Aurora B kinase promotes CHIP-dependent degradation of HIF1α in prostate cancer cells Kuntal Biswas1, Sukumar Sarkar1, Neveen Said1,3, David L Brautigan2 and James M Larner1* Department of Radiation Oncology and 2Center for Cell Signaling, Department of Microbiology, Immunology & Cancer Biology University of Virginia School of Medicine, Charlottesville, VA 22908 present address: Department of Cancer Biology, Wake Forest School of Medicine, WinstonSalem, NC 27101 *Correspondence: James M Larner Address: University of Virginia, 1300 Jefferson Park avenue, West complex Rm No-7121, Charlottesville, Virginia 22908 Phone: 434-924-2393; Fax: 434-243-9789; Email: JML2P@virginia.edu Running Title: Aurora B kinase enhances CHIP mediated HIF1α degradation Key words: Hypoxia, prostate cancer, CHIP, Aurora B, HIF1α “The authors declare no conflicts of interest” Supplementary Figure 1: Depletion of CHIP enhances tumor growth in AR negative prostate cell A Growth of subcutaneous tumors of human PC-3 cell xenografts in SCID mice PC-3 cells were stably knocked down for CHIP by lentiviral transduction with short hairpin RNA targeting CHIP (shCHIP) and non-targeted RNA (shNT) used as controls Tumor growth was significantly increased with p