... (2005) Analysis of long-livedC. elegans daf-2 mutants using serial analysis of gene expression. Genome Res 15, 603–615.10 Ryu EJ, Angelastro JM & Greene LA (2005) Analysis of gene expression ... Development of these methods has promoted the wide-spread use of the SAGE technique, and has accelerated the speed of studies of large-scale gene expression. AbbreviationsGLGI, generation of longer ... for gene identification; Ptag, tag-specific primer; RAST-PCR, rapid RT-PCR analysis of unknown SAGE tags; rSAGE, reverse SAGE; SAGE, serial analysis of gene expression; TSAT-PCR, two-step analysis...