... Establishment of stable transfectants and the luciferase assay An aliquot of 0.4 lg of each plasmid was transfected into · 105 HeLa cells with lipofectamine2000 (Invitrogen, Carlsbad, CA, USA) according ... 14646–14652 Ishii A, Ohta M, Watanabe Y, Matsuda K, Ishiyama K, Sakoe K, Nakamura M, Inokuchi J, Sanai Y & Saito M (1998) Expression cloning and functional characterization of human cDNA for ganglioside ... of Gb3 markedly decreased, which was followed by an accumulation of LacCer and the appearance of another GSL (Fig 6A, asterisk) Based on the HPLC elution time (Fig 6B, asterisk), it seems that...