Báo cáo y học: "Allele dependent silencing of COL1A2 using small interfering RNAs"

Ngày tải lên: 03/11/2012, 11:52

... Autophosphorylation of CCaMK resulted in time -dependent loss of enzyme activity (Figs 1 and 2; Table 1) and formation of a sedimentable enzyme (Fig. 3). Autophosphorylation -dependent loss of enzyme activity was ... Ca 2+ -stimulated autophosphory- lation of CCaMK results in time -dependent loss of enzyme activity. This time -dependent loss of activity or self-inacti- vatio...

Ngày tải lên: 17/03/2014, 17:20

... supplementary Tables S1–S12). For sev- eral mRNAs, we found small stretches of sequence identity with the 3¢-end of siRNA sense sequences (5¢-end of antisense sequences). However, only 65% of them (21 of ... designed using primer express 1.5 software (Applied Biosystems, Foster City, CA, USA). Amplification reaction assays con- tained SYBR Green PCR Mastermix (Applied Biosystems)...

Ngày tải lên: 07/03/2014, 05:20

... Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, China; 3 Protein Science Laboratory of the Ministry ... pathways involving the formation of distinct transition intermediates [1–3,7,8]. The complexity of the multiple pathways means that different mechanisms may occur with different types of...

Ngày tải lên: 21/02/2014, 01:21

... regardless of whether the yeast cultures are initiated in YPD, YPG or YPA. Interestingly, further analysis has shown that PtdEtn-PLD activity is also stimulated upon exit of yeast cells from stationary ... catalytic proper- ties. Its activity is Ca 2+ -dependent; it preferentially hydrolyses phosphatidylethanolamine (PtdEtn) and phos- phatidylserine (PtdSer); and its activity is not stim...

Ngày tải lên: 22/02/2014, 07:20

... respectively. 2Cys-Prx proteins are reduced by the AhpF protein in bacteria, and by the thioredoxin/thioredoxin reductase system in yeast and animals, w hile 1Cys-Prx may be r educed by a small thiol ... phenylthiohydantoin amino-acid analyser. F or MALDI-TOF analysis, 1 lL of tryptic digest or HPLC-puri®ed peptides was mixed with 1 lLof saturated solution of a-cyano-4-hydroxycinnamic a...

Ngày tải lên: 08/03/2014, 16:20

... on Conus carboxylase activity Our work [43] and that of Bandyopadhyay et al.[42] demonstrated the importance of the propeptide in directing c-carboxylation of Conus precursor substrates by the Conus carboxylase. ... with vitamin K -dependent carboxylase activity. Further, using insect cells, this expression system provides a source of Conus carboxylase free of endogenous carboxyl...

Ngày tải lên: 17/03/2014, 10:20

... activity of p25 rum1 as a Cdc2 kinase inhibitor The p25 rum1 protein is known to inhibit the kinase activity of a complex of Cdc2 and B type cyclin and thereby regulate the cell cycle of fission yeast. ... phosphorylation. The yeasts have been the favored organisms for investiga- tion of the basic biology, genetics, and biochemistry of the cell cycle [1]. Studies of the fission ye...

Ngày tải lên: 17/03/2014, 23:20

... curve analysis of BTC hydrolysis by recombinant wild-type human BuChE, by four selected mutants (Y3 32A/D70G, Y3 32D/D7 0Y, W82A, A32 8Y) and by commercial horse serum BuChE in the presence of TEA. ... the hydrolysis of BTC by this enzyme obeyed Michaelis–Menten kinetics. In order to investigate the kinetics of this mutant more closely, we measured the hydrolysis of BTC catalyzed...

Ngày tải lên: 24/03/2014, 03:21

Ngày tải lên: 25/10/2012, 10:02