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[...]... preferred Plain glass Plasma possible Heparin Glutathione peroxidase Whole blood Heparin LDH Serum Plain glass Serum preferred Plain glass Plasma electrolytes possible Heparin Urea Serum (preferred) or plasma Plain glass or heparin Creatinine Serum (preferred) or plasma Plain glass or heparin Total protein Serum Plain glass Albumin (and globulin) Serum Plain glass Protein electrophoresis Serum Plain glass... quantities indicate the need for further investigations, including bladder and kidney palpation, ultrasound scan and cystoscopic examinations (see also Ch 4: ‘Urinary diseases’) Interpretation of parasitological test results Faecal worm egg counts These remain the basis of equine intestinal parasite surveillance and of monitoring the efficacy of control 23 Diagnostictechniquesinequine medicine programmes,... beta-1 globulin levels The sensitivity and specificity of this test for the presence of strongyles are low Liver failure associated with increases in beta-2 and gamma globulins Albumin : globulin ratios In health, the albumin : globulin (A/G) ratio approximates to 1.0 or more Shifts in the ratio may occur in a number of pathological states, but the informa15 Diagnostictechniquesinequine medicine Table... Rectal examination 57 Stomach intubation 57 Diagnostictechniquesinequine medicine Abdominal paracentesis 58 Laboratory aids 58 Indications for surgical exploration (laparotomy) 58 A strategic approach to the colic case 58 III Clinical pathology 60 Serum biochemistry 60 Total protein 60 Albumin 61 Globulins 61 Albumin : globulin ratios 61 Serum protein electrophoresis 61 Serum alkaline phosphatase ... may be noted in a variety of equine diseases including haemolysis, liver disease, impaction colic and any condition associated with a reduction in food intake However, it is unusual for serum bilirubin to be elevated inequine liver disease; an increase may be diagnostically useful but normal values do not discount liver disease In fasting (or inappetence) there is a physiological decrease in the removal... anaemia in the horse in contrast to other species, since equine erythrocytes mature within the bone marrow rather than in the 12 circulation, even during intense erythropoiesis In consequence, the MCV is seen to progressively increase or decrease over time, but it usually remains within a ‘normal’ reference range That said, macrocytic anaemia is most commonly seen in haemorrhagic conditions, including intestinal... Plain glass or heparin Oestradiol Serum (preferred) or plasma Plain glass or heparin Oestrone sulphate Serum (preferred) or plasma Plain glass or heparin eCG Serum Plain glass Blood electrolytes Serum electrolytes Other biochemistry Blood hormones Continued 5 Diagnostictechniquesinequine medicine Table 1.2 Appropriate samples and containers for clinicopathological tests—cont’d Test Sample Container/medium... single system Serum Plain glass Urine analysis Urine Clean non-leak container Urinary fractional excretion of electrolytes Urine plus serum (preferred) or plasma Clean non-leak container plus plain glass or heparin Culture Midstream Sterile non-leak container Oestrogens (Cuboni test) Urine Clean non-leak container Cytology Fluid EDTA Biochemistry Fluid Plain glass Culture Fluid Plain sterile container... history and clinical examination, rather than ‘trawling’ a profile However, when the clinical examination is inconclusive, such profiles can be useful in identifying further routes of investigation A typical profile is shown in Table 1.5, together with a tentative interpretation of abnormalities and suggestions for further investigations Equine chorionic gonadotrophin (eCG) may be detected in mares where... check PCV and total serum protein Tissue catabolism: check indicators of inflammation High protein diet Renal failure: compare serum creatinine concentration Total protein High Dehydration: check PCV High globulin: check SPE: Raised alpha-2 globulin: acute inflammation Raised beta-1 globulin: parasitism Raised gamma globulin: chronic inflammation Albumin Low Protein-losing enteropathy If diarrhoeic: . (preferred) or plasma Plain glass or heparin
eCG Serum Plain glass
Continued
Diagnostic techniques in equine medicine
6
Test Sample Container/medium
Blood culture
Aerobic/anaerobic.
provide further information. However, laboratory
CHAPTER
Diagnostic techniques in equine medicine
2
screening may play a part in preventive medicine
and performance