Analysis, sequencing and in vitro expression of PCR products
Analysis, sequencing and in vitro expression of PCR products
... DNA footprinting and in situ PCR it is necessary to label the PCR product directly. Direct labeling of PCR Analysis, sequencing and in vitro expression of PCR products 101 products also increases ... (Chapter 4). In such 88 PCR Analysis, sequencing and in vitro expression of PCR products 5.1 Introduction Analysis of PCR products is c...
Ngày tải lên: 25/10/2013, 22:20
báo cáo hóa học: " Beyond platinum: synthesis, characterization, and in vitro toxicity of Cu(II)-releasing polymer nanoparticles for potential use as a drug delivery vector" ppt
... synthesis, and metal loading. RP conceived of the study, and participated in its design and coordination, carried out in vitro toxicity studies, SEM and EDX analysis and drafted the manuscript. Competing ... contained in the original loading solution deter- mined Cu loading, resulting in values ranging between 12 and 16 wt.% based on these reactions conditions. XPS was...
Ngày tải lên: 21/06/2014, 02:20
Báo cáo khoa học: Bridging the gap between in silico and cell-based analysis of the nuclear factor-jB signaling pathway by in vitro studies of IKK2 ppt
... simulated in silico. Combining this method of analysis (in vitro, cell-based and in silico analysis) will facilitate systematic understanding of the underlying properties of this signaling pathway. To ... 3E). Interestingly, the inclusion of IKK2 inhibition by SC-514 with our experimentally determined rate con- stants in the original model resulted in a delay in simu- la...
Ngày tải lên: 16/03/2014, 11:20
báo cáo hóa học:" Activation of PKA, p38 MAPK and ERK1/2 by gonadotropins in cumulus cells is critical for induction of EGF-like factor and TACE/ ADAM17 gene expression during in vitro maturation of porcine COCs" doc
... mechanism during in vitro maturation of porcine COCs. The intracel- lular mechanism of induction of the expression of EGF- like factor and TACE/ADAM17 further induce EGF-like factor and Tace/Adam17 mRNA expressions ... present study, we investigated which signaling pathways are involved in the gene expression of EGF-like factor and in Tace/Adam17 expression in...
Ngày tải lên: 20/06/2014, 07:20
ANALYSIS OF MICROBIAL COMMUNITY STRUCTURE AND IN SITU ACTIVITY OF NITRIFYING BIOFILMS
... Products Genetic Fingerprints Clone Libraries Sequence Database Probes Phylogenetic Tree Isolation Nucleic acid extraction RT -PCR PCR DGGE, TGGE or t-RFLP Cloning PCR Sequencing of clones Sequencing of individual ... RNARNA PCR ProductsPCR Products Genetic Fingerprints Clone Libraries Sequence Database Probes Phylogenetic Tree Isolation Nucleic acid extraction RT -PCR PCR D...
Ngày tải lên: 05/09/2013, 08:40
Tài liệu Báo cáo khoa học: Adaptive changes in the expression of nuclear and mitochondrial encoded subunits of cytochrome c oxidase and the catalytic activity during hypoxia pptx
... switch and alters the expression of the two nuclear genome coded isoforms of CytOX V [10,32]. The regulation of genes CytOX5a and CytOX5b, coding for the two isofoms Va and Vb, parallels that of ... not been investigated in detail. In a previous study we demonstrated that administration of inhibitors of heme biosynthesis (succinyl acetone and cobalt chloride) to mice...
Ngày tải lên: 20/02/2014, 23:20
Báo cáo khoa học: In vitro expansion of DNA triplet repeats with bulge binders and different DNA polymerases pdf
... by using 32 P-labeling primer or template in the presence or absence of DNA-binding agents (DDIs and doxorubicin). The DNA bulge binding of both compounds was detected by CD and UV melting experiments. ... the ligand–nucleic acid complex is formed. To gain insight into the binding of drugs to bulge DNA, several bulge- containing oligonucleotides were selected as binding hosts for...
Ngày tải lên: 07/03/2014, 06:20
Báo cáo khoa học: Insertion of the plant photosystem I subunit G into the thylakoid membrane In vitro and in vivo studies of wild-type and tagged versions of the protein doc
... 386 and 387, or at the extreme C-terminus of PSI-G (Fig. 1). These cassettes were both cloned into binary vectors under the control of the 35S promoter and terminator, and into vectors for in vitro ... com- plex (LHCI), consisting of four members (Lhca1–4) of the chlorophyll a ⁄ b binding protein family. LHCI is bound along one side of PSI only, in the vicinity of the...
Ngày tải lên: 07/03/2014, 21:20
In Vitro Screening of Plant Resources for Extra-Nutritional Attributes in Ruminants: Nuclear and Related Methodologies pptx
... and H. Hoste Fig. 3.4 Illustration of the fitting of the mesh and collar to the well 9. Carefully remove the filters and wash any remaining larvae into individual labelled petri dishes and stain ... the fibre and lignin concentration of leaves of a range of fodder trees when compared to air and freeze-drying. There was also a reduc- tion in soluble tannins, total nitrogen...
Ngày tải lên: 15/03/2014, 04:20
Báo cáo khoa học: Chick embryo anchored alkaline phosphatase and mineralization process in vitro Influence of Ca 2+ and nature of substrates doc
... C.G., Aubin, J.E. & Heersche, J.N.R. (1991) Initiation and progression in mineralization of bone nodules formed in vitro: the role of alkaline phosphatase and organic phosphate. Bone Mineral ... Materials and methods. Ó FEBS 2003 Bone GPI-bALP, roles in mineralization (Eur. J. Biochem. 270) 2087 Chick embryo anchored alkaline phosphatase and mineralization process in...
Ngày tải lên: 23/03/2014, 17:21