... Fractions 1, 3and 5 inhibited the reaction of PGM34 with the puri-fied mucin on the ELISA plate, with fraction 1 achiev-ing the strongest inhibition (Fig. 1B). Fractions 2 and4 produced almost the ... channels. Upon reaching the pit region, the zymogenic contents merge into the mucous neck cellmucous channel. The mucous-neck-cell-derived mucinis confined to the central portion of the glandularlumen, ... waschromatographed on two TSK-Gel Amide-80columns and eluted under isocratic condi-tions. The absorption was monitored at210 nm. The arrows indicate the elutionpositions of A1-5a and 5b. (B) The antigenicactivities...