221 Sustained High Levels of Gene Expression Achieved In Vivo by Electroporation of DNA Containing Microparticles Molecular Therapy Vol 5, No 5, May 2002, Part 2 of 2 Parts Copyright © The American So[.]
!"" # !"" # -(>'!/ E > -,99 % : / E $*'" G # " G (>'! 3 (>'! % * (>'! 3 89 ,9 218 Biodistribution and In V ivo Gene T ransfer of Vivo Transfer Liposomes Generated from Poly(Cationic Lipid) and DOT AP DOTAP Li Liu,1 Mark A Zern,1 Michael H Nantz,2 Jian Wu.1 Transplant Research Institute, University of California, Davis Medical Center, Sacramento, CA, United States; 2Department of Chemistry, University of California-Davis, Davis, CA, United States &'( 7 8," - 3/" ="- / -$) '!/ - / -$) '!" / - / -!D/ 3 " -2E ,998I 8,7,&8",&;/ !D" $) '!" $) '!"$)!> )7 7 !D" $) '!" $) '!" $)!> =78 Q=CR" =9 E 3 $*' E - =/ E D ,6 )*)7 =9 E Q=CR" " !D" E $) '!" $) '!"$)!>-=&B±6; :589±&9BI;=69±6,8O998M =/ !D" $) '!" $) '!"$)!> E $) '!" $) '!"$)!> ,," ,=" !D" "E D !D" "E E $) '!" $) '!"$)!> -=B,&±B;& 8B6&±68:I 885=±,8& % 899 O99&/ D -,& =;" / !D" "$*' E = E $) '!" "$*' $) '!"$)!>"$*'-65,58=±=&=B6 85B=,8±::9& 8=,6;,±66:&6 @D4% O998 M =/ (*)+(7 ) G " $) '!" $) '!"$)!># !D" $) '!" $) '!"$)!> = E !D" $) '!" $) '!"$)!> 219 Increasing the Therapeutic Index of Cationic Lipid:pDNA Complexes Using Dexamethasone Jennifer D Tousignant,1 Simon J Eastman,1 Steven Boule,1 Macy Joseph,1 Rebecca Rubenstein,1 Stephen A Rydberg,1 Qiuming Chu,1 Ronald K Scheule.1 Genzyme Corporation, Framingham, MA, United States ( 7 $*'-$*'/ 3 " 3 G Molecular Therapy Vol 5, No 5, May 2002, Part of Parts Copyright © The American Society of Gene Therapy 3 " 3 1 N2 ( 3 7$*' 3 ? " 1 3 3 7$*' ) 3 3 3 $ 3 " $ 3 3 &"89 G 3 #. 41 2 3 " ' 3 3 1 3 ? 7$*' " E 3 3 ? ′ 220 Development of a Dual-Luciferase Fusion Gene as a Sensitive Marker for Site-Directed DNA Repair Strategies Michael L Bennett,1 Jerome Schaack.1 Microbiology, University of Colorado Health Sciences Center, Denver, Colorado, United States @ 1 " $*' 69H 4 G 1 C G " G 3 3 > " $*' 1 " 1 &9F89"B-O8 ,99999/ 221 Sustained High Levels of Gene Expression Achieved In-Vivo by Electroporation of DNA-Containing Microparticles D Wang, K Roy, M L Hedley, S P Barman Zycos Inc, Lexington, MA, United States G $*' G S73 !"" # 1 G - 3/ ) G C 3 E $*' - "" / $*' $*'" - "" / G "" 1 # $*' &B % G O 89 -L5&H/# L85H $*' E # -&;2D%B 6"B / E - / &9 $*' -M:% / E 2 (>'! G - ( / " (>'!% 0 (>'! L899 # (>'! = "E ' 3 (>'! 59 3 86 E % $*' 222 Probing the Stoichiometry and Structure of a Lipid/Peptide/DNA T ransfection V ector Using Transfection Vector Fluorescence T echniques Techniques Alethea B Tabor,1 Erwann Guenin,2 Alastair Nicol,3 Michael Pilkington-Miksa,1 Susie E Barker,4 Paul Bell,1 Christopher A Hurley,1 Daniel Zicha,3 Helen C Hailes,1 Stephen L Hart.4 Department of Chemistry, University College London, London, United Kingdom; 2Laboratoire de Chimie Organique des Substances Naturelles, Centre de Neurochemie, Strasbourg, France; 3Imperial Cancer Research Fund, London, United Kingdom; 4Institute of Child Health, University College London, London, United Kingdom ' " " % %$*' D$ D -D7 $) #'7$)!>878 / -7 D 8B $*' / $*' -$/ 3 $ * -/ D$ 1 -?(/ ? ?( 3 D$ 3 $) #' $)!> 3 3 3 3 3 3 3 G 3 3 S74 223 DSPE-PEG Reduced Serum Complement Opsonization of Lipid-Protamine-DNA (LPD) Lipopolyplexes Patrick L Ahl,1 Pierrot Harvie,2 Edward Choice,2 Pervin Anklesaria,2 Ralph W Paul,2 Sally Cudmore,3 Daniel O′Mahony,3 Paul Meers.1 Drug Delivery, Elan Pharmaceuticals, Princeton, NJ, United States; 2Targeted Genetics Corporation, Seattle, WA, United States; 3Drug Delivery, Elan Pharmaceuticals, Dublin, Ireland D G " -' -855;/ 2 2 ' 8=,57 =;9"=:,/ ( " " 3 G " "$*' -D!$/ D!$ -D -855:/ &7 5=9"5=;/ -C -,998/# =7(88/D!$ 8, -$) '!7 878/8μ 8 μ $*' G " ? 1 &9H C&9 ' C&9 D!$ D!$ G $*'" D!$ -95 # / $) '!7 -87 8 = # / C&9 59H G D!$ D!$ D 89H D!$ 8& "E -!> / D!$ G C&9 =;H D!$ 89 $(!>"!> &A -!> #0 & A / G !> " D!$ D!$ 89 $(!>"!> &A 3 B9H 8& "E G ( G C D!$ G $(!>"!> D!$ !> G $(!>"!> $(!>"!> ,A-,A !> / C&9 58H =5H , ,9H $(!>"!> ;&9-;&9 !> / :;H C&9 ,9 3 G " > ( 224 Efficient Plasmid DNA Encapsulation into Medium Sized N-Acyl Phosphatidylethanolamine-Containing Liposomes for Gene Delivery Tong Shangguan,1 Xingong Li,1 Alla Polozova,1 Walter Perkins,1 Paul Meers.1 Elan Corporation, Princeton, NJ, United States $*' G ' " - / ' $*'" - / ( 1 G 699 ' $*' =9H $*' - / $* $*'"" , % $ 89H Molecular Therapy Vol 5, No 5, May 2002, Part of Parts Copyright © The American Society of Gene Therapy ... Biodistribution and In V ivo Gene T ransfer of Vivo Transfer Liposomes Generated from Poly(Cationic Lipid) and DOT AP DOTAP Li Liu,1 Mark A Zern,1 Michael H Nantz,2 Jian Wu.1 Transplant Research Institute,... Wu.1 Transplant Research Institute, University of California, Davis Medical Center, Sacramento, CA, United States; 2Department of Chemistry, University of California-Davis, Davis, CA, United States