... various amounts of sodiumpyruvate, 5 g of ammonium acetate, 0.6 g of pyrocatechol, 0.2 g of sodiumsulfite,0.1 g of EDTA, and cells harvested from 100 ml of broth, in a total volume of 100 ml.The ... The mutant strain of E. coli which lacksl-proline-degrading enzyme was obtained and used for the host cells of produc-tion of l-hydroxyproline.Microbial Production of Amino Acids in Japan83Using ... successfulintroduction of the technology, various methods were searched for and deve-loped for microbial production of other amino acids. Today a whole array of amino acids are produced by microbial...