β2 adrenoreceptor Inverse Agonist Down regulates Muscarine Cholinergic Subtype 3 Receptor and Its Downstream Signal Pathways in Airway Smooth Muscle Cells in vitro 1Scientific RepoRts | 7 39905 | DOI[.]
www.nature.com/scientificreports OPEN received: 04 July 2016 accepted: 29 November 2016 Published: 04 January 2017 β2-adrenoreceptor Inverse Agonist Down-regulates Muscarine Cholinergic Subtype-3 Receptor and Its Downstream Signal Pathways in Airway Smooth Muscle Cells in vitro Jian Luo1, Yuan-hua Liu2, Wei Luo3, Zhu Luo1 & Chun-tao Liu1 Mechanisms underlying β2-adrenoreceptor (β2AR) inverse agonist mediated bronchoprotectiveness remain unknown We incubated ICI118,551, formoterol, budesonide, and formoterol plus budesonide, as well as ICI118,551 or pindolol plus formoterol, ICI118,551 plus forskolin, SQ22,536 or H89 plus formoterol in ASMCs to detect expressions of M3R, PLCβ1 and IP3 The level of M3R in the presence of 10−5 mmol/L ICI118,551 were significantly decreased at 12 h, 24 h and 48 h (P