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THÔNG TIN TÀI LIỆU
Cấu trúc
Abstract
Background
Methods
Results
Conclusions
Background
Results and discussion
Inhibition of Nxf1-mediated nuclear export via expression of dominant negative Nxf1 results in decreased virus production and cytoplasmic reduction of select influenza mRNAs
Immuno-purification of Nxf1 in A549 cells reveals NP, HA, and NA influenza viral mRNAs associate with Nxf1, while PA, PB1, and PB2 mRNAs do not
Influenza NS1 protein also associates with Nxf1/RNA immuno purified complexes
Leptomycin B treatment inhibits virion production but not influenza PA, PB1, or PB2 mRNA nuclear export in A549 cells.
Conclusion
Methods
Cells, virus, plasmids, LMB, and antibodies
Transfection
LMB treatment
Influenza A infection
Plaque and HA assays
Cellular fractionation and isolation of protein and RNA
Total extract preparation for immunopurification and associated RNA isolation