TC khoa hoc VNU vol 31 no 2s

, T\ll)chi KhoahQCDHQGHN:KhoahQCTVnhienva Cong ngh~,T~p31, sA 2S (2015)274-280 ? , Anh huang cua kim loai nang (Zn, Pb) den cay keo neo (Limnocharis Flava) a giai doan cay non Tr§n Thi Tuyet Thu1,*, Nguyen Vi~t Hi~p2, Duong Thanh Van1 I Khoa Moi trutmg,.Truong Dai hoc Khoa hoc T1/nhien, BHQGHN, 334 Nguyin Triii;Ha N{Ji,Vi~tNam 2Vi~nTh6 nhuimg Nang hoa, Bong Ngac, Tit Liem; Ha Nvi, V~ Nam Nh~ 15 thang nam 2015 Chinh sua 16 thang nam 2015; Chip nh~ dang ngly 21",1 .s Tom tit: Cay keo neo (LimnocharisFlava) 130thuc v~t sieu tfch liiy kim loai ~ng Cay thi nghiem (5 tu~n tu6i, trung blnh 4-5 la, chieu cao 10,93 em) duoc tr6ng dit gay nhi~m Zn, Pb a ding ham hrong 80 va 240 ppm Ket qua sau 10,20,30,40 chua gay anh huang b~t 19i den chieu cao, di~p luc t6ng s6 rna kfch thfch cay phat trien Trong d~t b6 sung Zn, a thoi diem 40 ngay, chien cao cay cao nh~t a en.2 (240 ppm) 25,98 cm va th~p nh~t a CfO (d6i chimg) 23,92 em; ham luong di~p luc t6ng s6 Ian nh~t a Cf1.2 0,043 rag/em' va th~p nh~t a CfO 0,039 mg/cnr' Nguoc lai, a d~t b6 sung Pb dli gay nhieu b~t 19i, cu the la chieu cao va diep luc t6ng s6 giam manh sau 30 - 40 Trong d6, thoi diem 40 ngay, chieu cao cay cao nh~t aCTO (d6i chimg) 23,92 em va th~p nh~t a Cf2.2 (240 ppm) 18,99 em, ham luong di~p luc t6ng s61an nh~t a CfO 0,039 mg/cm2 va th~p nh~t a Cf2.2 0,Q3 mg/cm", Su tfch liiy Zn, Pb sinh kh6i tY l~ thuan voi hrong b6 sung va ph~n r~ nhieu han than laoThoi diem 40 ngay, t6ng luong tfch liiy Zn cao nhat U CT1.2 415,7 ppm va th~p nh~t U CTO 145,46 ppm, Pb cao nh~t a Cf2.2 8,27 ppm va th~p nh~t aCTO 2,66 ppm Tit khoa: Kim loai n~ng, kern, chi, cay keo neo, Lirnnocharis Flava I I I ' i D,t vin d~ Cay keo neo (Limnocharis Flava) co nguon g6c a vung nhiet dOi va c~n nhiet dOi CMu My Vi~t Nam cay moe hoang dai ho~c chu y€u tai vimg d~t ng~p mroc khu VI,lC d6ng b~ng song Ciru Long Phan than hi non thuCmgdUQ"c su d\mg U~mrau an va la m6n rau d~c SilO khong th~ thi€u m6n Iftuca keo cua nguOi dan NamBQ o • Tac gia lien h~.DT:84-912733285 Email: tranthituyetthu@hus.edu.vn 274 Hien tren th€ gioi d1lco mQt s6 nghien ciru v~ anh huang cua kim loai nang (KLN) d€n cay keo neo Theo Chadran va nnk (2009) [1], thuc v~t phat trien tren d~t nhi€m KLN thl ham hrong di~p luc giam tang tang ch~t r~n hoa tan, clorua, sunphat hoac nitrat Ben canh d6, di~p l\lc t6 bi giam cling c6 th~ la k€t qua cua sv phan huy mang l\lc ll;lPvOi kim lo~i du thua lam giam kha nang quang hqp cua laoTheo Kamarudzarnan va nnk (2005) [2], tr6ng cay keo neo d~ xu ly nu&c thai chua Fe va Mn theo cong ngM dong chay ngang c6 th~ lo~ bo 91,5 - 99,2% va 94,7 - 99,8% Fe