ThemeGallery PowerTemplate FUNDAMENTAL GAS CHROMATOGRAPHY FUNDAMENTAL GAS CHROMATOGRAPHY 4 2 CONTENT QAC DEPARTMENT ORGANIZATION INTRODUCTION OF BSR LAB SAMPLING SYSTEMS IN BSR 4 1 2 3 1 What Gas Chro.
2 FUNDAMENTAL GAS CHROMATOGRAPHY CONTENT What Gas Chromatography is QAC DEPARTMENT ORGANIZATION Carrier and detector support gas samples OF BSR LAB 23 Injecting INTRODUCTION Separating components SAMPLING SYSTEMS IN BSR 35 Detecting components Interpreting Chromatogram What Gas Chromatography is 1.1 Brief story history of Chromatography: In the early 1900’s: M Tswett published his work on separation of plant pigments He coined the term chromatography (literally translated as color writing) and scientifically described the process – earning him the title “father of Chromoatography” W Ramsay published his worked on separation of mixture of gases and vapors on adsorbents like charcoal In the early 1940’s: GIÁ 2014 the VÀpossibilities ĐỊNH HƯỚNG KPI 2015 andĐÁNH A Martin R.Synge firstKPI suggested of gas chromatography in a paper published in Biochem J, v.35, 1358 (1941) Martin won a Nobel Prize for his work in Partition chromatography CÁC ĐỀ XUẤT-KIẾN NGHỊ In the early 1950’s: A Martin and A James published the epic paper describing the first gas chromatography What Gas Chromatography is 1.2 The Chromatography definition: - A physical method of separation in which the components to be separated are distributed between phases, one of which is stationary while the other moves in a definite direction (IUPAC definition) - The mobile phase: Liquid (methanol, water….): Changing dielectric strength Gases (helium, hydrogen…): Temperature - The stationary phase: ĐÁNHsilica, GIÁ KPI 2014 VÀ ĐỊNH HƯỚNG KPI 2015 Solids3(alumina, carbon…): Adsorption chromatography Liquid (siloxanes, polyethylene glycol…): Partition (distribution) chromatography CÁC ĐỀ XUẤT-KIẾN NGHỊ What Gas Chromatography is Adsorption: sorption on the surface of the stationary phase sorption ORGANIZATION Absorption: into the bulk of the stationary phase ĐÁNH GIÁ KPI 2014 VÀ ĐỊNH HƯỚNG KPI 2015 CÁC ĐỀ XUẤT-KIẾN NGHỊ What Gas Chromatography is 1.3 The Gas Chromatography ORGANIZATION What Gas Chromatography is 1.4 What is the deal with GC? ORGANIZATION What Gas Chromatography is 1.5 The characteristics for Gas Chromatography: What Gas Chromatography is 1.6 Sample Requirements for Gas Chromatography What Gas Chromatography is 1.7 The typical GC System Schematic Interpreting Chromatograms 6.1 Peak measurement Two basic measurements can be made on a peak: • The time after injection when the peak is detected • The size of the peak The retention time: the additional time caused by the component’s interaction with the stationary phase in the column The peak size: Size can be measured either as peak area or peak height, both measured relative to a constructed baseline Peak height This is the simplest measurement, requiring only a ruler It is the vertical distance from the top of the peak to the baseline Peak area This is the area enclosed by the peak signal and the baseline under it It is best measured by electronic means Interpreting Chromatograms Interpreting Chromatograms Interpreting Chromatograms Interpreting Chromatograms Interpreting Chromatograms Interpreting Chromatograms Interpreting Chromatograms Interpreting Chromatograms Interpreting Chromatograms Interpreting Chromatograms Interpreting Chromatograms Interpreting Chromatograms Interpreting Chromatograms ... What Gas Chromatography is 1.3 The Gas Chromatography ORGANIZATION What Gas Chromatography is 1.4 What is the deal with GC? ORGANIZATION What Gas Chromatography is 1.5 The characteristics for Gas. .. characteristics for Gas Chromatography: What Gas Chromatography is 1.6 Sample Requirements for Gas Chromatography What Gas Chromatography is 1.7 The typical GC System Schematic What Gas Chromatography. .. Partition chromatography CÁC ĐỀ XUẤT-KIẾN NGHỊ In the early 1950’s: A Martin and A James published the epic paper describing the first gas chromatography What Gas Chromatography is 1.2 The Chromatography