Glycoprotein Methods and Protocols - P30

... supernatant and count the radioactivity.3.1.3. Glycoprotein Assay with β 1-3 Linked- and β 1-4 Linked-GalactoseRadioactive Substrates (seeNotes 9, 10, and 14)The detection of β 1-3 - and β 1-4 -specific ... asialo-ovinesubmandibular gland mucin, or α1-acid glycoprotein, in 500 µL of 50 mM sodium phos-phate and 5 mM NaCl, pH 7.0.2. Add 5 U of galactose oxidase (Sigma) to...

Ngày tải lên: 08/10/2012, 10:25

... T-antigen (β-d-Gal 1-3 GalNAc),but also to structures found within the backbone of oligosaccharides (β-d-Gal 1-3 /4GlcNAc) (19). Demonstration of PNA binding is not necessarily evidence of T-anti-gen ... mounting ,and its relative stability. There are, however, several other alternatives, such as 3-amino-9-ethylcarbazole (AEC), for use with horseradish peroxidase (HRP); 5-bromo-4-chloro-...

Ngày tải lên: 08/10/2012, 10:25

... presence of competitive inhibitors of O-glycosylation, such as benzyl 2-acetamido-2-deoxy-α-D-galactopyranoside, without adversely affecting cells (9). With the eluci-dation of more sequence data for ... specificity (nonmucin glycoproteins can react, no carbohydrate or core pro-tein specificity), and lower sensitivity than slot-blot and immunoassay methods. Chap- Detection and Quantita...

Ngày tải lên: 08/10/2012, 10:25

... with“naked” cysteine-rich domains (4–7).Histological and biochemical investigations suggested that mucous-producing tis-sues and their secretions contained a complex mixture of mucin-type glycoproteins.However, ... prepared fromthe gel-forming mucins isolated from other epithelial secretions (respiratory and cer-vical) and from intestinal cell lines (HT-29 and PC/AA). In this exampl...

Ngày tải lên: 08/10/2012, 10:25

... Heterogeneity and Size Distribution of Gel-Forming Mucins 8787From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The MucinsEdited by: A. Corfield ... MUC5B, and MUC6 (1–4)—are gel-forming mucins, and it is possiblethat there are others. In isolating a mixture of mucins from mucus, there is consider-able scope for heterogeneity in their mass and...

Ngày tải lên: 08/10/2012, 10:25