Ebook Introduction to modern cryptography Part 2

... also U-HPLC High-pressure liquid chromatography, High-priced liquid chromatography; see U-HPLC High-speed liquid chromatography, HILIC, see Hydrophilic interaction chromatography Homologs, retention, ... rules, 583–584 structure vs chromatography, 583–584 Proteomics, multidimensional liquid chromatography, 616–618 Protocol; see Validation, protocol Pseudocritical chromatograp...

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... INTRODUCTION 1. 1 Background Information, 1. 1 .1 What Is HPLC?, 1. 1.2 What Can HPLC Do?, 1. 2 A Short History of HPLC, 1. 3 Some Alternatives to HPLC, 1. 3 .1 Gas Chromatography (GC), 1. 3.2 Thin-Layer Chromatography ... Information, 12 1. 4 .1 Books, 12 1. 4.2 Journals, 13 1. 4.3 Reviews, 13 1. 4.4 Short Courses, 13 1. 4.5 The Internet, 13 References, 15 BASIC CON...

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... 5.1 Introduction, 20 0 5 .2 Column Supports, 20 0 5 .2. 1 Particle Characterization, 20 1 5 .2. 1.1 Particle Type, 20 1 5 .2. 1 .2 Particle Size and Pore Diameter, 20 3 5 .2. 2 Silica Supports, 20 3 5 .2. 2.1 ... Efficiency, 20 5 5 .2. 2 .2 Nature of the Silica Surface, 20 8 5 .2. 2.3 Particle Preparation, 21 1 5 .2. 3 Porous Polymers, 21 2 5 .2. 4 Monoliths, 21 2 5 .2. 4.1 Sil...

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... Polymer 13. 10.4 Analysis, 6 53 13. 10 .3. 1 Size-Exclusion Chromatography, 6 53 13. 10 .3. 2 Interactive Liquid Chromatography, 6 53 13. 10 .3. 3 Liquid Chromatography under Critical Conditions, 655 13. 10 .3. 4 ... 730 15.2.1 Columns, 730 15.2.2 Sample Introduction, 731 15.2.2.1 Loop Injectors, 731 15.2.2.2 Pump Injection, 732 15.2 .3 Detectors, 733 15.2 .3. 1 UV Detectors, 733...

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... ion-exchange chromatography ion-pair chromatography retention factor (same as capacity factor k ); equal to (tR /t0 ) − gradient retention factor; Equation (9.5) column length (mm) liquid chromatography–mass ... chromatographers as students Second, all three editions have tried to combine practical suggestions (‘‘how to? ’’) with a theoretical background (‘‘why?’’) Both theory and prac...

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... 11 15 14 10 20 60 (sec) 50 40 30 (b) 22 37 38 63 67 34 12 41 30 46 48 32 39 57 24 42 47 51 71 13 31 36 50 43 49 23 33 55 59 66 72 35 28 82 68 65 58 44 77 19 29 56 60 12 16 69 7376 45 52 73 85 61 ... Introduction to Modern Liquid Chromatography, Third Edition, by Lloyd R Snyder, Joseph J Kirkland, and John W Dolan Copyright © 2010 John Wiley & Sons, Inc INTRODUCTION...

Ngày tải lên: 04/07/2014, 01:20

... chromatograph by Waters Associates in the early 1 960 s [ 16] Carl Runge, a German dye-chemist born in 18 56, first reported crude dye separations by means of a technique similar to paper chromatography ... the early days of HPLC the technique was sometimes referred to as ‘‘high-pressure liquid chromatography’’ or ‘‘high-speed liquid chromatography, ’ for reasons suggested by Figure 1...

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... Separation, 70 2.6.2 Mass Overload: Effect of Sample Weight on Separation, 71 2.6.3 Avoiding Problems due to Too Large a Sample, 73 2 .7 RELATED TOPICS, 74 2 .7. 1 2 .7. 2 2 .7. 3 2 .7. 4 2 .7. 5 2 .7. 6 2 .7. 7 Column ... REFERENCES 17 46 H Yin and K Killeen, J Sep Sci., 30 (20 07) 14 27 47 J S Fritz and D T Gjerde, Ion Chromatography, 3rd ed., Wiley-VCH, Weinheim, 2000 48 J Weis...

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... polarizability 2.3 R E T E N T I O N (a) 31 (b) sample molecule H CH3−CH2−CH2−CH2−CH2−CH3 C 18 C 18 C 18 C 18 C 18 C 18 C 18 Nonpolar (hydrophobic) interaction with the nonpolar stationary phase (c) HO− CH2−CH2−CH2 ... around the nucleus of atom S will be unsymmetrical at any instant of time (as in Fig 2.8a), and this will cause the electrons in adjacent atom X to move as shown (due...

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... a chromatogram, Equation (2.10) tells us that peak width W will increase in proportion to retention time A continual increase in peak width from the beginning to the end of the chromatogram is ... times A decrease in particle size generally leads to an increase in N, as seen in Figure 2.11a The occurrence of maximum N is seen to occur at higher flow rates for smaller particles, which a...

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... SEPARATION (a) 106 P = 2000 psi dp = 10 mm mm 105 mm N 104 103 0.1 10 1000 t0 (min) (b) t0 = 100 0 sec t0 /N (sec) 10 1 10 2 dp = 10 mm mm mm 10 3 t0 = 10 sec 10 4 10 103 t0 = 100 sec 104 N 105 106 Figure ... CONTROL OF SEPARATION P = 2000 psi N = 106 105 Run time t (sec) N = × 105 104 dp = 10 μm hr N = 105 103 dp = 1.5 100 N = × 104 dp = 10 N = 5000 N = 10...

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... many laboratories have preferred columns packed with -5μm particles Such columns are less demanding in terms of equipment (Section 3.9), and are less likely to be plugged by particulates Today, however, ... can see from these two chromatograms that an intermediate value of %B is likely to improve resolution, by moving peaks and apart—before peak-pairs 1/2 or 5/6 come together In fact a mo...

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... another peak in the chromatogram Poor batch -to- batch reproducibility of the column is today an infrequent problem It is more likely to arise for complex samples where the chromatogram is crowded and ... reasons: • to avoid an undesirable change in separation due to a sample size that is too large • to increase detection sensitivity for trace analysis, by using the largest possible s...

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... Snyder and J J Kirkland, Introduction to Modern Liquid Chromatography, 2nd ed., Wiley-Interscience, New York, 1979, pp 34–36 46 Reviewer Guidance Validation of Chromatographic Methods, Center ... High-Performance Liquid Chromatography Advances and Perspec´ tives, Vol 2, C Horvath, ed., Academic Press, New York, 1980, p 24 S G Weber and P W Carr, in High Performance Liquid Chrom...

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