3 2" > #'@ ? 3 > > #'@ ,"= ?! -#?/ 2" -*"B@>C @' #'@"! A 3 6 " ' 8&H $*' & ! A 3 -#? &8";8/ ,",6 > "! A ," ?! 3 2" - / 8 = & B > #'@"! A ="89 3 2" -#? =&&"B9B/ " -#? 6;";6/ ? ?! 2" 3 3 > > #'@ 3 2" > 2" " 3 1298 c-99m-Labeled Ad Vivo Tc-99m-Labeled In V ivo Imaging Detects T Redirected to Lung Following Intravenous Dosing 1297 Regulated T ransgene Expression in BTransgene Lymphocytes from Lentiviral V ectors Carrying the Vectors Immunoglobulin Heavy Chain Enhancer with Matrix Attachment Regions Carolyn Lutzko,1 Dinithi Senadheera,1 Diane Skelton,1 Denise Petersen,1 Donald B Kohn.1 Research Immunology/Bone Marrow Transplantation, Childrens Hospital Los Angeles, Los Angeles, CA, United States D -C( / ( 1 3 C( 1 3 2" C # 3 2" 3 ! A G (* " 2" 3 -> / 3 -> #'@/ ! A #. - 3 ?!/ S424 K R Zinn,1 T R Chaudhuri,1 V N Krasnykh,2 D T Curiel,2 P N Reynolds.2 Department of Radiology, University of Alabama at Birmingham, Birmingham, AL, United States; 2Department of Medicine, Pathology, and Surgery, Division of Human Gene Therapy; and the Gene Therapy Center, University of Alabama at Birmingham, Birmingham, AL, United States ( +(9 E -' / ' # ' 0 " ' 3 ? ' E 3 "55 ' B"C 3 ' B"C E "55" ' E )9 ' "55-/ 0 -* 2 8;7 :5;"5988555/ ' "8 ? 6 "55" ' =9 525 E ' " G -'>/ - @ *2 857:=:" :6, ,998/ "55" ' -8=38988 % / Molecular Therapy Vol 5, No 5, May 2002, Part of Parts Copyright © The American Society of Gene Therapy