... Whileacetone treatment induced FITC–VT1B glomerular binding in type A samples, binding in type B samples was onlyslightly induced, indicating a robust distinction in terms of the Gb3parameters ... whereHRP, staining blue, was used to detect VT1 binding, and AP, staining red, was used to detect either VT2 or anti-Gb3binding. Immunolabelling resulted in a purple stain whereVT1 and anti-Gb3colocalized. ... anti-Gb3colocalized. In the majority of samples,the staining of anti-Gb3 and VT1 corresponded. In somecases, however, tubular staining by anti-Gb3was distinctfrom that of VT1 and this varied...