va Mn i T.T.T Thu va nnk: ITgp mroc ri rae chi Khoa h9CDHQGHN: Khoa h9C TITnhien a Malaysia Con theo Abhilash va nnk (2009) [3], cay keo neo An BQ e6 th€ loai 93 - 95% Cd dAt0 nhi~m 0,5 - ppm Cd Nghien elm cua Anning va nnk (2013) [4], sau 12 tuan tr6ng keo neo tren dAt ng~p nuoc nhiem KLN ciing da chi sir tfch liiy Fe, Cu, Zn, Pb, Hg tang d~n theo thoi gian, hieu qua tich lily 20 - 77% va t~p trung chu y~u r~ > than hi Trong d6, Fe tfch liiy nhieu nhAt phan r~ 456 - 1549 ppm va Pb 1,2-7,6 ppm B~n Vi~t Nam chua e6 cong b6 v€ anh huang cua KLN d~n cay keo neo, VI v~y, nghien ciru da dua co sa khoa h9C khuyen cao viec tr6ng keo neo tren dAt0 nhi~m KLN dS sir dung lam rau an, d6ng thoi c6 th€ sir dung cay keo neo xir 19 dAt0 nhiem a a Nqi dung, dai tll(}'Dg va phuong nghien cUu phap NQi dung nghien elm: (1) Anh huang cua Zn d~n cay keo neo, (2) Anh huang cua Pb d~n cay keo neo Cay keo neo duoc nhan gi6ng b~ng hinh thirc sinh san vo tfnh, dt cay moe tren cay m~ a thOi diSm tuftn tu6i Tr6ng cay vao m6i ch~u thf nghi~m (kich thuae 30 x 30 x 40 cm, chua kg dAtkho da d6 ng~p cm nuac cAtdS 6n dinh di€u ki~n moi trmmg va ph1,lch6i ho~t tinh sinh hQCdAt mQt tuftn) Sau tu~n tr6ng cay phat tri€n khoe m~nh, chi€u eao trung blnh la 10,93 cm, s6 la tuang d6ng (4 - 5) la, bQr~ bam dAt6n dinh thl b6 sung Zn, Pb BAt thf nghi~m la dftt phil sa song H6ng duQ'cb6i hang nam, thu~n IQ'icho S\l pMt tri€n cua cay keo neo BAt c6 phin ting gftn trung tinh (pH = 6,8), chftt hfru co trung binh cao (OM 2,85%), CEC trung blnh (12 meq/l00g dAt), phftn co gi6i trung blnh, ham IUQ'llg va C6ng ngh¢ Tdp 31 sa 2S (2015) 274-280 275 N, P, K t6ng s6 va d~ tieu giau, ham hrong Zn va Pb t6ng s6 97,23 ppm va 21,13 ppm, thftp han so voi quy chuan cho phep QCVN 03:2008IBTNMT Can cir vao mQt s6 kat qua nghien ciru tren th~ gioi va QCVN 03:2008IBTNMT dS gay nhiem dfrt Nghien ciru g6m kh6i thf nghiem b6 sung ham hrong Zn va Pb nrong dirong voi mire nhiem va rftt nhiem M6i thf nghiem g6m cong thirc (CT), m6i CT diroc I~p I~ Iftn, d6 CT d6i chirng duoc b6 trf chung cho kh6i thf nghiem, Thi nghiem 1: Nghien ciru anh huang ella Zn d~n cay keo neo, bao g6m cong thirc (1) BBi chirng CTO: khong b6 sung Zn, (2) CT 1.1: bB sung 80 ppm Zn, (3) CT 1.2: b6 sung 240 ppm Zn Thi nghiem 2: Nghien ciru anh huang cua Pb d~n cay keo neo, bao g6m cong tlnrc (1) eTO: tr6ng cay keo neo, khong b6 sung Pb, (2) CT 2.1: b6 sung 80 ppm Pb, CT 2.2: bB sung 240 ppm Pb Tian hanh chieu cao cay, d~m s6 la, diep luc la tai thoi di€m 10, 20, 30, 40 d~ nghien ciru anh huang ella Zn, Pb d6n sir phat triSn cua cay LAymiu thuc v~t a thoi diSm 20 va 40 de nghien ciru kha nang