... containing mM EDTA The auto-oxidation rates of Asp40Ala and Asp40Asn mutants were measured at 25 °C Oxidation of the sample was observed by Ó FEBS 2004 Haem proximal side of a haem- regulated phosphodiesterase ... binding, redox potential and auto-oxidation rates of Ec DOS PAS, and catalytic activities of full-length enzymes We found that the mutations...
Ngày tải lên: 19/02/2014, 16:20
... Jolla, CA, USA) with pET2 8a( +)-wild-type Ec DosH as a template and using the following respective 5¢-sense primers: 5¢-gatga gtcgggagACCcagctggagaaaaaag-3¢, 5¢-gatgagtcgggagTTTcag ctggagaaaaaag-3¢, ... auto-oxidation [5], whereas Ala and Asn substitutions at Asp40, an amino-acid residue that interacts via two water molecules with the proximal ligand His77, markedly increased...
Ngày tải lên: 16/03/2014, 13:20
Báo cáo khóa học: Genetic defects in fatty acid b-oxidation and acyl-CoA dehydrogenases Molecular pathogenesis and genotype–phenotype relationships ppt
... FEBS 2004 Genetic defects in fatty acid oxidation (Eur J Biochem 271) 471 Table Transporter proteins and enzymes involved in the mitochondrial saturated fatty acid oxidation FATP, fatty acid transport ... reflected in the profile of acyl-carnitines in blood and in patient cells metabolizing long-chain fatty acids [27] Not surprisingly, the severe metabolic block...
Ngày tải lên: 23/03/2014, 12:20
Báo cáo khoa học: NADH oxidation and NAD+ reduction catalysed by tightly coupled inside-out vesicles from Paracoccus denitrificans doc
... Ó FEBS 2002 NADH oxidation and NAD reduction catalysed by SPP (Eur J Biochem 269) 4021 reactions catalysed by the enzyme Unfortunately this information is ... reverse electron transfer from quinol to NAD+, driven by succinate oxidation The rates of the direct and reverse reactions depend hyperbolically on concentrations of NADH and NAD+, respectively (see ... con...
Ngày tải lên: 23/03/2014, 21:21
Báo cáo y học: " The contrasting roles of PPARδ and PPARg in regulating the metabolic switch between oxidation and storage of fats in white adipose tissue" ppt
... al.: The contrasting roles of PPARδ and PPARg in regulating the metabolic switch between oxidation and storage of fats in white adipose tissue Genome Biology 2011 12:R75 Submit your next manuscript ... contrast the roles of PPARg and PPARδ in regulating metabolism in white adipose tissue, we have Page of 19 performed a metabolo...
Ngày tải lên: 09/08/2014, 23:20
Tài liệu Báo cáo khoa học: Effect of ionic strength and oxidation on the P-loop conformation of the protein tyrosine phosphatase-like phytase, PhyAsr docx
... al Effect of ionic strength and oxidation on PhyAsr A A R258 R258 C252 OCS 252 B B R258 R258 OCS252 C252 Fig (A) Conformation of the P-loop at low ionic strength in the absence of ligand The P-loop ... Structure of PhyAsr under low and high ionic strength conditions To examine the structural effect of ionic strength on the P-loop, X...
Ngày tải lên: 18/02/2014, 18:20
Tài liệu Báo cáo Y học: Oxidation of phenols by laccase and laccase-mediator systems doc
... the results of our laccase oxidations of substrates 1–5, and the effect of the cosolvent and of the mediators on the amount of substrate metabolized by the enzyme (a) Laccase and laccase/ HPI ... the other hand, the laccase/ TEMPO system performs the selective oxidation of the hydroxymethyl groups of the substrate to aldehydes Both with laccase alone and with...
Ngày tải lên: 21/02/2014, 01:21
Báo cáo " Study on the characteristics and catalytic properties of Pt/SBA-15 in the selective oxidation of D-Glucose " potx
... was dispersed inside the pore of support material (a) 3.3 Catalytic activities of Pt/SBA-15 in the oxidation of D-glucose The results of the oxidation of D-glucose carried out on Pt/SBA-15 catalyst ... Conclusions Figure HPLC-RID image of the products of D-glucose oxidation on PS-1 catalyst On the other hand, the reaction on Pt/SBA15 cat...
