Dynamical simulation and structural analysis

... b-amylase (Cys83, Cys96, Cys209 and Cys344) are homologous to those found in soybean b-amylase (Cys82, Cys97, Cys208 and Cys343) On the analogy of the soybean b-amylase, the active site of the ... for the b-amylase from C sepium using the X-ray coordinates of the soybean b-amylase (Fig 4) According to the Ramachandran plot of this model the f and c...

Ngày tải lên: 22/02/2014, 07:20

... we have purified, cloned and characterized Cyn d 24 as a novel pathogenesis-related protein from BGP Additionally, the identification of Cyn d 24 has identified the involvement of a novel class of ... AGAD AADA.NA.VG D. D 113 Zea AYA.S A- QRQG LI GG FW AGAD.SASDA.GS.VS QY.DHDT.S 112 Nicotiana AYA.N S-Q.AA NL HGQ AE -GDFMTAAKA.EM.V QY.DHD 118 Cyn d 24 DQGKM...

Ngày tải lên: 07/03/2014, 12:20

... of NHase seems to form a disulfide bond easily under oxidative conditions, if the site contains no metal ion The metal activator of NHase may incorporate a metal ion to prevent the formation of ... & Endo, I (1999) Functional expression of nitrile hydratase in Escherichia coli: requirement of a nitrile hydratase activator and post-translational modification of a liga...

Ngày tải lên: 16/03/2014, 16:20

... dehydrogenase and prephenate dehydratase catalyze the biosynthesis of tyrosine and phenylalanine, respectively As this biosynthetic pathway is absent in mammals but is essential for the survival of bacteria ... Reddy PT (2006) Biochemical and structural characterization of the secreted chorismate mutase (Rv1885c) from Mycobacterium tuberculosis H37Rv: an *Ar...

Ngày tải lên: 17/03/2014, 17:20

... SU B Validation of Communications Systems with SDL: The Art of SDL Simulation and Reachability Analysis Laurent Doldi  2003 John Wiley & Sons, Ltd ISBN: 0-470-85286-0 Simpo26 Validation of Communications ... of the specification before the target hardware and software platform is available: board, board support package, compiler and so on Validati...

Ngày tải lên: 27/06/2014, 00:20

... and attachment of pea seeds to the replum in a pod of the def mutant pea The def mutant pea shows a swollen and thick funicle compared to the wild type Arrows indicate the AZ and ALZ in the wild ... growing of the plants, harvested materials, carried out the structural examination and drafted the manuscript YKL participated in designing t...

Ngày tải lên: 12/08/2014, 03:20

... residues, the other of tyrosines This grouping is based on the similar characteristics of serine and threonine, and the fact that they are usually targeted by the same protein kinases The study focused ... identity cut-off (>30%) very few sites were highly conserved (Figure 7a) Only less than 5% of the sites were strictly conserved across the alignments, and...

Ngày tải lên: 14/08/2014, 07:21

... vertebrate BTB- ZF and BBK proteins are more closely related, and cannot be separated by BLASTCLUST Long form of the BTB domain The majority of BTB domains from the BTB- ZF, BBK, MATHBTB, RhoBTB and BTB- basic ... some BTB- ankyrin proteins are composed of an amino-terminal BTB domain, a central helical region, 19 ankyrin repeats and a carboxy-terminal FYVE domain...

Ngày tải lên: 14/08/2014, 14:22

... R G IQLR L FYNVT YT AYRLS P L FGF EYSNL TEIG KKL TRF L RY GTDRT GYFTN STRF LLHKQ WT LYSLT P L YKF SYSN- - L KEY SR L L NA FIVAE KQ K G LAVE V LLHKQ WT IYSLT P L YKF SYSN- - L KDY SR L L SA ... SSRTEFFISALLNYGLYKD FTLRDLLRPDP RRLVQVLSALINFLYYRD YTYMDIIKPA VKKTLATLSYLFNHLAYYK 40% 3% 10% 26% scNdc80 Fungi Metazoa Plantae Newly annotated protein QELLEYLTHNNFELEMKHSLGQNTLRSP TQKDFNYIFQWLYHRIDPGYRF...

Ngày tải lên: 14/08/2014, 16:21

... demonstrate the plasticity of substrate recognition by caspase-7, and will be valuable for the design of inhibitors of this pharmacologically important enzyme Results Inhibition of caspase-7 by tetrapeptide ... et al Plasticity of caspase-7 specificity pockets was blocked in the crystal structure of caspase-3 in complex with the inhibitor of apoptosis protein X...

Ngày tải lên: 18/02/2014, 16:20

... to the cytosol Here we confirm and extend earlier studies of these AAA-peroxins and give a further detailed functional analysis of their cassette structure and interaction The interaction of Pex1p ... for the Pex1p Pex6p interaction and their functional role in peroxisome biogenesis Results The contribution of Pex1p and Pex6p to peroxisomal bioge...

Ngày tải lên: 07/03/2014, 16:20

... mode of the mixture of oligosaccharides prior to HPAEC, of the isolated main oligosaccharide of deacylated LPS and of acylated purified LPS from E coli F2513 (R4 core-type) In addition, the deacylated ... LPS (R4 core-type) is as depicted in Fig Structural analysis of E coli F653 core-oligosaccharides (R3 core) We have subjected purified LPS from E c...

Ngày tải lên: 08/03/2014, 09:20

... (Stratagene, La Jolla, CA, USA) The primers used were: 5¢-TATATCATTCA GGATTATTTGTATCTTTTAGAATACGCTAAGGTG-3 ¢ (forward, the mutagenesis codon underlined) and 5¢-TT AGCGTATTCTAAAAGATACAAATAATCCTGAATGA ... Structure of H pylori TenA N Barison et al A B Fig TenA active site (A) Cartoon view of a detail of TenA active site The side chains of residues relevant for cataly...

Ngày tải lên: 16/03/2014, 00:20

... Structure of the human Cripto CFC domain (Eur J Biochem 270) 3615 Fig Alignment of the sequences of the CFC domain of human Cripto and PMP-C Conserved residues are framed with solid lines and homologous ... types of mutants, i.e the CFC domain mutant, H120G/W123G, and the EGF domain mutant, N85G/T88A, to bind to ALK4 Comparative modeling The 3-D s...

Ngày tải lên: 23/03/2014, 21:20

... b5 strand) of D3 and F27/Y289 (b2 strand), L67/M324, V70/I327 and L72/L328 (all in b4 strand) of B The second cluster consists of P6/M267, L10/ L271 (both in a-helix), V18/C279 (b1 strand), L32/F293 ... solution structures of the UBL domain of hHR23A/B bound to a UIM peptide of S5a [99,119] could be used to model the complex of hHR23A/B and ataxin-3 Similarly, the comple...

Ngày tải lên: 30/03/2014, 15:20