Images Hippocampal neuron imaged using the Applied Precision DeltaVision OMX system by Eric Dent, University of Wisconsin-Madison, USA Mouse intestine imaged using the Applied Precision DeltaVision OMX system by Paul Appleton, University of Dundee, UK Fixed HeLa cells imaged using the Applied Precision DV Elite system by Paul Andrews, Wellcome Trust Biocentre, University of Dundee, UK Chinese hamster ovary imaged using the Applied Precision DV Elite system by Paul Goodwin, Applied Precision Co-culture of human epidermal keratinocytes and melanocytes imaged using the GE Healthcare IN Cell Anayzer by Emeline Ratineau, BIOalternatives, France Neurons and astrocytes isolated from rat hippocampus imaged using the GE Healthcare IN Cell Anayzer by Miriam Ascagni, DIBIT San Raffaele Scientific Institute, Italy Zebrafish expressing nitroreductase in pancreatic islets imaged using the GE Healthcare IN Cell Anayzer by Steven Chen, UCSF / Small Molecule Discovery Center, USA