Analysis of gene expression
Analysis of gene expression
... levels of different gene transcripts. Changes in gene expression of even more genes can be analyzed in parallel by the use of microarrays which can allow several tens of thou- sands of gene probes ... genome and regulation of transcriptional processes leads to differential expression of cell-specific genes during development. In modern biology, accurate analysis of...
Ngày tải lên: 25/10/2013, 22:20
Báo cáo khoa học: Modified PCR methods for 3¢ end amplification from serial analysis of gene expression (SAGE) tags doc
... (2005) Analysis of long-lived C. elegans daf-2 mutants using serial analysis of gene expression. Genome Res 15, 603–615. 10 Ryu EJ, Angelastro JM & Greene LA (2005) Analysis of gene expression ... Development of these methods has promoted the wide- spread use of the SAGE technique, and has accelerated the speed of studies of large-scale gene expression. Abbr...
Ngày tải lên: 07/03/2014, 00:20
Báo cáo khoa học: Semi-nested PCR analysis of unknown tags on serial analysis of gene expression potx
... transcription–PCR analysis of unknown serial analysis of gene expression tags; rSAGE, reverse serial analysis of gene expression; SAGE, serial analysis of gene expression; TSAT-PCR, two-step analysis of unknown ... as a method to identify genes expressed in different cell types. Abbreviations GLGI, generation of longer cDNA fragments from serial analysis of...
Ngày tải lên: 07/03/2014, 04:20
Tài liệu Báo cáo khoa học: The splicing factor ASF/SF2 is associated with TIA-1-related/ TIA-1-containing ribonucleoproteic complexes and contributes to post-transcriptional repression of gene expression doc
... control of gene expression. As each step of the RNA metabolism is tightly regulated, the regulation of mRNA export, stability and transla- tion rate is essential for the control of the expression of ... characterization of TIAR-TAP protein and purification of TIAR-TAP complexes. (A) Upper panel: RT-PCR analysis of exon 6A inclusion in minigene reporter transcript upon ov...
Ngày tải lên: 16/02/2014, 15:20
Impact of Gene Expression Profiling Tests on Breast Cancer Outcomes potx
... design. Sources of Variability in Gene Expression Analysis Gene expression analysis poses several general challenges that can affect the reproducibility and reliability of the measurements ... the analysis of the transcriptome. 13 Investigators have developed approaches to gene expression analysis that have led to substantial advances in our understandin...
Ngày tải lên: 06/03/2014, 01:20
Báo cáo y học: " Transcriptome analysis of murine thymocytes reveals age-associated changes in thymic gene expression"
... Cell Quest software (BD). Results Gene expression changes were identified by comparing gene expression profiles across the indi-cated age groups to the gene expression profiles of the 1 month ... 2. Expression profile of the most sig-nificantly changed genes with age. This profile was generated in the NHGRI website (https://arrayanalysis.nih.gov), using dis-tance-based anal...
Ngày tải lên: 03/11/2012, 11:35
Báo cáo khoa học: Isolation, characterization and expression analysis of a hypoxia-responsive glucose transporter gene from the grass carp, Ctenopharyngodon idellus potx
... hybridization for normalization of gcGLUT expression. Phylogenetic analysis Phylogenetic analysis was performed by maximum parsi- mony using the PROTPARS program of the PHYLIP package version 3.57c ... responsiveness of these genes. Here, we describe the cloning and genomic structure of a hypoxia-responsive glucose transporter gene, gcGLUT from the grass carp and the characteri...
Ngày tải lên: 17/03/2014, 03:20
Báo cáo khoa học: Genomic structure and expression analysis of the RNase j family ortholog gene in the insect Ceratitis capitata pptx
... the general genomic organization of this gene and analyzed its structural evolution. Results Expression analysis of Cc RNase In our previous work, we reported the cloning and overexpression of ... organization and molec- ular evolution of the RNase j gene from various animal species, as well as expression analysis of the ortholog gene in C. capitata. The high degree o...
Ngày tải lên: 23/03/2014, 06:20
Báo cáo khoa học: Analysis of dopamine transporter gene expression pattern ) generation of DAT-iCre transgenic mice doc
... structure of the DAT gene is shown. The DAT gene has 14 exons (rectangles). The recombination targeted the second DAT exon, inserting the Cre transgene at the level of the ATG of the DAT gene. The ... the control of the regulatory elements of the dopamine transporter gene, for investigations of gene function in dopaminergic neu- rons. The codon-improved Cre recombinase (iC...
Ngày tải lên: 30/03/2014, 08:20
Báo cáo khoa học: Characterization and expression analysis of the aspartic protease gene family of Cynara cardunculus L. docx
... region of the cardosin A gene allowed the identification of a region of approximately 500 bp essential for gene expression in transgenic flowers. Additionally, the relevance of the lea- der intron of ... positive regulatory element of cardosin B gene pistil expression. Future work on transgene expression driven by a motif III-mutated leader intron of the cardosin B gen...
Ngày tải lên: 30/03/2014, 09:20