tfch lfiy Zn, Pb cac b{l ph~n than la, & PM miu dAt, thl,lc v~t theo phuang pMp chuftn va xac dinh Zn, Pb dung dich pM miu b~ng may quang phB hip ph1,lnguyen tir AAS Sir d1,lngphan m€m Excel xir 19 th6ng ke va rno ta sBli~u K~tqua nghien cUu 3.1 Anh hU07zgcua Zn din c{iy keo neo 3.1.1 Anh hU07zgcua Zn din chiiu cao cay K6t qua nghien ciru anh huang eua Zn d~n ehi€u eao cay keo neo dUQ'etrinh bay a bang eho thAy cac cong thuc thi nghi~m (CTTN) a r I 276 T.T.T Thu va nnk: /Tap chi Khoa hoc DHQGHN: Khoa hoc Til nhien va Cong ngh~, T~p 31, S6 2S (2015) 274-280 I i- khac chiSu cao city dSu tang len tai m6i thoi di€m (10 ngayll~n) Sau 10 ngay, chieu cao cay & cong thirc CTO, CTl.1 va CT1.2 tang tnroc thi nghiem, Tai thoi di~m 30, 40 chieu cao cay co xu huang tang nhanh & CTl.I va CT1.2, thAp nhAt CTO I~n hrot 138%, 119% va 115% so voi thoi di~m " " ,1 Bang Arm huong cua Zn d€n chieu eao cay keo nco (em) CITN CfO Cf1.l CT1.2 o thoi TruaeTN 10,93±1,73 1O,93±l,73 lO,93±1,73 10 ng!r 15,14±2,06 12,90±2,51 12,56±1,04 di~m 10 ngay, mire tang chieu cao cay dat gia tri cao nhAt tai CTO va thAp nhAt CT1.2, nhien sau 20 lai co xu huang giam din & CTO va tang len & CTl.1 va CT1.2 Sau 40 ngay, chien cao cay cao nhAt & CT1.21a 25,98 em, thAp nhAt & CTO la 23,92 cm Chirng to pham vi nghien ciru, Zn la nguyen t6 vi hrong co kha nang thtic dfty SlJ tang tnrong, lam tang chieu cao cua cay 20 17,51±2,07 16,11±2,08 16,55±l,93 30 ng!r 20,78±2,4 20,48±2,09 21,40±2,44 40 ng!r 23,92±2,98 24,88±2,01 25,98±2,65 " j 3.1.2 Anh huang cua Zn ddn sa La cua cay S6 la la mQt nhirng chi tieu quan d~ daub gia kha nang sinh tnrong cua cay S6 Ia tao dieu kien thuan IQ'icho qua trinh quang hop va trao d6i chAt t6t han K€t qua anh hirong cua Zn d~n s6 la cua cay keo neo duoc trmh bay & bang nhu sau: Bang Anh huong ella Zn d€n sf, hi cay CTTN CfO cn.i Cf1.2 TruaeTN 4-5 4-5 4-5 10 nga~ 4-5 3-4 20 nga~ 4-5 4-5 30 nga~ 4-5 4-5 40 nga~ 5-6 " Khi so sanh s6 hi & cac CTTN cung I khoang thoi gian, k~t qua nh~n thAy & thoi di~m 10 b6 sung Zn vao dAt, s6 Ia giam d~n tU CTO d~n CT1.2 thAp nhAt la CT1.2 hi Thoi di~m 20 va 40 ngay, s6Ia keo neo co xu huang tang len & cac cong thirc Sau b6 sung Zn diroc 40 thl cay keo neo co s6 Ia th~p nhAt & CTO la 5Ia va cao nh~t & CT1.2Ia Ia 3.1.3 Anh huang cua Zn din ham tU'971g di?p luc tang sa ld keo neo diep luc t6ng s6 co vai tro quy~t dinh den kha nang quang hop cua Ia cay, VI v~y tro quan d~n SlJ phat tri~n cua cay Bang Arm huong ella Zn d€n tAngdiep luc la keo nco (mg/cnr') CITN CfO Cfl.l Cf1.2 TruaeTN 0,023 ±0,05 0,019 ±0,09 0,022 ±0,02 10 0,025 ±0,06 0,016 ±0,08 0,02 ± Om 20 0,029 ±0,01 0,027 ± 0,Dl 0,033 ±0,02 30 0,033 ±O,OI 0,036 ± 0,Dl 0,04 ±0,o2 " i: 11 t Ham hrong se co vai j! 40 0,039 ±O,QI 0,042 ±0,02 0,043 ±O,Ol 1: 1, I:I T.T.T Thu va nnk: IT(lp chi Khoa hoc DHQGHN: Khoa hoc T7!nhien K6t qua nghien cuu duoc trinh bay (] bang cho th~y sau 10 thf nghiem, ham luong diep h.IC t6ng s6 hi keo neo (] CTl.1, CT1.2 co xu huong giam so voi thoi diSm tnroc thf nghiem, giam nhanh nh~t (] CTl.1 (giam 15,7%), nhien a CTO tang 109% so voi thoi diSm tnnrc thf nghiem va Ctmg ngh~, Tdp 31, sa 2S (2015) 274-280 277 Co thS nh~ th~y tai thoi diSm nay, ham luong diep h,IC t6ng s6 hi cay keo neo tj I~ thu~n voi ham hrong Zn duqc b6 sung vao dfrt 3.1.4 S" ticn lily Zn cay keo neo K~t qua nghien CUu anh huang cua Zn d6n su tfch liiy Zn than Ia va r€ cay keo neo (] thoi diSm 20 va 40 thf nghiem cho th~y, (] thoi diSm 20 ham hrong Zn tfch lily (] phAnr€ cua cay (] cac cong thirc CTO;CTl.1 va CT1.2 cao g~p 1,38; 1,88 va 2,34 IAnva a thoi diSm 40 cao g~p 1,62; 1,92 va 2,66 IAnso voi than Ia (Bang 4) Ham hrong diep h,IC t6ng s6 (] thoi diSm til 20 d6n 40 ngay, co xu huong tang (] ca cong thirc, tang nhanh nh~t a CTl.l (b6 sung 80 ppm Zn); thoi diSm40 ngay, dao dQng 0,039 - 0,043 mg/cnr', th~p nh~t (] CTO la 0,039 mg/cnr' va 160 nh~t (] CT1.2 la 0,043 mg/cm' Bang Tfch liiy Zn cay keo neo (ppm) CTIN 20 40 Than Ili Re Than CTO 66,16 91,24 157,48 55,54 89,92 145,46 CTl.1 99,36 186,36 285,72 99,3 190,52 289,82 CT1.2 115,6 270,12 385,72 113,56 302,14 415,7 Trong thoi gian 20 dan 40 ngay, ham luong Zn tfch lfiy than, Ia va r~ giam (] CTO Di~u dii cho th~y Zn la nguyen t6 dn thiet cho su phat triSn cua cay, thea thoi gian cay dn dinh dufrng d€ phat tri€n vi v~y rna ham luong Zn dii bi giam di o thoi diSm 40 ngay, t6ng ham hrong Zn tfch liiy than la r~ cay keo neo (] cong thirc CTl.1Ia 289,82 ppm (gAp 1,99 IAnd6i clnrng) va CT1.2Ia 415,7 ppm (g~p 2,86 IAnd6i chUng) Nhuu v~y vo; IUQ'IlgZn b6 sung cang cao thi kha nang tich liiy vao cay cang 160 lare Than la Re Than la re 3.2 Anh huang da Ph tI~ncay keo neo 3.2.1 Anh hutmg cua Ph tI~nchi~u cao cay Kat qua nghien ciru anh huang cua Pb dan chien cao cua cay keo neo duoc trinh bay bang cho th~y tai m6i thoi di€m quan tr&: (10 ngay/lAn) chieu cao cay keo neo cao nhAt (] cong tlnrc d6i clnrng, thApnhAt (] cong thirc b6 sung 240 ppm Pb Cu th€ la chi~u cao trung blnh clla cay cong thuc CTO> CT2.1 > CT2.2 a Bang AM huemg cua Pb dan chi6u cao cay keo neo (em) CTNT TruaeTN 10 20 30ngay 40 CTO 10,93 ± 1,73 14,14 ± 1,24 17,5 ± 1,45 20,28 ± 3,2 23,92± 2,03 CT2.1 10,93 ± 1,73 13,77 ± 2,2 16,62 ± 2,25 19,57 ± 1,56 22,52± 3,02 CT2.2 10,93 ± 1,73 12,73 ± 1,74 14,64 ± 2,03 16,65 ± 2,31 18,99 ± 2,09 " i 278 T.T.T Thu va nnk IT"p chi Khoa hoc DHQGHN: Khoa hoc Tv nhien SI,lthay d6i chieu cao cua cay cling co su nrong d6ng voi ham hrong Pb dk d giai doan 10 ngay, chien cao cay dat gia tri cao nhAt aCTO 13014,14 em va giam d~n CTI.1, CT2.2 va thAp nhAt a CT2.2 13012,73 em Tuy nhien sau thai di~m 20 ngay, mire dQ tang chieu cao cay dll tang thea nrng cong thirc thi nghiem va tang Ian nhAt aCTO thai di~m 40 chien cao cay Ian nhAt CTO la 23,92 em va thAp nhAtCT2.2 la 18,99 cm Nhu v~y pham vi nghien ciru nay, Pb dll anh huang den su tang T~p 31, S6 2S (2015) 274-280 tnrong cua cay keo neo thong qua lam giam chi~u cao cay 3.2.2 Anh huang eua Ph din sa a a va Ctmg ng~, u cua cay Ket qua nghien ciru duoc trinh bay bang cho thAyanh huang cua ham hrong cua Pb dAt va s6 la cua cay keo neo tY I~ nghich voi nhau, thea thai gian, bam hrong Pb dAtcang cao thl s6 hrong la tren cay cang giam, o Bang AM huang cua Pb d~n s6 hi cay(cm) CITN CfO CT2.1 Cf2.2 TnracTN 4 10 ngar 4-5 4-5 20 ngar 4-5 4 D6i voi cong thirc CTO khong b6 sung Pb, cay co Sl,l phat trien d~u den thai di~m 40 ngay, trung blnh - la, m6i cay tang 1- Ia so voi cac CTTN D6i voi CT2.1, CT2.2 chiu anh huang cua nhi~m Pb co s6 hi giam, giam ro nhAt a CT2.2 b6 sung 240 ppm Pb (Bang 6) Chirng to dng Pb dll anh huang den qua trinh sinh 1:9sinh hoa cay nen co hien nrong Ia Bang AM huang o 40 ngar 5-6 3-4 yang va rung la, lam giam s8 la keo neo CTTN so voi d6i chimg a cac 3.2.3 Anh huang eua Ph din ham luang di~p luc tang sa lei keo neo Ket qua duoc trinh bay bang cho thAy c6 m6i nrong quan tY I~ nghich gifra ham hrong hrong diep luc t6ng s6 la cay keo neo voi ham luong Pb duoc b6 sung vao dAt a cac CTTN so voi d6i chimg cua Pb d~n t6ng diep h,IC hi keo neo (mg/cnr') CTTN 10 20 CfO 0,025 ±0,06 0,029 ± om 0,03 ± 0,Dl 0,039 ±0,01 CT2.1 0,02 ±0,02 0,027 ± 0,01 0,03 ± 0,Ql 0,037 ± 0,01 CT2.2 0,018 ± 0,02 0,Q3 ± 0,04 0,Q3 ±O,04 0,Q3 ± 0,Q2 thai di~m 10 thi nghiem, ham hrong diep luc t6ng s6 Ia cay keo neo a CT2.1, CT2.2 co xu huang giam so voi truce thi nghiem, giam nhanh nhAt a CT2.2 (giam 30%) Trong do, cong thirc CTOchi tang it, b~ng 109% so v6'i truac thi nghi~m Thai di~m til 20 den 40 ngay, co xu huang tang a ca cong a 30 ngar 3-4 30 40 a thirc, tang nhanh nhAt CTO T~ thai di~m 40 ngay, thAp nhAt a CT2.2 la 0,03 mg/crrr' va Ian nhAt a CTO la 0,039 mg/cm' Ket qua nghien CUunay phil hop voi nghien CUucua Chadran va nnk (2009) nghien cUu b6 sung ham IUQllgPb til 10 dan 100 ppm vao dAtthi nghi~m va ti~n hanh thea doi thm gian 25 [1] T.T.T Thu vi! nnk IT(lp chi Khoa hoc DHQGHN: Khoa hoc Til nhien vi! Cong ngh?, Tdp 31, 3.2.4 S" ticb Lily Ph cay keo neo K~t qua nghien ciru (1 thoi di~m 20 va 40 gay nhi~m Pb cho thAyt6ng ham luong Pb tich h1y cay keo neo tang dan thea thoi S6 2S (2015) 274-280 279 gian va thea S\l tang ham hrong Pb cac CTIN, hrong Pb tfch lfiy r~ Ian hon than la (Bang 8) Bang Tfch liiy Pb cay keo neo (ppm) CTNf CTO CT2.1 CT2.2 20 nga~ Thanh! 