Ngày tải lên: 05/03/2014, 11:21
Báo cáo khoa học: Alpha-oxidation of 3-methyl-substituted fatty acids and its thiamine dependence pptx
... and 8.0 [26] TPP -dependence of 2-HPCL Originally, 2-HPCL had been purified in the absence of TPP and the enzyme lost virtually all of its activity during purification The amino-acid sequences of ... Stereochemistry of the a-oxidation of 3-methyl-branched fatty acids in rat liver J Lipid Res 40, 601–609 Foulon, V (2001) a-Oxidation of 3-Methyl-Branched Fatty Acids: S...
Ngày tải lên: 08/03/2014, 02:20
Báo cáo khoa học: Protective effect of dietary curcumin and capsaicin on induced oxidation of low-density lipoprotein, iron-induced hepatotoxicity and carrageenan-induced inflammation in experimental rats ppt
... controls Protective effect of dietary curcumin, capsaicin and their combination on iron -induced LDL oxidation in vivo and copper -induced LDL oxidation in vitro Oxidation of LDL observed in iron(II) ... Manjunatha and K Srinivasan Protective effect of dietary curcumin, capsaicin and their combination on iron -induced LDL oxidation in viv...
Ngày tải lên: 08/03/2014, 08:20
Báo cáo khoa học: Kinetic deuterium isotope effects for 7-alkoxycoumarin O-dealkylation reactions catalyzed by human cytochromes P450 and in liver microsomes Rate-limiting C-H bond breaking in cytochrome P450 1A2 substrate oxidation pdf
... al P450 coumarin dealkylation isotope effects Table Kinetic isotope effects for 7-OR coumarin O-dealkylation by purified P450s estimated by MS Kinetic isotope effect Intermolecular competitive P450 ... system, human liver microsomes Results and discussion Intramolecular kinetic isotope effects The intramolecular kinetic isotope effects measure the...
Ngày tải lên: 16/03/2014, 13:20
Báo cáo khoa học: Protection of chylomicron remnants from oxidation by incorporation of probucol into the particles enhances their uptake by human macrophages and increases lipid accumulation in the cells ppt
... from oxidation enhances, rather than inhibits, their induction of lipid accumulation in macrophages The enhancement of lipid accumulation in macrophages by CRLPs containing probucol and lycopene ... Effect of pCRLPs on lipid synthesis in THP-1 macrophages The effects of CRLPs and pCRLPs on lipid synthesis in THP-1 macrophages were investi...
Ngày tải lên: 16/03/2014, 16:20
Báo cáo khoa học: Relationship between the structure of guanidines and N-hydroxyguanidines, their binding to inducible nitric oxide synthase (iNOS) and their iNOS-catalysed oxidation to NO pptx
... related to the binding of l-Arg [10–14] and NOHA [10], nothing is known about the thermodynamics and kinetics of the binding of guanidines and N-hydroxyguanidines to iNOS Removal of the a-amino or ... from the conversion of the LS NOS–Fe(III)–ImH complex to the HS NOS–Fe(III)–l-Arg complex was observed Inhibition of the binding of l-Arg to i...
Ngày tải lên: 16/03/2014, 22:20
Báo cáo Y học: Oxidation of propionate to pyruvate in Escherichia coli Involvement of methylcitrate dehydratase and aconitase pot
... reconstitution of the oxidation of propionyl-CoA to pyruvate by the use of purified PrpC, PrpD, AcnB and PrpB from E coli PrpD and AcnB involved in the conversion of methylcitrate into 2-methylisocitrate ... methylcitrate into 2-methylisocitrate PrpD is involved in the dehydration of (2S,3S) -methylcitrate to 2-methyl-cisaconitate The elimination of water fro...
Ngày tải lên: 17/03/2014, 10:20
Báo cáo Y học: Agmatine oxidation by copper amine oxidase Biosynthesis and biochemical characterization of N-amidino-2-hydroxypyrrolidine pdf
... Values of kcat and K are independent of the enzyme assay m Biosynthesis of N-amidino-2-hydroxypyrrolidine N-Amidino-2-hydroxypyrrolidine was synthesized as follows Twenty micrograms of P sativum copper ... of agmatine to N-amidino-2hydroxypyrrolidine was detected by 1H-NMR spectroscopy Moreover, the agmatine/ N-amidino-2-hydroxypyrrolidine stoichiometry is : as shown...
Ngày tải lên: 17/03/2014, 23:20