Nitrogen Acquisition and Amino Acid Metabolism

... between nitrogenase reductase and a nitrogenase ab dimer Within the nitrogenase reductase (shown in green and yellow here), the Fe4S4 center is closest to the nitrogenase ab dimer The nitrogenase ... kinase Amino Acid Biosynthesis • Plants and microorganisms can make all 20 amino acids and all other needed N metabolites • In these organisms, Glu is the source of N, via transamin...

Ngày tải lên: 15/06/2017, 20:30

... serine and glycine was reduced from 33% in the light to 25% in the dark (Fig 7A) Glutamine and asparagine were the major amino acids in the xylem sap in both the light (63% of the total amino acids) ... 3195 Amino acid metabolism and translocation M.-H Valadier et al Table Amino acid composition in leaves, and amino acid percentage ratio i...

Ngày tải lên: 18/02/2014, 18:20

... 4¢-phosphopantothenoylcysteine synthetase CoaB (Eur J Biochem 271) 169 Fig Analysis of the enzymatic activity of His -CoaB R206Q and A276V The synthesis of 4¢-phosphopantothenoyl-CMP (–cysteine, upper part of the figure) and ... important for the second-half reaction and are part of the active-site of CoaB; these residues may be directly involved in bind...

Ngày tải lên: 19/02/2014, 12:20

... with Co2þ as the cofactor [26] The class II Bacillus coagulans XI, on the other hand, isomerizes xylose most efficiently when in the presence of Mn2þ, whereas its activity toward fructose is best ... responsible for the thermostability of Thermus thermophilus isopropylmalate dehydrogenase Biochim Biophys Acta 1545, 174–183 Petsko, G.A (2001) Structural basis of thermosta...

Ngày tải lên: 22/02/2014, 07:20

... Janecek Origin of rBAT and 4F2hc within the GH13 a-amylase family Fig The CSRs of the hcHAT proteins and the GH13 a-amylase family members A list of the abbreviations of proteins and enzymes can ... identifiable, even for the enzymatically active GH13 members [13] Therefore, one of the goals was to align Origin of rBAT and 4F2hc with...

Ngày tải lên: 18/02/2014, 13:20

... Kumagaye KY, Nakajima K, Watanabe T, Kawai T, Kawakami Y, Niidome T, Sawada K, Nishizawa Y et al (1994) Omega-agatoxinTK containing D-serine at position 46, but not synthetic omega-[L-Ser46]agatoxin-TK, ... Inoue A, Kawakami Y, Nishizawa Y, Katayama K & Kuwada M (1995) Isolation and characterization of a peptide isomerase from funnel web spider venom J Biol Chem 270, 16719–16723 To...

Ngày tải lên: 18/02/2014, 17:20

... et al Staphylococcal nuclease refolding investigated Two point-mutated proteins, with a single base substitution of alanine for tryptophan (W14 0A) and alanine for lysine (K13 3A) , and two truncated ... both the wild-type protein and mutants E142O and K13 3A Thermal analysis of protein unfolding The DSC curves of the wild-type protein and the mutants K13 3A,...

Ngày tải lên: 20/02/2014, 01:20

... substitution mutant snake DNases I The thermal stabilities of the wild-type and mutant enzymes of the snakes were examined by measuring the activities remaining after incubation for 40 at various ... mechanism of generating a thermally stable enzyme form from a thermally unstable one: frog, toad and newt DNases I all have a Ser205 insertion in a domai...

Ngày tải lên: 20/02/2014, 23:20

... Both transitions to latent and substrate forms were strongly but differently influenced by the amino-acid composition of the shutter region Surprisingly, we found that substitution of Lys335 to ... investigate the importance of the shutter region for the unique types of RCL insertion in PAI-1, we have now undertaken a number of substitutions in...

Ngày tải lên: 22/02/2014, 07:20

... devoid of a given essential amino acid Our data suggest that the GCN2 pathway is directly involved in the regulation of amino acid and protein metabolism, as many of the genes involved in these processes ... their biological processes reveals that amino acid limitation regulates groups of genes that are involved in amino acid and pro...

Ngày tải lên: 07/03/2014, 03:20

... corresponding to the introns of the two genes The fragments were sequenced and enabled the complete reconstruction of the Psy1 and Psy2 genes with the annotation of introns and exons The GenBank ... based on the lack of information regarding the 5¢-region in the Psy2 DNA sequence and from the conflicting evidence available in the database records of t...

Ngày tải lên: 07/03/2014, 05:20

... transporter to the internal membrane side is the rate-limiting step and not – as proposed for most of the other electrogenic symporters – the return of the unloaded transporter to the outside of the membrane ... interpreted as the first evidence for a restricted velocity in the translocation step of the loaded transporter by the sterical conformation of...

Ngày tải lên: 07/03/2014, 16:20

... Local stability and key acidic amino acids in staphylococcal nuclease by the interactions of E75 with H121 and K97 It is believed that a small number of key amino acid segments play major roles ... 44% of E129G), respectively, lower DHcal values than the wild-type protein These outcomes indicate that little in uence is exerted by Gly 3969 Local stabilit...

Ngày tải lên: 07/03/2014, 21:20

... control at t ¼ for each peptide The percentage of degradation was calculated by comparing the area of the peaks of the intact peptide at t ¼ and t ¼ 60 Binding assays Binding assays were carried ... [33], the peptides containing a b-amino acid substitution in position have increased stability compared to the corresponding a- amino -acid- containing peptides...

Ngày tải lên: 08/03/2014, 08:20

... towards N- acetyl-D-methionine, N- acetyl-D-alanine, N- acetyl-D-leucine and N- chloroacetyl-D-phenylalanine than towards N- acetyl-D-valine, N- acetyl-D-phenylalanine, N- acetyl-D-tryptophan, N- acetyl-D-tyrosine ... A- 6-D50061: Alcaligenes xylosoxydans ssp xylosoxydans A- 6 N- acylD-glutamate amidohydrolase; Alicaligenes faecalis-DA1: Alcaligenes faecalis DA1 N- acyl D-amino...

Ngày tải lên: 08/03/2014, 16:20