Interactions of Bioactive Plant Metabolites - Synergism, Ant

Interactions of Bioactive Plant Metabolites - Synergism, Antagonism, and Additivity

... Chapter 10 Interactions of Bioactive Plant Metabolites: Synergism, Antagonism, and Additivity John Boik, Ara Kirakosyan, Peter B. Kaufman, E. Mitchell Seymour, and Kevin Spelman Abstract ... of this chapter to refer to both approved drugs and bioactive plant compounds. The binding of a drug to a protein may affect the function of that protein through a number o...

Ngày tải lên : 25/10/2013, 05:20

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Tài liệu Báo cáo khoa học: Substrate speciﬁcity and inhibition of brassinin hydrolases, detoxifying enzymes from the plant pathogens Leptosphaeria maculans and Alternaria brassicicola ppt

... methyl N -( 3-indolylmethyl)carbamate (11), N -( 3-indolylmethyl)-N¢¢-me- thylurea (12), N -( 3-indolylmethyl)-N¢¢-methylthiourea (13), ethyl N -( 3-indolylmethyl)dithiocarbamate (14), N¢-acetylindolyl-3-methanamine ... buffer (pH 7.5) and 10 lLofN-ethyl-5-phenylisoxazolium-3 - sulphonate (Woodward’s reagent K; 10 mm), N-( 3- dimethylaminopropyl)-N¢-ethylcarbodiimide (10 mm)...

Ngày tải lên : 18/02/2014, 14:20

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Tài liệu Báo cáo khoa học: Mutational analysis of plasminogen activator inhibitor-1 Interactions of a-helix F and its neighbouring structural elements regulates the activity and the rate of latency transition pdf

... giving indistinguishable results: wild-type, PAI-1(T96A), PAI- 1(F100A), PAI-1(V126A), PAI-1(F128A), PAI-1(I137A), PAI-1(I138A), PAI-1(N139A), PAI-1(W141A), PAI- 1(T146A) and PAI-1(M149K). Structural analysis Structural ... composition of the hF/s3A-loop and hF plays a role in the stressed-to-relaxed transition of PAI-1: The hF/s3A-loop must fold away from b-sheet A during the structu...

Ngày tải lên : 20/02/2014, 11:20

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Báo cáo khoa học: C-terminal, endoplasmic reticulum-lumenal domain of prosurfactant protein C – structural features and membrane interactions ppt

... vesicles; POPC, 1-palmitoyl-2-oleoyl-phosphatidylcholine; POPE, 1-palmitoyl-2-oleoyl- phosphatidylethanolamine; POPG, 1-palmitoyl-2-oleoyl-phosphatidylglycerol; T m , gel-to-ﬂuid phase transition ... part of prosurfactant protein C (proSP-C) and is a-helical in its native state. The C-terminal part of proSP-C (CTC) is localized in the endoplasmic reticulum lumen and binds to misfo...

Ngày tải lên : 07/03/2014, 05:20

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Báo cáo khoa học: Interactions of HIPPI, a molecular partner of Huntingtin interacting protein HIP1, with the speciﬁc motif present at the putative promoter sequence of the caspase-1, caspase-8 and caspase-10 genes pdf

... incorporated on the basis of this. Detection of nuclear fragmentation, and caspase-1, caspase-3 and caspase-8 activation Nuclear fragmentation, and caspase-1, caspase-3 and caspase-8 activation, were ... binding assay of pDED of HIPPI with the caspase-1 gene upstream sequences. In panel I, typical results of EMSA with the 717 bp caspase-1 probe and 6X(HN)-pDED of Hippi...

Ngày tải lên : 07/03/2014, 10:20

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Báo cáo khoa học: Identiﬁcation, subcellular localization and functional interactions of PilMNOWQ and PilA4 involved in transformation competency and pilus biogenesis in the thermophilic bacterium Thermus thermophilus HB27 ppt

... very similar to the C-termini of other members of the secre- tion family, such as PilQ of Myxococcus xanthus [28], ExeD of Aeromonas salmonicida [29], PilQ of P. aerugi- nosa [25,30], and PilQ of N. gonorrhoeae ... IV pili of Gram-negative bacteria. Like PilO and PilN of Pseudomonas aerugi- nosa and Neisseria gonorrhoeae [25–27], the T. thermophilus PilO and PilN pro...

Ngày tải lên : 07/03/2014, 12:20

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Báo cáo khoa học: Differential interactions of decorin and decorin mutants with type I and type VI collagens pptx

... ﬂow rate of 10 lLÆmin )1 of HBS was maintained. The surface of the chip was activated by injecting a mixture of equal volumes of 0.2 M N-ethyl-N -( 3-dimethylaminopropyl )- carbodiimide and 0.05 M N-hydroxysuccinimide. ... structure between the wild-type decorin and the mutants. The CD spectra of wild-type decorin and DCN E180Q and D CN E180K appeared similar, whereas...

Ngày tải lên : 07/03/2014, 16:20

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Báo cáo khoa học: Insertion of the plant photosystem I subunit G into the thylakoid membrane In vitro and in vivo studies of wild-type and tagged versions of the protein doc

... Position of the His-tag (HHHHHH) and the Strep-tag (WSHPQFEK) in either the loop region or the C-terminus of PSI-G is indicated. HisT, PSI-G-HisTerm; StrepT, PSI-G-StrepTerm; HisL, PSI-G-HisLoop; ... the C-terminally and loop-tagged versions of PSI-G are present in PSI, sug- gesting that all versions of PSI-G can be incorporated into the PSI complex. However, C-terminally His- an...

Ngày tải lên : 07/03/2014, 21:20

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Báo cáo khoa học: Puriﬁcation of a plant nucleotide pyrophosphatase as a protein that interferes with nitrate reductase and glutamine synthetase assays pdf

... motif (Arg-Ser-X- phosphoSer- X-Pro) that is recognized by and binds to NIP (nitrate reductase inhibitor protein) [3,4], which comprises isoforms of 1 4-3 -3 proteins [5,6]. Binding of 1 4-3 -3 proteins inhibits ... donor insteadofNADH;andinhibitedtheoxidationofNADHby both spinach NR and an Escherichia coli extract in a time- dependent manner. The apparent inhibition of GS an...

Ngày tải lên : 08/03/2014, 08:20

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Báo cáo khoa học: Intrinsic local disorder and a network of charge–charge interactions are key to actinoporin membrane disruption and cytotoxicity ppt

... the membrane. Materials and methods Expression and puriﬁcation of StnII-R29Q and StnII-Y111N mutants The unlabelled StnII-R29Q and the double uniformly labelled 13 C ⁄ 15 N StnII-R29Q and 13 C ⁄ 15 N StnII-Y111N samples ... in a b-barrel like those in the wild-type protein and StnII-R29Q mutant (Figs 1 and 2). In addition, the structure of StnII-Y111N shows two additional sh...

Ngày tải lên : 14/03/2014, 23:20

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