... hybridization o feither PstI(lanea)orEcoRI (lane b) digested c hromosomal DN A in c ase of sipV or Hin dIII (lane a) or EcoRI-SacI (lane b) digested genomic DNAin case of sipW. Hybridization indicated ... autolysin activities of wild type cells, which runs in adouble band of proteins of about 35 kDa was missing.Nuclease activitiesOccasionally we observed that after plasmid isolation higheryields ... mutants,including c ell autolysis, motility, sporulation, and nucleaseactivities, seemed to correlate with speci®cities and/orlocalization o f B. amyloliquefaciens SipS, SipT and SipVisoforms.Keywords:SignalpeptidaseI;Bacillus...