0,17 0,23 0,29 R~ 0,22 0,32 0,64 Than hi, rS 0,39 0,55 0,93 o cong thirc CTO khong gay nhi€m Pb co ham hrong Pb tfch lily than va r€ tang nhe thoi gian 20 d~n 40 thoi di~m 40 ngay, t6ng ham hrong Pb tfch Illy than la r€ tai CT2.11a 4,76 ppm va CT2.21a 8,27 ppm, cao hon so vci d6i chimg 2,1 ppm (gAp 1,79 Ian) va 5,61 ppm (gAp 3,11 Ian) K~t qua chirng to r~ng tr6ng cay keo neo (1 giai doan cay non tren dAt nhi€m KLN, nau dung lam rau an co th~ lam tang nguy co gay tfch lfiy dQc chAtvao cay, ti~m frnnhirng rui ro d~n sire khoe ngiroi Tuy nhien, dirng (1 phirong dien dung thuc v~t d~ xu ly dAt0 nhi€m KLN thi keo neo nen duoc xem la d6i nrong dn quan tam nghien ciru o 4.K~tlu,n Nghien CUu tr6ng cay keo neo dAtgay o nhiem 80 ppm va 240 ppm Zn chua gay anh huang b~t 19i dan su phat tri~n cua cay rna anh huang tich C\lC dan tang chieu cao cay, s6 la, ham luong diep h,IC t6ng s6 laoNhtrng ciing (1 dAt co cung ham luong Pb 80 ppm va 240 ppm da gay anh huang bit 19i dan chieu cao cay, s6 la, ham luong di~p h.IC t6ng s6 la keo neo Khi ham hrong Pb diroc b6 sung vao dit cang cao thl gay anh huang tieu C\lC cho cay 40n~ Than hi 0,76 1,81 3,08 R~ 1,90 2,96 5,19 Than hi, r8 2,66 4,76 8,27 cang Ion, (1 ham hrong Zn duoc b6 sung thf nghiem chua co d~u hieu gay tac d9ng bAtloi dan qua trlnh phat tri~n cua cay keo neo Su tich liiyim, Pb r€ nhi~u hon than la va co quan M tY l~ thuan voi bam hrong Zn, Pb dtroc b6 sung vao dit Tal li~u tham khao [1] Chadran S and et al (2009), Studies on the Ecology, Distribution and Unilitarian aspects of Limnocharis flava Bunchenau, an invasive aquatic weed in Kuttand Wetland Ecosystem, School of Inviromental Sciences Mahatma Gandhi University, Mahatma Gandhi University, Priyadharshini Hills P.O., Kottayam- 686 008, Kerala, India [2] Kamarudzaman A N., Aziz R A., and Jalil M F (2005), Removal of Heavy Metals from landfill leachate Using Horizontal and Vertical Subsurface Flow Constructed Wetland Planted with Limnocharis flava International Journal of Civil & Enviromental Engineering, Volume 3, No [3] Abhilash P c Pandey V C., Srivastav P., Rakesh P S., Chandran S., Singh N., Thomas A P (2009), Phytofiltration of cadmium from water by Limnocharis flava Buchenau grown in free-floating culture system, Journal of Hazardous Materials, pp 791 - 797 [4] Anning A K., Korsah P E and Fordjour