Mode sensors and switches - P3
Glycoprotein Methods and Protocols - P3
... presence of competitive inhibitors of O-glycosylation, such as benzyl 2-acetamido-2-deoxy-α-D-galactopyranoside, without adversely affecting cells (9). With the eluci-dation of more sequence data for ... on the blot (e.g., 3,3'-diaminobenzidine, 4-chloronapthol mix forHRP [14]; 5-bromo-4-chloro-3'-indolyphosphate toluidine salt, nitro blue tetrazolium chlo-ride mix for AP). Alternat...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P30
... supernatant and count the radioactivity.3.1.3. Glycoprotein Assay with β 1-3 Linked- and β 1-4 Linked-GalactoseRadioactive Substrates (seeNotes 9, 10, and 14)The detection of β 1-3 - and β 1-4 -specific ... discriminationbetween Galβ 1-3 GalNAc and Gal products should be made. In the case of the synthetic sub-strate Galβ 1-3 GalNAc-4-nitrophenyl it is possible that the...
Ngày tải lên: 08/10/2012, 10:25