Glycoprotein Methods and Protocols - P27
Glycoprotein Methods and Protocols - P27
... sequenceaMUC1VNTRp 1-4 0 PDTRPAPGSTAPPAHGVTSAPDTRPAPGSTAPPAHGVTSAp 1-2 4 PDTRPAPGSTAPPAHGVTSAPDTRp 5-2 0 PAPGSTAPPAHGVTSAp1 3-3 2 PAHGVTSAPDTRPAPGSTAPC-p1 3-3 2 (C)PAHGVTSAPDTRPAPGSTAPp 1-1 5 PDTRPAPGSTAPPAHA-p 1-1 5 APDTRPAPGSTAPPAHN-terminal ... peptide: the peptide Cp1 3-3 2, derived from MUC1 VNTR region (contain-ing an N-terminal cysteine to form dimers); peptides from N-...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P1
... byAnthony P. CorfieldThe MucinsGlycoproteinMethodsand Protocols Isolation of Large Gel-Forming Mucins 33From :Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The MucinsEdited ... reactivity (᭜), absorbance at 280 nm (-- -- ) , and density (). 12 Davies and Carlstedt7. The rotor type, speed and starting densities rather than the g-force are given...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P2
... T-antigen (β-d-Gal 1-3 GalNAc),but also to structures found within the backbone of oligosaccharides (β-d-Gal 1-3 /4GlcNAc) (19). Demonstration of PNA binding is not necessarily evidence of T-anti-gen ... mounting ,and its relative stability. There are, however, several other alternatives, such as 3-amino-9-ethylcarbazole (AEC), for use with horseradish peroxidase (HRP); 5-bromo-4-chloro-...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P3
... presence of competitive inhibitors of O-glycosylation, such as benzyl 2-acetamido-2-deoxy-α-D-galactopyranoside, without adversely affecting cells (9). With the eluci-dation of more sequence data for ... specificity (nonmucin glycoproteins can react, no carbohydrate or core pro-tein specificity), and lower sensitivity than slot-blot and immunoassay methods. Chap- Detection and Quantita...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P4
... 70 mL of glycerol.8. Poly-L-lysine-coated slides: slides are immersed in 0.01% (w/v) poly-L-lysine for 5 minand dried at room temperature.3. Methods3 .1. Observation and Measurementof Mucous Thickness ... L. A., Allen, A., Morris, E., and Ross-Murphy S. B. (1988) Mucus glycoproteingels: role of glycoprotein polymeric structure and carbohydrate side-chains in gel-forma-tion. Carbohydr...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P6
... with“naked” cysteine-rich domains (4–7).Histological and biochemical investigations suggested that mucous-producing tis-sues and their secretions contained a complex mixture of mucin-type glycoproteins.However, ... prepared fromthe gel-forming mucins isolated from other epithelial secretions (respiratory and cer-vical) and from intestinal cell lines (HT-29 and PC/AA). In this exampl...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P7
... Heterogeneity and Size Distribution of Gel-Forming Mucins 8787From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The MucinsEdited by: A. Corfield ... MUC5B, and MUC6 (1–4)—are gel-forming mucins, and it is possiblethat there are others. In isolating a mixture of mucins from mucus, there is consider-able scope for heterogeneity in their mass and...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P8
... 113113From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The MucinsEdited by: A. Corfield © Humana Press Inc., Totowa, NJ10Amino Acid Analysis of MucinsJun X. Yan and Nicolle ... samplesand Dr. Andrew Gooley and Prof. Keith Williams for their support.References1. Yan, J. X., Wilkins, M .R., Ou, K., Gooley, A. A., Williams, K. L., Sanchez, J.-C.,Gola...
Ngày tải lên: 08/10/2012, 10:25