P.A (2013), Phytoremediation of wastewater with Limnocharis flava, Thalia geniculate and typha latifolia in Constructed wetlands, International Journal of Phytoremediation, pp 452-464 I 280 T.T.T Thu va nnk /T~p chi Klwa hoc DHQGHN: Klwa h9C tv nhien va Cong nghj, Tq.p31, sa 2S (2015) 274-280 Accumulations of Heavy metals (Pb, Zn) and their influence on Limnocharis Flava During the Nurseling Stage Trftn Thi Tuyet Thu 1, Nguyen Vi~t Hi~p2, Duong Thanh Van 'Faculty 0[' Environmental Sciences, VNU University of Science, 334 Nguyin Triii, Hanoi, Vietnam Soils and Fertilizers Research Institute, Dong Ngac, Tit Liem, Hanoi, Vietnam Abstract: Limnocharis flava is considered as one of the heavy metal hyperaccumulating plants It is wild or planted in the Mekong river delta and serves as a cooking ingredient for goby soup Seedlings (5 weeks old, - leaves, 10.93 em in height) were planted on soil in which contents of Zn and Pb were 80 and 240 ppm (respectively) and havested after 40 days Observations at 10, 20, 30, 40 days showed a positive effect of Zn on plant growth (height and chlorophyll contents) at its both contents of 80 and 240 ppm After 40 days, the heights of plants varied from 23.92 em (CfO) up to 25.98 em (in Cf1.2) Chlorophyll contents of the CTO and Cf1.2 were 0.039 and 0.043 mg/crrr', respectively In contrast, Pb at the same content as Zn showed negative effects on plant growth After 30-40 days, the presence of Pb resulted in remarkly decreases of the height and chlorophyll contents The heights of plants changed from 18.99 em (CT2.2) to 23.92 ern (CfO) The lowest chlorophyll content was 0.03 mg/cm" (Cf2.2), whereas and the highest one was 0.039 mg/cm" (CTO) Zn and Pb accumulated in the plant tisues showed a high correlation with the additive amounts, and they tend to translocate in roots rather than leaves and shoots The highest amounts of Pb and Zn were 415.7 and 8.27 ppm, while the lowest values were 145.46 and 8.27 ppm, respectively Keywords: Heavy metal, accumulation, zinc, lead, Limnocharis flava .j ... cong thuc thi nghi~m (CTTN) a r I 276 T.T.T Thu va nnk: /Tap chi Khoa hoc DHQGHN: Khoa hoc Til nhien va Cong ngh~, T~p 31, S6 2S (2015) 274-280 I i- khac chiSu cao city dSu tang len tai m6i thoi... 3,02 CT2.2 10,93 ± 1,73 12,73 ± 1,74 14,64 ± 2,03 16,65 ± 2 ,31 18,99 ± 2,09 " i 278 T.T.T Thu va nnk IT"p chi Khoa hoc DHQGHN: Khoa hoc Tv nhien SI,lthay d6i chieu cao cua cay cling co su nrong... nghi~m va ti~n hanh thea doi thm gian 25 [1] T.T.T Thu vi! nnk IT(lp chi Khoa hoc DHQGHN: Khoa hoc Til nhien vi! Cong ngh?, Tdp 31, 3.2.4 S" ticb Lily Ph cay keo neo K~t qua nghien ciru (1 thoi di~m