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ĐẠI HỌC QUỐC GIA HÀ NỘI VIETNAM NATIONAL UNIVERSITY, HANOI ISSN 0866 - 8612 @ JOURNAL KHOA HOC TU NHE NATURAL SCIENCE Volume 27, No 2S, 2011 : | 2S (2011) 226-238 Sciences and Technology 27, No VNU Journal of Science, Natural | | | and Taxonomic study of a thermophilic bacterium duction ion of culture conditions for protease pro optimizat Le Quyen!, Ha Thi Hang’, Dao Thi Luong’*, Vi Dai Lam’, Tran Thi Van Hop' Pham Duc Ngoc’, Duong | i, Vietnam Biotechnology, 144 Xuan Thuy, Hano 1YNU Institute of Microbiology and i, Vietnam ty of Science, 334 Nguyen Trai, Hano Faculty of Biology, VNU Universi Received 09 September 2011 | ear metres ceees eae was isolated from uced high level of extracellular protease Abstract A bacterial strain YS24 prod observation, cell ny colo Based on the - Tuyen Quang soil sample collected at Yen Son was identified as YS24 n strai nucleotide sequence, the growth were morphology and the analysis of 16S rDNA rial bacte and on s of extracellular protease producti K,HPO,-5; Bacillus subtilis The highest level ; ;O-5 4.7H MgSO ose-5; peptone-7.5; Gluc (g/l): V um medi using by obtained hed a maximal esponded with bacterial growth and reac corr on ucti prod yme Enz ,0-0 FeSO,.7H shaker under following erium was cultured on the orbital level of 1,390 U/ml when the bact 5°C, medium pH of 7.0 ulum size of 7% , temperature of 40-4 condition: at speed of 200 rpm, inoc ose or galactose The The best carbon source was either gluc 8.0 and incubation time in 48 hours highest level at pH the me at extract Protease activity reached best nitrogen source was peptone or l activity after initia its enzyme retained 93, 86 and 76% of lost completely of 8.0 and temperature of 50 °C The was activity 80 °C for 10 min, respectively Enzyme BAR treatment at 60 °C, 70 °C and 90°C after exposing to temperature above properties taxonomic, culture conditions, enzyme ase, Keywords: Bacillus subtilis, prote the genus Many bacteria belonging to enzymes Bacillus excrete large amounts of the Introduction into enzymes Proteases are the most important industries accounting for about 60% of the total diverse have They market of enzyme industries, applications in a wide variety of medium [2] Microbial only property of all living organisms, ial tant microorganisms that produce a subs x-ray films and for recovery of silver from used amount of extracellular protease exploited commercially [3] [1] E-mail: luongdt@vnu.edu.vn culture enzyme proteases dominate the world wide d share of market, eg accounting for a two - thir the detergent industry [3] inherent Although protease production is an tanning and such as in detergent, food, leather als, garments processing, fiber, pharmaceutic 47695 * Corresponding author Tel.: 84-4-375 the 226 have been ... Sciences and Technology 27, No VNU Journal of Science, Natural | | | and Taxonomic study of a thermophilic bacterium duction ion of culture conditions for protease pro optimizat Le Quyen!, Ha Thi Hang’,... industries, applications in a wide variety of medium [2] Microbial only property of all living organisms, ial tant microorganisms that produce a subs x-ray films and for recovery of silver from used amount... Nguyen Trai, Hano Faculty of Biology, VNU Universi Received 09 September 2011 | ear metres ceees eae was isolated from uced high level of extracellular protease Abstract A bacterial strain YS24
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