1239 The Selective Amplifier Gene Promotes Multiclonal Expansion of Hematopoietic Stem/Progenitor Cells in a Nonhuman Primate Molecular Therapy Vol 5, No 5, May 2002, Part 2 of 2 Parts Copyright © The[.]
" # " C."8" #*$ -89;"89: % / 3 -> ?!/ 3 &9 - 8B&±89 / B B 2 $, $6 $: $85 = -; " / !@ > ?! !@ 8 > ?! 89& - 8 899999 / ' ' $*'" > ?! ' $, $6 $: $85 C 3 .(." C."8" ( 3 !"" # " 1238 Optimising Retroviral-Mediated Gene T ransfer Transfer into Hematopoietic Stem Cells under Clinical Relevant Conditions Sonja Naundorf,1 Stephanie Laufs,2 Axel A Fauser,1 Hans-Georg Eckert,1 W Jens Zeller,2 Stefan Fruehauf,3 Klaus Kuehlcke.1 European Institute for Research and Development of Transplantation Strategies (EUFETS AG), Idar-Oberstein, Germany; 2German Cancer Research Center (DKFZ), Heidelberg, Germany; 3Department of Internal Medicine V, University of Heidelberg, Heidelberg, Germany @ -( G ,999/ $=6J @ ®-@*/ - / " -A ,99,/ @* ' G D= DB ' 8 -#$@8/ $*' *)$%($ @ 8,= 3 1 !@ ) @* 89 % " 3 " ' *)$%($ = % " 3 " *)$% ($ ? " @* % " 3 " ( " @* D= DB Molecular Therapy Vol 5, No 5, May 2002, Part of Parts Copyright © The American Society of Gene Therapy ( S !@ 3 D= DB ) " ' 3 1239 The Selective Amplifier Gene Promotes Multiclonal Expansion of Hematopoietic Stem/Progenitor Cells in a Nonhuman Primate Masaaki Takatoku,1 Yutaka Hanazono,2 Takeyuki Nagashima,3 Hiroaki Shibata,4 Naohide Ageyama,4 Yasuji Ueda,3 Akihiro Kume,2 Cynthia E Dunbar,5 Keiji Terao,4 Mamoru Hasegawa,3 Keiya Ozawa.1,2 Division of Hematology, Department of Medicine, Jichi Medical School; 2Division of Genetic Therapeutics, Center of Molecular Medicine, Jichi Medical School; 3DNAVEC Res Inc.; 4Tsukuba Primate Center, National Institute of Infectious Disease; Hematology Branch, NHLBI, NIH 3 " -C(/ C( -(' / "(? - 3 / " (' 3 3 -A 2 855;I597=::6# A# 8555IB789=:F @ !)@/ @ -#/ " >!)@ 3 >!) ? >!)" (' >!)@ @ # - >!)@" @ >!)@"#/0 " >!)@->!)@/>!)@" @ >!)@"# D=" 2%?= >!) * >!)" (' 2%?= " (' >!)%>!)@ (' % $=6J >!)@ >!)@" @ >!)@"# >!)@" # >!) =9 >!)@"# 3 "AJ >!)@"#" " ?4 >!) >!)@"# 3 2 &" " #(. 3 >!)@"#" "+?! - / +?! >!) +?!" +?!J - 3 89H/ >!) ) >!)@"#" "+?!" +?!J - 895±B9 H ,8B±8,; H/ 3 >!) >!) >!)@"# >!)" S404 1241 Selective Expansion of Retrovirally T ransduced Transduced Hematopoietic Cells Stefan Stein,1 Anja Luedemann,1 Manuel Grez.1 Molecular Virology - Gene Therapy Program, Georg-SpeyerHaus, Frankfurt, Germany -!C( / 1 $ - $/ - $ !C( C @ " F" $ -99B"9,H/ 1 -8;"66 89B % / F"($ " ( $ ? 3 " 3 C 3 1 ' -"/ C( 3 " G - ?A&9B I '@'$ ! / G -'!,98:;/ ( ' D"= G '!,98:;- / 3 86 @ $=6J " ? 3 (H'$*'2 ( H' >3 3 3 1242 Decreased Homing of T ransduced Human Bone Transduced Marrow CD34+ Cells in the NOD/SCID Mouse Model Kristin M Hall,1 Kenneth Cornetta,1,2,3 Rafat Abonour,2 Edward F Srour.1,2,4 Microbiology/Immunology; 2Medicine; 3Medical and Molecular Genetics; 4Pediatrics, Indiana University School of Medicine, Indianapolis, Indiana, United States * " $=6J 4G -2#/ $=6J *)$%($ G $=6J !AC,B $=6J *)$%($ 2# $=6J # $?(? ?D= 8 , Molecular Therapy Vol 5, No 5, May 2002, Part of Parts Copyright © The American Society of Gene Therapy ... 1238 Optimising Retroviral-Mediated Gene T ransfer Transfer into Hematopoietic Stem Cells under Clinical Relevant Conditions Sonja Naundorf,1 Stephanie Laufs,2 Axel A Fauser,1 Hans-Georg Eckert,1... Zeller,2 Stefan Fruehauf,3 Klaus Kuehlcke.1 European Institute for Research and Development of Transplantation Strategies (EUFETS AG), Idar-Oberstein, Germany; 2German Cancer Research Center (DKFZ),... 2German Cancer Research Center (DKFZ), Heidelberg, Germany; 3Department of Internal Medicine V, University of Heidelberg, Heidelberg, Germany @