D.T Luong et al / VNU Journal oƒScience, Natural Sciences and Technology 23, No 1S(2007) 38 -45
Bullera haininhensis sp nov., a new ballistoconidium-forming yeast species from Cuc Phuong National Park
Dao Thi Luong'”, Masako Takashima’, Pham Van Ty” Nguyen Lan Dung i
Takashi Nakase*
‘Institute of Microbiology and Biotechnology, Vietnam National University, Hanoi ?Microbe Division / Japan Collection of Microorganisms, RIKEN BioResource Center, Japan
7Faculty of Biology, Hanoi University of Science, VNU -Hanoi
‘Biological Resource Center (NBRC), Department of Biotechnology, NITE, Japan
Received 15 August 2007
Abstract Three ballistoconidium-forming yeast strains, VY-60; VY-86 and VY-140, isolated from the wilting leaves in Cuc Phuong National Park in Vietnam, were assigned to the genus Bu/lera Derx based on morphological and chemotaxonomical characteristics Based on the partial sequence analyses of of 18S rDNA, internal transcribed spacer regions (ITS) and D1/D2 region of 26S rDNA, they were found to represent a new species Bullera haininhensis is proposed for this species and VY-86" is chosen as the type strain of the species
Key words: Bullera haininhensis sp nov; systematic
1 Introduction
In 1999, 121 strains of ballistoconidiogenous yeasts were isolated from plant materials collected the Cuc
Phuong National Park, Ninh Binh Province, Vietnam
Eighty-five strains wereselected by their morphology of ballistoconidia and colonies and carried out details taxonomic studies Among these strains, four new species of the genus Kockovaella and two new species of the genus Bullera have already been described as K calophylli, K cucphuongensis, K litseae and K vietnamensi[]]; Bullera ninhbinhensis [2] and B hoabinhensis [3] In this paper, the taxonomic positions of three undescribed yeast strains of the genus Bullera are discussed and they were identified as Bullera haininhensis sp nov
2 Materials and methods
Yeast strains Yeast strains used in this study were isolated from plant leaves collected in Cuc Phuong National Park of Ninh Binh Province, Vietnam (Table 1), using the ballistoconidium -fall method on YM agar as reported by Nakase and Takashima [4]
Morphological, physiological and biochemical characteristics Most methods used for the analysis of morphological, physiological and biochemical characteristics followed the procedures described by Yarrow [5] The determination of maximum growth temperature was carried out in YM broth, using metal block baths The assimilation of nitrogen compounds was determined by the method of Nakase and Suzuki [6] The vitamin requirement test followed the method of Komagata and Nakase [7]
Chemotaxonomic characteristics
Extraction, purification and identification of
ubiquinones were carried out according to the method of Nakase and Suzuki [8] The presence of xylose in the cells was analyzed by thin-layer chromatography after hydrolyzing the cells with trifluoroacetic acid [9]