212 of states and fates: predicting t cell immunity by the numbers

212 Of States and Fates Predicting T Cell Immunity By the Numbers Molecular Therapy Volume 23, Supplement 1, May 2015 Copyright © The American Society of Gene & Cell Therapy S83 CanCer – Immunotherapy[.]

Cancer – Immunotherapy, Cancer Vaccines I U266 myeloma cell line (NY-ESO-1+HLA-A2+), all NY-ESO-1 redirected T cells showed a strikingly high killing activity However, when we assess the alloreactive potential of the different redirected T cells in mixed lymphocyte reactions, we observed that the allogeneic lysis by SE T cells was markedly lower (p=0.05) than that of conventional TCR transfer cells (TR) These results were validated in NSG mice, where the genetically modified T cells were infused after the engraftment of the U266 myeloma All animals treated with tumor specific T cells were completely myeloma-free at the time of sacrifice, demonstrating the powerful anti-tumor potential of the NY-ESO-1 redirected T cells However, the overall survival of mice treated with TR vs SE cells was 26% vs 100% respectively (p