... pT7Blue2T:RS21 -C6( NdeI ⁄ BamHI) and pT7Blue2T:RS21 -C6( NcoI ⁄ BamHI), respectively. Construction of expression plasmids Plasmids pET3a:RS21 -C6, pET32a(+):RS21 -C6, pcDNA3.1- hyg(+):RS21 -C6 and pIRES2-EGFP:RS21 -C6 ... 17.4 (kDa) pcDNA pcDNA :RS21 -C6 pIRES2-EGFP pIRES2-EGFP :RS21 -C6 20 15 (kDa) RS21 -C6 GAPDH RS21 -C6 GAPDH 31.3 47.3 84.7 114 W N Mt Cy t RS21...
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